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http://dx.doi.org/10.4062/biomolther.2014.075

Genistein from Vigna angularis Extends Lifespan in Caenorhabditis elegans  

Lee, Eun Byeol (College of Pharmacy, Woosuk University)
Ahn, Dalrae (College of Pharmacy, Woosuk University)
Kim, Ban Ji (College of Pharmacy, Woosuk University)
Lee, So Yeon (College of Pharmacy, Woosuk University)
Seo, Hyun Won (College of Pharmacy, Woosuk University)
Cha, Youn-Soo (Department of Food Science and Human Nutrition, Chonbuk National University)
Jeon, Hoon (College of Pharmacy, Woosuk University)
Eun, Jae Soon (College of Pharmacy, Woosuk University)
Cha, Dong Seok (College of Pharmacy, Woosuk University)
Kim, Dae Keun (College of Pharmacy, Woosuk University)
Publication Information
Biomolecules & Therapeutics / v.23, no.1, 2015 , pp. 77-83 More about this Journal
Abstract
The seed of Vigna angularis has long been cultivated as a food or a folk medicine in East Asia. Genistein (4',5,7-trihydroxyisoflavone), a dietary phytoestrogen present in this plant, has been known to possess various biological properties. In this study, we investigated the possible lifespan-extending effects of genistein using Caenorhabditis elegans model system. We found that the lifespan of nematode was significantly prolonged in the presence of genistein under normal culture condition. In addition, genistein elevated the survival rate of nematode against stressful environment including heat and oxidative conditions. Further studies demonstrated that genistein-mediated increased stress tolerance of nematode could be attributed to enhanced expressions of stress resistance proteins such as superoxide dismutase (SOD-3) and heat shock protein (HSP-16.2). Moreover, we failed to find genistein-induced significant change in aging-related factors including reproduction, food intake, and growth, indicating genistein exerts longevity activity independent of affecting these factors. Genistein treatment also led to an up-regulation of locomotory ability of aged nematode, suggesting genistein affects healthspan as well as lifespan of nematode. Our results represent that genistein has beneficial effects on the lifespan of C. elegans under both of normal and stress condition via elevating expressions of stress resistance proteins.
Keywords
Vigna angularis; Genistein; Caenorhabditis elegans; Lifespan extension; Stress tolerance;
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