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http://dx.doi.org/10.4062/biomolther.2013.023

Aromadendrin Inhibits Lipopolysaccharide-Induced Nuclear Translocation of NF-κB and Phosphorylation of JNK in RAW 264.7 Macrophage Cells  

Lee, Jae-Won (Department of Pharmacology, College of Medicine, Kangwon National University)
Kim, Nam Ho (Department of Pharmacology, College of Medicine, Kangwon National University)
Kim, Ji-Young (Department of Pharmacology, College of Medicine, Kangwon National University)
Park, Jun-Ho (Department of Pharmacology, College of Medicine, Kangwon National University)
Shin, Seung-Yeon (Department of Pharmacology, College of Medicine, Kangwon National University)
Kwon, Yong-Soo (College of Pharmacy, Kangwon National University)
Lee, Hee Jae (Department of Pharmacology, College of Medicine, Kangwon National University)
Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University)
Chun, Wanjoo (Department of Pharmacology, College of Medicine, Kangwon National University)
Publication Information
Biomolecules & Therapeutics / v.21, no.3, 2013 , pp. 216-221 More about this Journal
Abstract
Aromadendrin, a flavonol, has been reported to possess a variety of pharmacological activities such as anti-inflammatory, antioxidant, and anti-diabetic properties. However, the underlying mechanism by which aromadendrin exerts its biological activity has not been extensively demonstrated. The objective of this study is to elucidate the anti-inflammatory mechanism of aromadedrin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Aromadendrin significantly suppressed LPS-induced excessive production of pro-inflammatory mediators such as nitric oxide (NO) and $PGE_2$. In accordance, aromadendrin attenuated LPS-induced overexpression iNOS and COX-2. In addition, aromadendrin significantly suppressed LPS-induced degradation of $I{\kappa}B$, which sequesters NF-${\kappa}B$ in cytoplasm, consequently inhibiting the nuclear translocation of pro-inflammatory transcription factor NF-${\kappa}B$. To elucidate the underlying signaling mechanism of anti-inflammatory activity of aromadendrin, MAPK signaling pathway was examined. Aromadendrin significantly attenuated LPS-induced activation of JNK, but not ERK and p38, in a concentration-dependent manner. Taken together, the present study clearly demonstrates that aromadendrin exhibits anti-inflammatory activity through the suppression of nuclear translocation of NF-${\kappa}B$ and phosphorylation of JNK in LPS-stimulated RAW 264.7 macrophage cells.
Keywords
Aromadendrin; COX-2; iNOS; JNK; Lipopolysaccharide; NF-${\kappa}B$; RAW 264.7 cells;
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