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http://dx.doi.org/10.4062/biomolther.2014.086

Effect of Cordycepin-Enriched WIB801C from Cordyceps militaris Suppressing Fibrinogen Binding to Glycoprotein IIb/IIIa  

Lee, Dong-Ha (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University)
Kim, Hyun-Hong (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University)
Lim, Deok Hwi (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University)
Kim, Jong-Lae (Natural Product Research Team, Central Research Center, Whanin Pharm. Co., Ltd.)
Park, Hwa-Jin (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University)
Publication Information
Biomolecules & Therapeutics / v.23, no.1, 2015 , pp. 60-70 More about this Journal
Abstract
In this study, we investigated the effects of cordycepin-enriched (CE)-WIB801C, a n-butanol extract of Cordyceps militaris-hypha on collagen-stimulated platelet aggregation. CE-WIB801C dose dependently inhibited collagen-induced platelet aggregation, and had a synergistic effect together with cordycepin (W-cordycepin) from CE-WIB801C on the inhibition of collagen-induced platelet aggregation. CE-WIB801C and cordycepin stimulated the phosphorylation of VASP ($Ser^{157}$) and the dephosphorylation of PI3K and Akt, and inhibited the binding of fibrinogen to glycoprotein IIb/IIIa (${\alpha}IIb/{\beta}3$) and the release of ATP and serotonin in collagen-induced platelet aggregation. A-kinase inhibitor Rp-8-Br-cAMPS reduced CE-WIB801C-, and cordycepin-increased VASP ($Ser^{157}$) phosphorylation, and increased CE-WIB801C-, and cordycepin-inhibited the fibrinogen binding to ${\alpha}IIb/{\beta}3$. Therefore, we demonstrate that CE-WIB801C-, and cordycepin-inhibited fibrinogen binding to ${\alpha}IIb/{\beta}3$are due to stimulation of cAMP-dependent phosphorylation of VASP ($Ser^{157}$), and inhibition of PI3K/Akt phosphorylation. These results strongly indicate that CE-WIB801C and cordycepin may have preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.
Keywords
CE-WIB801C; Cordycepin; VASP ($Ser^{157}$); PI3K/Akt; Fibrinogen binding;
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