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http://dx.doi.org/10.4062/biomolther.2011.19.1.059

Evaluation of Immunotoxicity of Shizukaol B Isolated from Chloranthus japonicus  

Kwon, Soon-Woo (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Kim, Young-Kook (Korea Research Institute of Bioscience and Biotechnology)
Kim, Jee-Youn (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Ryu, Hwa-Sun (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Lee, Hong-Kyung (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Kang, Jong-Soon (Korea Research Institute of Bioscience and Biotechnology)
Kim, Hwan-Mook (Korea Research Institute of Bioscience and Biotechnology)
Hong, Jin-Tae (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Kim, Young-Soo (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Han, Sang-Bae (College of Pharmacy and Medical Research Center (CICT), Chungbuk National University)
Publication Information
Biomolecules & Therapeutics / v.19, no.1, 2011 , pp. 59-64 More about this Journal
Abstract
Dimeric sesquiterpenoid shizukaol B (SKB) was isolated from Chloranthus japonicus Sieb. Except that SKB inhibited adhesion molecule expression in monocytes and endothelial cells, no more biological and pharmacological activity of SKB had been reported until now. In this study, we examined immunosuppressive activity of SKB. SKB strongly inhibited lipopolysaccharide (LPS)-induced B cell proliferation with $IC_{50}$ of 137 ng/ml, but slightly or not concanavalin A-induced T cell proliferation, LPS-induced macrophage NO production, and LPS-induced dendritic cell maturation. As a mechanism, SKB strongly induced apoptotic death of B cells, but not other cell types. These results suggested that SKB induced toxicity-mediated immunosuppression against B cells.
Keywords
Shizukaol B; Chloranthus japonicus; Immunosuppression; Cytotoxicity; B cells;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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1 Kuang, H. X., Xia, Y. G., Yang, B. Y., Wang, Q. H. and Lu, S. W. (2009) Lignan constituents from Chloranthus japonicus sieb. Arch. Pharm. Res. 32, 329-334.   DOI
2 Kwon, O. E., Lee, H. S., Lee, S. W., Bae, K., Kim, K., Hayashi, M., Rho, M. C. and Kim, Y. K. (2006) Dimeric sesquiterpenoids isolated from Chloranthus japonicus inhibited the expression of cell adhesion molecules. J. Ethnopharmacol. 104, 270-277.   DOI
3 Nagahara, Y., Ikekita, M. and Shinomiya, T. (2002) T cell selective apoptosis by a novel immunosuppressant, FTY720, is closely regulated with Bcl-2. Br. J. Pharmacol. 137, 953-962.   DOI
4 Nagarkatti, M., Rieder, S. A., Vakharia, D. and Nagarkatti, P. S. (2010) Evaluation of apoptosis in immunotoxicity testing. Methods. Mol. Biol. 598, 241-257.   DOI
5 Pallardy, M., Biola, A., Lebrec, H. and Breard, J. (1999) Assessment of apoptosis in xenobiotic-induced immunotoxicity. Methods 19, 36-47.   DOI
6 Schutz, C., Fischer, K., Volkl, S., Hoves, S., Halbritter, D., Mackensen, A. and Fleck, M. (2009) A new flow cytometric assay for the simultaneous analysis of antigen-specific elimination of T cells in heterogeneous T cell populations. J. Immunol. Methods 344, 98-108.   DOI
7 Shi, G. X., Harrison, K., Han, S. B., Moratz, C. and Kehrl, J. H. (2004) Toll-like receptor signaling alters the expression of regulator of G protein signaling proteins in dendritic cells: implications for G protein-coupled receptor signaling. J. Immunol. 172, 5175-5184.   DOI
8 Williams, O. (2004) Flow cytometry-based methods for apoptosis detection in lymphoid cells. Methods Mol. Biol. 282, 31-42.
9 Duffin, R., Leitch, A. E., Fox, S., Haslett, C. and Rossi, A. G. (2010) Targeting granulocyte apoptosis: mechanisms, models, and therapies. Immunol. Rev. 236, 28-40.   DOI
10 Han, S. B., Kim, H. M., Kim, Y. H., Lee, C. W., Jang, E. S., Son, K. H., Kim, S. U. and Kim, Y. K. (1998) T-cell specific immunosuppression by prodigiosin isolated from Serratia marcescens. Int. J. Immunopharmacol. 20, 1-13.   DOI
11 Han, S. B., Lee, C. W., Yoon, Y. D., Lee, J. H., Kang, J. S., Lee, K. H., Yoon, W. K., Lee, K., Park, S. K. and Kim, H. M. (2005a) Prevention of arthritic inflammation using an oriental herbal combination BDX-1 isolated from Achyranthes bidentata and Atractylodes japonica. Arch. Pharm. Res. 28, 902-908.   DOI
12 Han, S. B., Moratz, C., Huang, N. N., Kelsall, B., Cho, H., Shi, C. S., Schwartz, O. and Kehrl, J. H. (2005b) Rgs1 and Gnai2 regulate the entrance of B lymphocytes into lymph nodes and B cell motility within lymph node follicles. Immunity 22, 343-354.   DOI
13 Kuang, H., Xia, Y., Yang, B., Wang, Q. and Lu, S. (2008) Sesquiterpene glucosides from Chloranthus japonicus sieb. Chem. Biodivers 5, 1736-1742.   DOI
14 Han, S. B., Park, S. H., Jeon, Y. J., Kim, Y. K., Kim, H. M. and Yang, K. H. (2001) Prodigiosin blocks T cell activation by inhibiting interleukin-2Ralpha expression and delays progression of autoimmune diabetes and collagen-induced arthritis. J. Pharmacol. Exp. Ther. 299, 415-425.
15 Kim, J. Y., Kang, J. S., Kim, H. M., Kim, Y. K., Lee, H. K., Song, S., Hong, J. T., Kim, Y. and Han, S. B. (2009) Inhibition of phenotypic and functional maturation of dendritic cells by manassantin a. J. Pharmacol. Sci. 109, 583-592.   DOI
16 Kim, J. Y., Yoon, Y. D., Ahn, J. M., Kang, J. S., Park, S. K., Lee, K., Song, K. B., Kim, H. M. and Han, S. B. (2007) Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4. Int. Immunopharmacol. 7, 78-87.   DOI
17 Basten, A. (2010) The role of B cells in transplantation and immunopathic diseases. Immune. Netw. 10, 81-84.   DOI