Browse > Article
http://dx.doi.org/10.4062/biomolther.2013.067

Neuroprotective Effects of a Butanol Fraction of Rosa hybrida Petals in a Middle Cerebral Artery Occlusion Model  

Yang, Goeun (College of Veterinary Medicine, Chungbuk National University)
Park, Dongsun (College of Veterinary Medicine, Chungbuk National University)
Lee, Sun Hee (College of Veterinary Medicine, Chungbuk National University)
Bae, Dae-Kwon (College of Veterinary Medicine, Chungbuk National University)
Yang, Yun-Hui (College of Veterinary Medicine, Chungbuk National University)
Kyung, Jangbeen (College of Veterinary Medicine, Chungbuk National University)
Kim, Dajeong (College of Veterinary Medicine, Chungbuk National University)
Choi, Ehn-Kyoung (College of Veterinary Medicine, Chungbuk National University)
Hong, Jin Tae (College of Pharmacy, Chungbuk National University)
Jeong, Heon-Sang (Department Food Science and Technology, Chungbuk National University)
Kim, Hee Jung (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University)
Jang, Su Kil (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University)
Joo, Seong Soo (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University)
Kim, Yun-Bae (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Biomolecules & Therapeutics / v.21, no.6, 2013 , pp. 454-461 More about this Journal
Abstract
The neuroprotective effects of a butanol fraction of white rose petal extract (WRPE-BF) were investigated in a middle cerebral artery occlusion (MCAO) model. Seven week-old male rats were orally administered WRPE-BF for 2 weeks and subjected to MCAO for 2 h, followed by reperfusion. Twenty-four h later, MCAO-induced behavioral dysfunctions were markedly improved in a dose-dependent manner by pretreatment with WRPE-BF. Moreover, higher dose of WRPE-BF not only decreased infarction area but also effectively reduced astrogliosis. The expression of inducible nitric oxide synthase, cyclooxygenase-2, and glial fibrillary acidic protein in MCAO model were markedly inhibited by WRPE-BF treatment. Notably, WRPE-BF decreased nitricoxide and malondialdehyde levels in the striatum and subventricular zone of stroke-challenged brains. These data suggested that WRPE-BF may exert its neuroprotective effects via anti-oxidative and anti-inflammatory activities against ischemia-reperfusion brain injury and could be a good candidate as a therapeutic target for ischemic stroke.
Keywords
Ischemic brain injury; Middle cerebral artery occlusion; Glial fibrillary acidic protein; Rosa hybrida; Antioxidation; Anti-inflammation;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Asano, T., Mori, T., Shimoda, T., Shinagawa., R., Satoh, S., Yada, N., Katsumata, S., Matsuda, S., Kagamiishi, Y. and Tateishi, N. (2005) Arundic acid (ONO-2506) ameliorates delayed ischemic brain damage by preventing astrocytic overproduction of S100B. Curr. Drug Targets CNS Neurol. Disord. 4, 127-142.   DOI
2 Callaway, J. K., Beart, P. M. and Jarrott, B. (1998) A reliable procedure for comparison of antioxidants in rat brain homogenates. J. Pharmacol. Toxicol. Methods 39, 155-162.   DOI   ScienceOn
3 Chan, P. H. (1996) Role of oxidants in ischemic brain damage. Stroke 27, 1124-1129.   DOI   ScienceOn
4 Chan, P. H. (2001) Reactive oxygen radicals in signaling and damage in the ischemic brain. J. Cereb. Blood Flow Metab. 21, 2-14.
