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http://dx.doi.org/10.4062/biomolther.2009.17.3.263

Evaluation on Pharmacological Activities of 2,4-Dihydroxybenzaldehyde  

Jung, Hyun-Joo (Brain Korea 21 Project for Medical Science, Department of Anatomy, Yonsei University College of Medicine)
Song, Yun-Seon (College of Pharmacy, Sookmyung Women's University)
Lim, Chang-Jin (Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University)
Park, Eun-Hee (College of Pharmacy, Sookmyung Women's University)
Publication Information
Biomolecules & Therapeutics / v.17, no.3, 2009 , pp. 263-269 More about this Journal
Abstract
4-Hydroxybenzaldehyde, a phenolic compound found in a variety of natural sources, was previously shown to contain anti-inflammatory and related anti-angiogenic and anti-nociceptive activities. The present work was designed to assess some pharmacological activities of 2,4-dihydroxybenzaldehyde (DHD), an analogue of 4-hydroxybenzaldehyde. DHD exhibited a significant inhibition in the chick chorioallantoic membrane (CAM) angiogenesis, and its $IC_{50}$ value was $2.4\;{\mu}g/egg$. DHD also contained in vivo anti-inflammatory activity using acetic acid-induced permeability and carrageenan-induced air pouch models in mice. In the air pouch model, DHD showed significant suppression in exudate volume, number of polymorphonuclear leukocytes and nitrite content. DHD showed an anti-nociceptive activity in the acetic acid-induced writhing test in mice. It also suppressed enhanced production of nitric oxide (NO) and elevated expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. It was able to slightly decrease the level of reactive oxygen species in the stimulated macrophages. DHD at the used concentrations couldn't modulate the viabilities of RAW264.7 cells. Taken together, like 4-hydroxybenzaldehyde, DHD contains anti-angiogenic, anti-inflammatory and anti-nociceptive activities.
Keywords
Anti-angiogenic; Anti-inflammatory; Anti-nociceptive; Cyclooxygenase-2; 2,4-dihydroxybenzaldehyde; Inducible nitric oxide synthase; Nitric oxide; RAW264.7; Reactive oxygen species;
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