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http://dx.doi.org/10.4062/biomolther.2013.051

Inhibitory Effect of an Urotensin II Receptor Antagonist on Proinflammatory Activation Induced by Urotensin II in Human Vascular Endothelial Cells  

Park, Sung Lyea (Department of Pathophysiology, College of Pharmacy, Ajou University)
Lee, Bo Kyung (Department of Pathophysiology, College of Pharmacy, Ajou University)
Kim, Young-Ae (Department of Pathophysiology, College of Pharmacy, Ajou University)
Lee, Byung Ho (Bio-Organic Science Division, Korea Research Institute of Chemical Technology)
Jung, Yi-Sook (Department of Pathophysiology, College of Pharmacy, Ajou University)
Publication Information
Biomolecules & Therapeutics / v.21, no.4, 2013 , pp. 277-283 More about this Journal
Abstract
In this study, we investigated the effects of a selective urotensin II (UII) receptor antagonist, SB-657510, on the inflmmatory response induced by UII in human umbilical vein endothelial cells (EA.hy926) and human monocytes (U937). UII induced inflammatory activation of endothelial cells through expression of proinflammatory cytokines (IL-$1{\beta}$ and IL-6), adhesion molecules (VCAM-1), and tissue factor (TF), which facilitates the adhesion of monocytes to EA.hy926 cells. Treatment with SB-657510 significantly inhibited UII-induced expression of IL-$1{\beta}$, IL-6, and VCAM-1 in EA.hy926 cells. Further, SB-657510 dramatically blocked the UII-induced increase in adhesion between U937 and EA.hy926 cells. In addition, SB-657510 remarkably reduced UII-induced expression of TF in EA.hy926 cells. Taken together, our results demonstrate that the UII antagonist SB-657510 decreases the progression of inflammation induced by UII in endothelial cells.
Keywords
Urotensin II; Urotensin II receptor antagonist; Adhesion molecules; Cytokines; Tissue factor; Endothelial cells;
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