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http://dx.doi.org/10.4062/biomolther.2011.19.2.206

Protein Expression Analysis in Hematopoietic Stem Cells during Osteopontin-Induced Differentiation of Natural Killer Cells  

Kim, Mi-Sun (Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology)
Bae, Kil-Soo (Department of Biological Science, Dong-A University)
Kim, Hye-Jin (Department of Biological Science, Dong-A University)
Yoon, Suk-Ran (Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology)
Oh, Doo-Byung (Aging Research Center, Korea Research Institute of Bioscience and Biotechnology)
Hwang, Kwang-Woo (Department of Immunology, College of Pharmacy, Chung Ang University)
Jun, Woo-Jin (Department of Food and Nutrition, Chonnam National University)
Shim, Sang-In (Department of Agronomy, Gyeongsang National University)
Kim, Kwang-Dong (Division of Applied Life Science (BK21), Gyeongsang National University)
Jung, Yong-Woo (Department of Immunology, College of Pharmacy, Korea University Sejong Campus)
Park, So-Young (Department of Nanobiomedical Science, Dankook University)
Kwon, Ki-Sun (Aging Research Center, Korea Research Institute of Bioscience and Biotechnology)
Choi, In-Pyo (Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology)
Chung, Jin-Woong (Department of Biological Science, Dong-A University)
Publication Information
Biomolecules & Therapeutics / v.19, no.2, 2011 , pp. 206-210 More about this Journal
Abstract
Natural Killer (NK) cells are the lymphocytes that are derived from hematopoietic stem cells, developed in the bone marrow from hematopoietic stem cells (HSC) by sequential acquisition of functional surface receptors, and express the repertoire of inhibitory and activating receptors. Recently, Osteopontin (OPN) has been identified as a critical factor for differentiation of natural killer cells. However, the detailed mechanism of OPN-induced NK differentiation has been still to be elucidated. Here, we determined the signaling pathway and possible receptor for OPN in NK differentiation. OPN induced expression of Bcl-2 and activation of Erk kinase. Inhibition of Erk pathway decreased the effect of OPN on NK differentiation. In addition, the expression of integrin ${\alpha}9$ was significantly increased by OPN during NK differentiation, suggesting the possible role of a major signaling molecule for OPN- induced NK differentiation.
Keywords
Osteopontin; Natural killer cells; Hematopoietic stem cells; Erk; Bcl-2;
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1 Ostiguy, V., Allard, E. L., Marquis, M., Leignadier, J. and Labrecque, N. (2007) IL-21 promotes T lymphocyte survival by activating the phosphatidylinositol-3 kinase signaling cascade. J. Leukoc. Biol. 82, 645-656.   DOI
2 Perussia, B. (1996) The cytokine profile of resting and activated NK cells. Methods 9, 370-378.   DOI
3 Robertson, B. W., Bonsal, L. and Chellaiah, M. A. (2010) Regulation of Erk1/2 activation by osteopontin in PC3 human prostate cancer cells, Molecular Cancer 9, 260.   DOI
4 Schack, L., Stapulionis, R., Christensen, B., Kofod-Olsen, E., Skov Sorensen, U. B., Vorup-Jensen, T., Sorensen, E. S. and Hollsberg, P. (2009) Osteopontin enhances phagocytosis through a novel osteopontin receptor, the alphaXbeta2 integrin, J Immunol. 182, 6943-6950.   DOI
5 Sheridan, C., Brumatti, G., Elgendy, M., Brunet, M. and Martin, S. J. (2010) An ERK-dependent pathway to Noxa expression regulates apoptosis by platinum-based chemotherapeutic drugs. Oncogene. 29, 6428-6441.   DOI
6 Stier, S., Ko, Y., Forkert, R., Lutz, C., Neuhaus, T., Grunewald, E., Cheng, T., Dombkowski, D., Calvi, L. M., Rittling, S. R. and Scadden D. T. (2005) Osteopontin is a hematopoietic stem cell niche component that negatively regulates stem cell pool size. J. Exp. Med. 201, 1781-1791.   DOI
7 Veronique Orian-Rousseau. (2010) CD44, a therapeutic target for metastasising tumours. Eur. J. Cancer 46, 1271-1277.   DOI
8 Bonanno, G., Mariotti, A., Procoli, A., Corallo, M., Scambia, G., Pierelli, L. and Rutella, S. (2009) Interleukin-21 induces the differentiation of human umbilical cord blood CD34-lineage- cells into pseudomature lytic NK cells. BMC Immunol. 10, 46.   DOI
9 Chung, J. W., Kim, M. S., Piao, Z. H., Jeong, M., Yoon, S. R., Shin, N., Kim, S. Y., Hwang, E. S., Yang, Y., Lee, Y. H., Kim, Y. S. and Choi, I. (2008) Osteopontin promotes the development of natural killer cells from hematopoietic precursor cells. Stem Cells 26, 2114-2123.   DOI
10 Di Santo, J. P. (2006) Natural killer cell developmental pathways: a question of balance. Annu. Rev. Immunol. 24, 257-286.   DOI
11 El-Tanani, M. K. (2008) Role of osteopontin in cellular signaling and metastatic phenotype. Front Biosci. 1, 4276-4284.
12 Freud, A. G. and Caligiuri, M. A. (2006) Human natural killer cell development. Immunol. Rev. 214, 56-72.   DOI
13 Mauchera, C., Weissingerb, E. M., Kremmerc, E., Baccarinind, M., Procykd, K., Hendersone, D. W., Wolffe, L., Kolcha, W., Kaspersf, B., Mushinskie, J. F. and Mischaka H. (1998) Activation of bcl-2 suppressible 40 and 44 kDa p38-like kinases during apoptosis of early and late B lymphocytic cell lines. FEBS Letter. 427, 29-35.   DOI
14 Orian-Rousseau, V. (2010) CD44, a therapeutic target for metastasising tumours. Eur. J. Cancer 46, 1271-1277.   DOI
15 Metge, B. J., Liu, S., Riker, A. I., Fodstad, O., Samant, R. S. and Shevde, L. A. (2010) Elevated osteopontin levels in metastatic melanoma correlate with epigenetic silencing of breast cancer metastasis suppressor 1. Oncology 78, 75-86.   DOI
16 Nilsson, S. K., Johnston, H. M., Whitty, G. A., Williams, B, Webb, R. J., Denhardt, D. T., Bertoncello, I, Bendall, L. J., Simmons, P. J. and Haylock, D. N. (2005) Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells. Blood 106, 1232-1239.   DOI   ScienceOn