5 Cho, E. J., Yokozawa, T., Rhyu, D. Y., Kim, S. L., Shibahara, N. and Park, J. C. (2003) Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical. Phytomedicine 10, 544-551.   DOI   ScienceOn
6 Choi, B. I., Park, D., Lee, S. H., Bae, D. K., Yang, G., Yang, Y. H., Kim, T. K., Choi, E. K., Lee, H. J., Choi, K. C., Nahm, S. S. and Kim, Y. B. (2012) Neurobehavioral defi cits correlate with the cerebral infarction volume of stroke animals: a comparative study on ischemia-reperfusion and photothrombosis models. Environ. Toxicol. Pharmacol. 33, 60-69.   DOI   ScienceOn
7 Clark, W. M., Rinker, L. G., Lessov, N. S., Lowery, S. L. and Cipolla, M. J. (2001) Efficacy of antioxidant therapies in transient focal ischemia in mice. Stroke 32, 1000-1004.   DOI   ScienceOn
8 Collaço-Moraes, Y., Aspey, B., Harrison, M. and de Belleroche, J. (1996) Cyclo-oxygenase-2 messenger RNA induction in focal cerebral ischemia. J. Cereb. Blood Flow Metab. 16, 1366-1372.   DOI
9 Iadecola, C. and Alexander, M. (2001) Cerebral ischemia and infl ammation. Curr. Opin. Neurol. 14, 89-94.   DOI   ScienceOn
10 Iadecola, C., Zhang, F., Casey, R., Nagayama, M. and Ross, M. E. (1997) Delayed reduction of ischemic brain injury and neurological deficits in mice lacking the inducible nitric oxide synthase gene. J. Neurosci. 17, 9157-9164.
11 Jeon, J. H., Kwon, S-C., Park, D. S., Shin, S., Jeong, J. H., Park, S. Y., Hwang, S. Y., Kim, Y. B. and Joo, S. S. (2009) Anti-allergic effects of white rose pertal extract and anti-atopic properties of its hexane fraction. Arch. Pharm. Res. 32, 823-830.   DOI   ScienceOn
12 Joo, S. S., Kim, Y. B. and Lee, D. I. (2010) Antimicrobial and antioxidant properties of secondary metabolites from white rose flower. Plant Pathol. J. 26, 57-62.   DOI   ScienceOn
13 Kondo, K., Kurihara, M., Miyata, N., Suzuki, T. and Toyoda, M. (1999) Scavenging mechanisms of (-)-epigallocatechin gallate and (-)-epicatechin gallate on peroxyl radicals and formation of superoxide during the inhibitory action. Free Radic. Biol. Med. 27, 855-863.   DOI   ScienceOn
14 Lakhan, S. E., Kirchgessner, A. and Hofer, M. (2009) Inflammatory mechanisms in ischemic stroke: therapeutic approaches. J. Transl. Med. 7, 97.   DOI   ScienceOn
15 Lee, H. J., Kim, H. S., Kim, S. T., Park, D., Hong, J. T., Kim, Y. B. and Joo, S. S. (2011) Anti-inflammatory effects of hexane fraction from white rose flower extracts via inhibition of inflammatory repertoires. Biomol. Ther. 19, 331-335.   DOI   ScienceOn
16 Mojsilovic-Petrovic, J., Callaghan, D., Cui, H., Dean, C., Stanimirovic, D. B. and Zhang, W. (2007) Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes. J. Neuroinflammation 4, 12.   DOI   ScienceOn
17 Longa, E. Z., Weinstein, P. R., Carlson, S. and Cummins, R. (1989) Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20, 84-91.   DOI   ScienceOn
18 Margaill, I., Plotkine, M. and Lerouet, D. (2005) Antioxidant strategies in the treatment of stroke. Free Radic. Biol. Med. 39, 429-443.   DOI   ScienceOn
19 Mohammadi, M. T., Shid-Moosavi, S. M. and Dehghani, G. A. (2012) Contribution of nitric oxide synthase (NOS) in blood-brain barrier disruption during acute focal cerebral ischemia in normal rat. Pathophysiology 19, 13-20.   DOI   ScienceOn
20 Ng, T. B., Gao, W., Li, L., Niu, S. M., Zhao, L., Liu, J., Shi, L. S., Fu, M. and Liu, F. (2005) Rose (Rosa rugosa)-flower extract increases the activities of antioxidant enzymes and their gene expression and reduces lipid peroxidation. Biochem. Cell Biol. 83, 78-85.   DOI
21 Nicolis, E., Lampronti, I., Dechecchi, M. C., Borgatti, M., Tamanini, A., Bianchi, N., Bezzerri, V., Mancini, I., Giri, M. G., Rizzotti, P., Gambari, R. and Cabrini, G. (2008) Pyrogallol, an active compound from the medicinal plant Emblica offi cinalis, regulates expression of pro-inflammatory genes in bronchial epithelial cells. Int. Immunopharmacol. 8, 1672-1680.   DOI   ScienceOn
22 Nogawa, S., Forster, C., Zhang, F., Nagayama, M., Ross, M. E. and Iadecola, C. (1998) Interaction between inducible nitric oxide synthase and cyclooxygenase-2 after cerebral ischemia. Proc. Natl. Acad. Sci. U.S.A. 95, 10966-10971.   DOI   ScienceOn
23 Nogawa, S., Zhang, F., Ross, M. E. and Iadecola, C. (1997) Cyclooxygenase-2 gene expression in neurons contributes to ischemic brain damage. J. Neurosci. 17, 2746-2755.
