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http://dx.doi.org/10.4062/biomolther.2009.17.1.12

Identification of Regulatory Role of KRAB Zinc Finger Protein ZNF 350 and Enolase-1 in RE-IIBP Mediated Transcriptional Repression  

Kim, Ji-Young (Department of Life Science, College of Natural Sciences, Chung-Ang University)
Seo, Sang-Beom (Department of Life Science, College of Natural Sciences, Chung-Ang University)
Publication Information
Biomolecules & Therapeutics / v.17, no.1, 2009 , pp. 12-16 More about this Journal
Abstract
One of the WHSC1/MMSET/NSD2 variant RE-IIBP is a histone H3-K27 methyltransferase with transcriptional repression activity. Overexpression of RE-IIBP in various types of leukemia suggests it's role in leukemogenesis. Here we identify two proteins, KRAB zinc finger protein ZNF 350 and enolase-1 as RE-IIBP interacting proteins by yeast two-hybrid screening and confirmed direct interaction in vivo and in vitro. Both proteins have been known for their role in transcriptional repression. Reporter assays using transient transfection demonstrated that both ZNF 350 and enolase-1 proteins synergistically repressed transcription with RE-IIBP, respectively. These results indicate both proteins have roles in RE-IIBP mediated transcriptional repression by involving co-repressor complex.
Keywords
RE-IIBP; Yeast two-hybrid screening; ZNF 350; Enolase-1;
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1 Feo S., Arcuri D., Piddini E., Passantino R. and Giallongo A. (2000). ENO1 gene product binds to the c-myc promoter and acts as a transcriptional repressor: relationship with Myc promoter-binding protein 1 (MBP-1). FEBS. Lett. 473, 47-52   DOI   ScienceOn
2 Garlisi C. G., Uss A. S., Xiao H., Tian F., Sheridan K. E., Wang L., Motasim Billah M., Egan R. W., Stranick K. S. and Umland S. P. (2001). A unique mRNA initiated within a middle intron of WHSC1/MMSET encodes a DNA binding protein that suppresses human IL-5 transcription. Am. J. Respir. Cell. Mol. Biol. 24, 90-98   DOI   ScienceOn
3 Tan W., Zheng L., Lee W. H. and Boyer T. G. (2004). Functional dissection of transcription factor ZBRK1 reveals zinc fingers with dual roles in DNA-binding and BRCA1-dependent transcriptional repression. J. Biol. Chem. 279, 6576-6587   DOI   ScienceOn
4 Vissing H., Meyer W. K., Aagaard L., Tommerup N. and Thiesen H. J. (1995). Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins. FEBS. Lett. 369, 153-157   DOI   ScienceOn
5 Ray R. and Miller D. M. (1991). Cloning and characterization of a human c-myc promoter-binding protein. Mol. Cell Biol. 11, 2154-2161   DOI
6 Ito T., Ota K., Kubota H., Yamaguchi Y., Chiba T., Sakuraba K. and Yoshida M. (2002). Roles for the two-hybrid system in exploration of the yeast protein interactome. Mol. Cell Proteomics. 1, 561-566   DOI
7 Kouzarides T. (2007). Chromatin modifications and their function. Cell 128, 693-705   DOI   ScienceOn
8 Peng H., Zheng L., Lee W. H., Rux J. J. and Rauscher F. J. 3rd. (2002). A common DNA-binding site for SZF1 and the BRCA1-associated zinc finger protein, ZBRK1. Cancer Res. 62, 3773-3781
9 Schultz D. C., Ayyanathan K., Negorev D., Maul G. G. and Rauscher F. J. 3rd. (2002). SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Genes Dev. 16, 919-932   DOI   ScienceOn
10 Sims R. J. 3rd., Nishioka K. and Reinberg D. (2003). Histone lysine methylation: a signature for chromatin function. Trends. Genet. 19, 629-639   DOI   ScienceOn
11 Ghosh A. K., Steele R. and Ray R. B. (2005). c-myc Promoterbinding protein 1 (MBP-1) regulates prostate cancer cell growth by inhibiting MAPK pathway. J. Biol. Chem. 280, 14325-14330   DOI   ScienceOn
12 Subramanian A. and Miller D. M. (2000). Structural analysis of alpha-enolase. Mapping the functional domains involved in down-regulation of the c-myc protooncogene. J. Biol. Chem. 275, 5958-5965   DOI   ScienceOn
13 Kim J. Y., Kee H. J., Choe N. W., Kim S. M., Eom G. H., Baek H. J., Kook H., Kook H. and Seo S. B. (2008). Multiple-myelomarelated WHSC1/MMSET isoform RE-IIBP is a histone methyltransferase with transcriptional repression activity. Mol. Cell. Biol. 28, 2023-2034   DOI   ScienceOn
14 Witzgall R., O'Leary E., Leaf A., Onaldi D. and Bonventre J. V. (1994). The Kruppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proc. Natl. Acad. Sci. U S A. 91, 4514-4518   DOI
15 Ejeskar K., Krona C., Caren H., Zaibak F., Li L., Martinsson T. and Ioannou P. A. (2005). Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC. Cancer 5, 161   DOI