Browse > Article
http://dx.doi.org/10.4062/biomolther.2012.20.1.075

Effects of Ginsenosides Rg1 on Osteoblasts Cultured with Ti Particles  

Lin, Yu (Chinese Orthopedics and Traumatology College, Fujian University of Traditional Chinese Medicine)
Wu, Yinsheng (Bone Disease Research Institute, Integrative Medicine Institute, Fujian University of Traditional Chinese Medicine)
He, Jiacheng (Chinese Orthopedics and Traumatology College, Fujian University of Traditional Chinese Medicine)
Huang, Yunmei (Bone Disease Research Institute, Integrative Medicine Institute, Fujian University of Traditional Chinese Medicine)
Lin, Yanping (Bone Disease Research Institute, Integrative Medicine Institute, Fujian University of Traditional Chinese Medicine)
Publication Information
Biomolecules & Therapeutics / v.20, no.1, 2012 , pp. 75-80 More about this Journal
Abstract
The aim of this study was to explore the role and effect of ginsenosides Rg1 on osteoblasts cultured with Ti particles. Osteoblasts from neonatal rats were cultured with particles and different doses of Rg1, the main active ingredient in ginsenosides Rg1. We found that the COX-2, $PGE_2$, TNF-${\alpha}$, IL-1, and IL -6 concentrations in the medium of cells cultured with Ti particles significantly increased as compared with that of the control cells (p<0.05 or p<0.01). In addition, cells cultured with Ti particles alone exhibited the highest concentrations of these molecules. The $PGE_2$, TNF-${\alpha}$, IL-1, and IL-6 levels in the medium of cells cultured with Rg1 were in between those of the control cells and the cells cultured with Ti particles alone. The IL-1ra level in the group cultured with Ti and medium-dose Rg1 was the highest followed by the cells cultured with Ti and high-dose Rg1 and those cultured with Ti and low-dose Rg1 (p<0.05). In conclusion, ginsenosides can reduce the levels of infl ammatory cytokines produced by osteoblasts on induction with Ti particles and can prevent prosthesis loosening.
Keywords
Osteoblasts; Ginsenosides Rg1; Ti particles; Prosthesis loosening; Inflammatory factors;
Citations & Related Records
 (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Abrahamsen, B., Shalhoub, V., Larson, E. K., Eriksen, E. F., Beck- Nielsen, H. and Marks, S. C. Jr. (2000) Cytokine RNA levels in transiliac bone biopsies from healthy early postmenopausal women. Bone. 26, 137-145.   DOI   ScienceOn
2 Burger, D., Chicheportiche, R., Giri, J. G. and Dayer, J. M. (1995) The inhibitory activity of human interleukin-1 receptor antagonist is enhanced by type II interleukin-1 soluble receptor and hindered by type I interleukin-1 soluble receptor. J. Clin. Invest. 96, 38-41.   DOI   ScienceOn
3 Chen, L. L. and Yan, J. (2001) Porphyromonas gingivalis lipopolysaccharide activated bone resorption of osteoclasts by inducing IL-1, TNF, and PGE. Acta. Pharmacol. Sin. 22, 614-618.
4 Chen, X. (1996) Cardiovascular protection by ginsenosides and their nitric oxide releasing action. Clin. Exp. Pharmacol. Physiol. 23, 728-732.   DOI   ScienceOn
5 Crotti, T. N., Smith, M. D., Findlay, D. M., Zreiqat, H., Ahern, M. J., Weedon, H., Hatzinikolous, G., Capone, M., Holding, C. and Haynes, D. R. (2004) Factors regulating osteoclast formation in human tissues adjacent to peri-implant bone loss: expression of receptor activator NFkappaB, RANK ligand and osteoprotegerin. Biomaterials. 25, 565-573.   DOI   ScienceOn
6 Dai, M., Zhou, T., Xiong, H., Zou, W., Zhan, P. and Fu, W. (2011) Effect of metal ions Co2+ and Cr3+ on osteoblast apoptosis, cell cycle distribution, and secretion of alkaline phosphatase. Zhongguo. Xiu. Fu. Chong. Jian. Wai. Ke. Za. Zhi. 25, 56-60.
7 Dickens, D. S., Kozielski, R., Khan, J., Forus, A. and Cripe, T. P. (2002) Cyclooxygenase-2 expression in pediatric sarcomas. Pediatr. Dev. Pathol. 5, 356-364.   DOI   ScienceOn
8 Dinarello, C. A. (1993) Blocking interleukin-1 in disease. Blood. Purif. 11, 118-127.   DOI   ScienceOn
9 Fisher, J., McEwen, H. M., Tipper, J. L., Galvin, A. L., Ingram, J., Kamali, A., Stone, M. H. and Ingham, E. (2004) Wear, debris, and biologic activity of cross-linked polyethylene in the knee: benefi ts and potential concerns. Clin. Orthop. Relat. Res. 428, 114-119.   DOI
10 Fleury, C., Petit, A., Mwale, F., Antoniou, J., Zukor, D. J., Tabrizian, M. and Huk, O. L. (2006) Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: morphology, cytotoxicity, and oxidative stress. Biomaterials. 27, 3351-3360.   DOI   ScienceOn
11 Gallo, J., Kaminek, P., Ticha, V., Rihakova, P. and Ditmar, R. (2002) Particle disease. A comprehensive theory of periprosthetic osteolysis: a review. Biomed. Pap. Med. Fac. Univ. Palacky. Olomouc. Czech. Repub. 146, 21-28.   DOI   ScienceOn
12 Ge, Y. C., Liu, P. and Han, X. C. (1997) Effect of ginsenosides Rbl. Rgl. Re and Rhl on HeLa cells in vitro. J. Pharmacology and Clinics of TCM. 13, 18-21.