24 Park, D., Joo, S. S., Lee, H., Choi, K. C., Kim, S. U. and Kim, Y. B. (2012) Microtubule-associated protein 2, an early blood marker of ischemic brain injury. J. Neurosci. Res. 90, 461-467.   DOI   ScienceOn
25 Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358.   DOI   ScienceOn
26 Pan, H. C., Kao, T. K., Ou, Y. C., Yang, D. Y., Yen, Y. J., Wang, C. C., Chuang, Y. H., Liao, S. L., Raung, S. L., Wu, C. W., Chiang, A. N. and Chen, C. J. J. (2009) Protective effect of docosahexaenoic acid against brain injury in ischemic rats. J. Nutr. Biochem. 20, 715-725.   DOI   ScienceOn
27 Park, D., Jeon, J. H., Kwon, S. C., Shin, S., Jang, J. Y., Jeong, H. S., Lee, D. I., Kim, Y. B. and Joo, S. S. (2009) Antioxidative activities of white rose flower extract and pharmaceutical advantages of its hexane fraction via free radical scavenging effects. Biochem. Cell Biol. 87, 943-952.   DOI   ScienceOn
28 Park, D., Lee, S. H., Choi, Y. J., Bae, D. K., Yang, Y. H., Yang, G., Kim, T. K., Yeon, S., Hwang, S. Y., Joo, S. S. and Kim, Y.B. (2011) Improving effect of silk peptides on the cognitive function of rats with aging brain facilitated by D-galactose. Biomol. Ther. 19, 224-230.   과학기술학회마을   DOI   ScienceOn
29 Raghubir, R. (2008) Emerging role of astrocytes in cerebral ischemia/ reperfusion injury. Ann. Neurosci. 15, 17-24.   DOI
30 Rogers, D. C. and Hunter, A. J. (1997) Photothrombotic lesions of the rat cortex impair acquisition of the water maze. Pharmacol. Biochem. Behav. 56, 747-754.   DOI   ScienceOn
31 Vaughan, C. J. and Delanty, N. (1999) Neuroprotective properties of statins in cerebral ischemia and stroke. Stroke 30, 1969-1973.   DOI   ScienceOn
32 Schmid-Elsaesser, R., Zausinger, S., Hungerhuber, E., Baethmann, A. and Reulen, H. J. (1998) A critical reevaluation of the intraluminal thread model of focal cerebral ischemia: evidence of inadvertent premature reperfusion and subarachnoid hemorrhage in rats by laser-Doppler flowmetry. Stroke 29, 2162-2170.   DOI   ScienceOn
33 Sims, N. R. and Muyderman, H. (2010) Mitochondria, oxidative metabolism and cell death in stroke. Biochim. Biophys. Acta 1802, 80-91.   DOI   ScienceOn
34 Thaakur, S. and Sravanthi, R. (2010) Neuroprotective effect of Spirulina in cerebral ischemia-reperfusion injury in rats. J. Neural Transm. 117, 1083-1091.   DOI   ScienceOn