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http://dx.doi.org/10.4062/biomolther.2014.052

Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-κB and MAPK Activation  

Cho, Byoung Ok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
So, Yangkang (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Lee, Chang Wook (Department of Food Science and Human Nutrition, Chungnam National University)
Jin, Chang Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Yook, Hong Sun (Department of Food Science and Human Nutrition, Chungnam National University)
Jeong, Yong Wook (Department of Microbiology, College of Medicine, Seonam University)
Park, Jong Chun (Department of Microbiology, College of Medicine, Seonam University)
Jeong, Il Yun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Publication Information
Biomolecules & Therapeutics / v.22, no.4, 2014 , pp. 288-294 More about this Journal
Abstract
Mangostenone F (MF) is a natural xanthone isolated from Garcinia mangostana. However, little is known about the biological activities of MF. This study was designed to investigate the anti-inflammatory effect and underlying molecular mechanisms of MF in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. MF dose-dependently inhibited the production of NO, iNOS, and pro-inflammatory cytokines (TNF-${\alpha}$, IL-6, and IL-$1{\beta}$) in LPS-stimulated RAW264.7 macrophages. Moreover, MF decreased the NF-${\kappa}B$ luciferase activity and NF-${\kappa}B$ DNA binding capacity in LPS-stimulated RAW264.7 macrophages. Furthermore, MF suppressed the NF-${\kappa}B$ activation by inhibiting the degradation of $I{\kappa}B{\alpha}$ and nuclear translocation of p65 subunit of NF-${\kappa}B$. In addition, MF attenuated the AP-1 luciferase activity and phosphorylation of ERK, JNK, and p38 MAP kinases. Taken together, these results suggest that the anti-inflammatory effect of MF is associated with the suppression of NO production and iNOS expression through the down-regulation of NF-${\kappa}B$ activation and MAPK signaling pathway in LPS-stimulated RAW264.7 macrophages.
Keywords
Mangostenone F; NO; iNOS; NF-${\kappa}B$; MAPK;
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