[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4062/biomolther.2012.091

3,4,5-Trihydroxycinnamic Acid Inhibits Lipopolysaccharide-Induced Inflammatory Response through the Activation of Nrf2 Pathway in BV2 Microglial Cells

Lee, Jae-Won (Department of Pharmacology, College of Medicine, Kangwon National University)
Choi, Yong-Jun (Department of Pharmacology, College of Medicine, Kangwon National University)
Park, Jun-Ho (Department of Pharmacology, College of Medicine, Kangwon National University)
Sim, Jae-Young (College of Pharmacy, Kangwon National University)
Kwon, Yong-Soo (College of Pharmacy, Kangwon National University)
Lee, Hee Jae (Department of Pharmacology, College of Medicine, Kangwon National University)
Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University)
Chun, Wanjoo (Department of Pharmacology, College of Medicine, Kangwon National University)

Publication Information

Biomolecules & Therapeutics / v.21, no.1, 2013 , pp. 60-65 More about this Journal

Abstract

3,4,5-Trihydroxycinnamic acid (THC) is a derivative of hydroxycinnamic acids, which have been reported to possess a variety of biological properties such as anti-inflammatory, anti-tumor, and neuroprotective activities. However, biological activity of THC has not been extensively examined. Recently, we reported that THC possesses anti-inflammatory activity in LPS-stimulated BV2 microglial cells. However, its precise mechanism by which THC exerts anti-inflammatory action has not been clearly identified. Therefore, the present study was carried out to understand the anti-inflammatory mechanism of THC in BV2 microglial cells. THC effectively suppressed the LPS-induced induction of pro-inflammatory mediators such as NO, TNF- ${\alpha}$ , and IL- $1{\beta}$ . THC also suppressed expression of MCP-1, which plays a key role in the migration of activated microglia. To understand the underlying mechanism by which THC exerts these anti-inflammatory properties, involvement of Nrf2, which is a cytoprotective transcription factor, was examined. THC resulted in increased phosphorylation of Nrf2 with consequent expression of HO-1 in a concentration-dependent manner. THC-induced phosphorylation of Nrf2 was blocked with SB203580, a p38 MAPK inhibitor, indicating that p38 MAPK is the responsible kinase for the phosphorylation of Nrf2. Taken together, the present study for the first time demonstrates that THC exerts anti-inflammatory properties through the activation of Nrf2 in BV2 microglial cells, suggesting that THC might be a valuable therapeutic adjuvant for the treatment of inflammation-related disorders in the CNS.

Keywords

Neuro-inflammation; 3,4,5-trihydroxycinnamic acid; Nrf2; Heme oxygenase-1;

Citations & Related Records

Times Cited By KSCI : 3 (Citation Analysis)

Reference
Cited By KSCI

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1	Methyl p-Hydroxycinnamate Suppresses Lipopolysaccharide-Induced Inflammatory Responses through Akt Phosphorylation in RAW264.7 Cells / [Vo, Van Anh;Lee, Jae-Won;Shin, Seung-Yeon;Kwon, Jae-Hyun;Lee, Hee Jae;Kim, Sung-Soo;Kwon, Yong-Soo;Chun, Wanjoo;] / Biomolecules & Therapeutics
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