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http://dx.doi.org/10.4062/biomolther.2013.002

Administration of Phytoceramide Enhances Memory and Up-regulates the Expression of pCREB and BDNF in Hippocampus of Mice  

Lee, Yeonju (Department of Neuroscience and Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University)
Kim, Jieun (Department of Neuroscience and Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University)
Jang, Soyong (Department of Neuroscience and Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University)
Oh, Seikwan (Department of Neuroscience and Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University)
Publication Information
Biomolecules & Therapeutics / v.21, no.3, 2013 , pp. 229-233 More about this Journal
Abstract
This study was aimed at investigating the possible effects of phytoceramide (Pcer) on learning and memory and their underlying mechanisms. Phytoceramide was orally administered to ICR mice for 7 days. Memory performances were assessed using the passive avoidance test and Y-maze task. The expressions of phosphorylated cAMP response element binding protein (pCREB), brain-derived neurotrophic factor (BDNF) were measured with immunoblot. The incorporation of 5-bromo-2-deoxyuridine (BrdU) in hippocampal regions was investigated by using immunohistochemical methods. Treatment of Pcer enhanced cognitive performances in the passive avoidance test and Y-maze task. Immunoblotting studies revealed that the phosphorylated CREB and BDNF were significantly increased on hippocampus in the Pcer-treated mice. Immunohistochemical studies showed that the number of immunopositive cells to BrdU was significantly increased in the hippocampal dentate gyrus regions after Pcer-treatment for 7 days. These results suggest that Pcer contribute to enhancing memory and BDNF expression and it could be secondary to the elevation of neurogenesis.
Keywords
Memory; Neurogenesis; Phytoceramide; Y-maze;
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