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http://dx.doi.org/10.4062/biomolther.2009.17.4.412

Antioxidative Constituents from the Twigs of Vitex rotundifolia  

Kim, Dae-Keun (College of Pharmacy, Woosuk University)
Publication Information
Biomolecules & Therapeutics / v.17, no.4, 2009 , pp. 412-417 More about this Journal
Abstract
In the course of screening for antioxidant compounds by measuring the radical scavenging effect on DPPH (1,1-diphenyl- 2-picrylhydrazyl), a total extract of the twigs of Vitex rotundifolia (Verbenaceae) was found to show potent antioxidant activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of three iridoid compounds, 10-O-vanilloylaucubin (1), 10-O-p-hydroxybenzoylaucubin (2) and aucubin (3), two C-glycoside flavones, vitexin (4) and orientin (5), and a quinic acid derivative, 3,4-di-O-caffeoylquinic acid (6). Their structures were elucidated by spectroscopic studies. Among them, compounds 5 and 6 showed the significant antioxidative effects on DPPH free radical scavenging test. In riboflavin-NBT-light and xanthine-NBT-xanthine oxidase systems, compounds 5 and 6 exhibited the formation of the blue formazan in a dose-dependent manner. Compounds 5 and 6 showed better superoxide quenching activities than vitamin C.
Keywords
Vitex rotundifolia; Verbenaceae; Iridoid; Phenolic compounds; DPPH; Superoxide quenching activity;
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