13 Haynes, D. R., Crotti, T. N., Potter, A. E., Loric, M., Atkins, G. J., Howie, D. W. and Findlay, D. M. (2001) The osteoclastogenic molecules RANKL and RANK are associated with periprosthetic osteolysis. J. Bone. Joint. Surg. Br. 83, 902-911.   DOI   ScienceOn
14 Hu, X. M., Yan, C. K. and Hu, X. M. (2006) Efects of ginsenosides Rgl on apoptosis induced by transient foeal cerebral ischemia in rats. Chin. J. Clinical. Pharm. and. Therapeutics. 11, 192-196.
15 Kimble, R. B., Vannice, J. L., Bloedow, D. C., Thompson, R. C., Hopfer, W., Kung, V. T., Brownfi eld, C. and Pacifi ci, R. (1994) Interleukin-1 receptor antagonist decreases bone loss and bone resorption in ovariectomized rats. J. Clin. Invest. 93, 1959-1967.   DOI   ScienceOn
16 Jacobs, J. J., Roebuck, K. A., Archibeck, M., Hallab, N. J. and Glant, T. T. (2001) Osteolysis: basic science. Clin. Orthop. Relat. Res. 393, 71-77.   DOI
17 Jilka, R. L., Hangoc, G., Girasole, G., Passeri, G., Williams, D. C., Abrams, J. S., Boyce, B., Broxmeyer, H. and Manolagas, S. C. (1992) Increased osteoclast development after estrogen loss: mediation by interleukin-6. Science 257, 88-91.   DOI
18 Kim, Y. S., Kim, D. S. and Kim, S. I. (1998) Ginsenoside Rh2 and Rh3 induce differentiation of HL-60 cells into granulocytes: modulation of protein kinase C isoforms during differentiation by ginsenoside Rh2. Int. J. Biochem. Cell. Biol. 30, 327-338.   DOI   ScienceOn
19 Lahera, V., Goicoechea, M., de Vinuesa, S. G., Miana, M., de las Heras, N., Cachofeiro, V. and Luno, J. (2007) Endothelial dysfunction, oxidative stress and infl ammation in atherosclerosis: beneficial effects of statins. Curr. Med. Chem. 14, 243-248.   DOI   ScienceOn
20 Lei, T. C., Zhu, W.Y. and Xia, M. Y. (2000) Gensenoside Rg1 has sensitizing effect in chernotherapentic treatment of murine melanoma cell line in vitro. Chinese Jouranal of Dermatology. 33, 338-341.
21 Li, C. H. (2005) Gensenoside Rg1 induction of apoptosis HL-60 preliminary study. Guangdong. Medicine. 15, 63-65.
22 LI, Y. N., WU, Y. L. and JIA, Z. H. (2008) The contents and interaction of COX-2 and iNOS in vascular injury of rats with defi ciency of vital energy and efect of ginsenoside. Nat. Prod. Res. Dev. 20, 803-807.
23 Lu, J. P., Ma, Z. C., Yang, J., Huang, J., Wang, S. R. and Wang, S. Q. (2004) Ginsenoside Rg1-induced alterations in gene expression in TNF-alpha stimulated endothelial cells. Chin. Med. J. (Engl). 117, 871-876.
24 Vermes, C., Chandrasekaran, R., Jacobs, J. J., Galante, J. O., Roebuck, K. A. and Glant, T. T. (2001) The effects of particulate wear debris, cytokines, and growth factors on the functions of MG-63 osteoblasts. J. Bone. Joint. Surg. Am. 83-A, 201-211.
25 Mohanty, M. (1996) Cellular basis for failure of joint prosthesis. Biomed. Mater. Eng. 6, 165-172.
26 Pacifi ci, R., Rifas, L., Teitelbaum, S., Slatopolsky, E., McCracken, R., Bergfeld, M., Lee, W., Avioli, L. V. and Peck, W. A. (1987) Spontaneous release of interleukin 1 from human blood monocytes refl ects bone formation in idiopathic osteoporosis. Proc. Natl. Acad. Sci. USA. 84, 4616-4620.   DOI   ScienceOn
27 Pioletti, D. P., Takei, H., Kwon, S. Y., Wood, D. and Sung, K. L. (1999) The cytotoxic effect of titanium particles phagocytosed by osteoblasts. J. Biomed. Mater. Res. 46, 399-407.   DOI   ScienceOn
28 von Knoch, M., Jewison, D. E., Sibonga, J. D., Sprecher, C., Morrey, B. F., Loer, F., Berry, D. J. and Scully, S. P. (2004) The effectiveness of polyethylene versus titanium particles in inducing osteolysis in vivo. J. Orthop. Res. 22, 237-243.   DOI   ScienceOn
29 Wang, Y. K., Zhang, M. L. and Zhang, LB. (2003) Study on ginsenosides induced differentiation of human hepatocellular carcinoma cells. J. Lin. Chuang. Gan. Dan. Bing. Za. Zhi. 19, 242-243.