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http://dx.doi.org/10.4062/biomolther.2009.17.2.188

In Vivo Suppression of Bisphenol A on Estradiol 2- and 4-Hydroxylase Activities in Hepatic Microsomal Fractions of Male and Female Sprague-Dawley Rats  

Nugraha, Boya (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
Yoon, Ae-Rin (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
Kandagaddala, Lakshmi Devi (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
Cho, Hyo-Joo (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
Chung, Bong-Chul (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
Kwon, Oh-Seung (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
Publication Information
Biomolecules & Therapeutics / v.17, no.2, 2009 , pp. 188-198 More about this Journal
Abstract
This work was conducted to investigate the effect of bisphenol A (BPA) on estradiol (E2) 2-and 4-hydroxylase activities in the liver, kidney and lung tissues of male and female rats. After intraperitoneal administration of BPA to male and female rats for 4 days at 0, 10, and 50 mg/kg, the conversion of the substrate for hepatic and extra-hepatic enzyme activities was measured by GC/MSD. The result showed decreases of body and organ weights at 50 mg/kg BPA of male and female rats. Male hepatic E2 2-hydroxylase activity was inhibited by 68% at 10 mg/kg and by 82% at 50 mg/kg BPA. Female hepatic E2 2-hydroxylase activity was decreased by 46% at 10 mg/kg and by 56% at 50 mg/kg to the control. E2 4-hydroxylase was inhibited by 57 and 57% at 10 mg/kg and 54 and 78% at 50 mg/kg in liver of female and male, respectively. The urinary excretion rate of 2-hydroxyestradiol (2-OHE), androsterone and testosterone in urine of female rats with 50 mg/kg BPA were decreased significantly. The results showed that 50 mg/kg BPA was decreased E2 2-and 4-hydroxylase activities in liver, but not in other tissues. The urinary excretion rates of 2-OHE, androsterone and testosterone were also decreased. In liver, estrogenic enzyme activity were higher in male than female. These results suggest that BPA can disrupt estrogen metabolism by suppressing E2 2-and 4-hydroxylase activities in the liver of male and female rats.
Keywords
Bisphenol A; Estradiol 2-hydroxylase; Estradiol 4-hydroxylase; Estrogen metabolites; Rat;
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