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http://dx.doi.org/10.4062/biomolther.2011.19.3.331

Anti-Inflammatory Effects of Hexane Fraction from White Rose Flower Extracts via Inhibition of Inflammatory Repertoires  

Lee, Hwa-Jeong (Department of Beauty Design, Dongshin University)
Kim, Han-Seok (Department of Cosmeteology, Chung Am University)
Kim, Seung-Tae (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University)
Park, Dong-Sun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Hong, Jin-Tae (College of Pharmacy, Chungbuk National University)
Kim, Yun-Bae (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Joo, Seong-Soo (Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University)
Publication Information
Biomolecules & Therapeutics / v.19, no.3, 2011 , pp. 331-335 More about this Journal
Abstract
In this study, we determined the anti-inflammatory activity and mechanism of action of a hexane fraction (hWRF) obtained from white Rosa hybrida flowers by employing various assays such as quantitative real-time PCR, Western blotting, and Electrophoretic-Mobility Shift Assay (EMSA). The results revealed that the hWRF had excellent anti-inflammatory potency by reducing inflammatory repertoires, such as inducible nitric oxide synthase (iNOS), interleukin-$1{\beta}$, and cyclooxygenase-2 (COX-2) in RAW264.7 cells when stimulated with lipopolysaccharide (LPS), a pro-inflammatory mediator. The reduction of nitric oxide (NO) release from RAW 264.7 cells supported the anti-inflammatory effect of hWRF. Interestingly, hWRF effectively inhibited LPS-mediated nuclear factor-${\kappa}B$ (NF-${\kappa}B$) p65 subunit translocation into the nucleus and extracellular signal-regulated kinase (ERK)1/2 phosphorylation, suggesting that hWRF anti-inflammatory activity may be based on inhibition of the NF-${\kappa}B$ and MAPK pathways. Based on the findings described in this study, hWRF holds promise for use as a potential anti-inflammatory agent for either therapeutic or functional adjuvant purposes.
Keywords
Rosa hybrida; Inflammatory repertoire; RAW 264.7; Lipopolysaccharide; Nuclear factor-${\kappa}B$;
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