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http://dx.doi.org/10.4062/biomolther.2013.096

Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells  

Cho, Kyoungwon (College of Pharmacy, Chungnam National University)
Song, Seok Bean (College of Pharmacy, Chungnam National University)
Nguyen, Huu Tung (College of Pharmacy, Chungnam National University)
Kim, Kyoon Eon (Department of Biochemistry, Chungnam National University)
Kim, Young Ho (College of Pharmacy, Chungnam National University)
Publication Information
Biomolecules & Therapeutics / v.22, no.1, 2014 , pp. 55-61 More about this Journal
Abstract
Panax ginseng is a medicinal herb that is used worldwide. Its medicinal effects are primarily attributable to ginsenosides located in the root, leaf, seed, and flower. The flower buds of Panax ginseng (FBPG) are rich in various bioactive ginsenosides, which exert immunomodulatory and anti-inflammatory activities. The aim of the present study was to assess the effect of 18 ginsenosides isolated from steamed FBPG on the transcriptional activity of NF-${\kappa}B$ and the expression of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-stimulated target genes in liver-derived cell lines. Noticeably, the ginsenosides $Rk_3$ and $Rs_4$ exerted the strongest activity, inhibiting NF-${\kappa}B$ in a dose-dependent manner. SF and $Rg_6$ also showed moderately inhibitory effects. Furthermore, these four compounds inhibited the TNF-${\alpha}$-induced expression of IL8, CXCL1, iNOS, and ICAM1 genes. Consequently, ginsenosides purified from steamed FBPG have therapeutic potential in TNF-${\alpha}$-mediated diseases such as chronic hepatic inflammation.
Keywords
NF-${\kappa}B$ inhibitory activity; Panax ginseng flower buds; Tumor necrosis factor-${\alpha}$; Hepatocyte derived cells;
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