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http://dx.doi.org/10.4062/biomolther.2009.17.2.211

Metabolism of Wogonoside by Human Fecal Microflora and Its Anti-pruritic Effect  

Trinh, Hien-Trung (Department of Pharmaceutical Science and Department of Life and Pharmaceutical Sciences, Kyung Hee University)
Jang, Seo-Young (Department of Food and Nutrition, Kyung Hee University)
Han, Myung-Joo (Department of Food and Nutrition, Kyung Hee University)
Kawk, Ho-Young (Graduate School of Biotechnology and PMRC, Kyung Hee University)
Baek, Nam-In (Graduate School of Biotechnology and PMRC, Kyung Hee University)
Kim, Dong-Hyun (Department of Pharmaceutical Science and Department of Life and Pharmaceutical Sciences, Kyung Hee University)
Publication Information
Biomolecules & Therapeutics / v.17, no.2, 2009 , pp. 211-216 More about this Journal
Abstract
To understand the relationship between the metabolism of wogonoside from the rhizome of Scutellaria baicalensis, and its anti-pruritic effect, we anaerobically incubated it with human fecal microflora, identified its metabolite identified, and investigated its anti-pruritic effect in compound 48/80 or histamineinduced pruritic mice. Wogonoside was metabolized to wogonin, with metabolic activity of $6.9{\pm}5.1\;nmol/h/mg$ wet weight of fecal microflora. Orally administered wogonoside had more potent anti-scratching behavioral effect in compound 48/80 or histamine-treated mice than intraperitoneally treated one, apart from orally administered its metabolite, wogonin, which was more potent than the orally administered one. Wogonoside showed more potent anti-pruritic effects when administered at 5 h prior to the pruritic agent treatment than when administered at 1 h before. However, wogonin orally administered 1 h before the treatment with pruritic agents showed a more potent anti-pruritic effect than when treated at 5 h before. Orally administered wogonoside may be metabolized to wogonin in the intestine and its anti-scratching behavioral effect may be dependent on its metabolism by intestinal microflora.
Keywords
Scutellaria baicalensis; wogonoside; wogonin; pruritus; metabolism; intestinal microflora;
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