Browse > Article
http://dx.doi.org/10.4062/biomolther.2008.16.4.370

Inhibitory Effect of Ginsenoside-Rp1, a Novel Ginsenoside Derivative, on the Functional Activation of Macrophage-like Cells  

Park, Tae-Yoon (CAmbo Institute)
Cho, Jae-Youl (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Biomolecules & Therapeutics / v.16, no.4, 2008 , pp. 370-376 More about this Journal
Abstract
Ginsenoside Rp1 (G-Rp1) is a ginseng saponin derivative with chemopreventive and anti-cancer activities. In this study, we examined the regulatory activity of G-Rp1 on the functional activation of macrophages. G-Rp1 remarkably inhibited TNF-$\alpha$ production, LPS-induced cell cytotoxicity, NO production, ROS generation, and phagocytic uptake from lipopolysacchride (LPS)-activated RAW264.7 cells. According to structural feature study using several G-Rp1 analogs, two carbohydrates (glucose-glucose) at R1 position were observedto be highly effective, compared to other structural derivatives. Although the inhibitory activities of G-Rp1 on macrophage functions were not remarkable, several points that G-Rp1 was known to be safe, and that this compound was orally effective, suggest that G-Rp1 may be beneficial in treating macrophage-mediated immunological diseases.
Keywords
Ginseng saponin; Macrophage function; RAW264.7 cells; Immunological disease; Inflammation;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Ding, M., Zhang, M., Wong, J. L., Rogers, N. E., Ignarro, L. J. and Voskuhl, R. R. (1998). Antisense knockdown of inducible nitric oxide synthase inhibits induction of experimental autoimmune encephalomyelitis in SJL/J mice. J. Immunol. 160, 2560-2564
2 Haberzettl, P., Duffin, R., Kramer, U., Hohr, D., Schins, R. P., Borm, P. J. and Albrecht, C. (2007). Actin plays a crucial role in the phagocytosis and biological response to respirable quartz particles in macrophages. Arch. Toxicol. 81, 459-470   DOI
3 Cho, J. Y., Yoo, E. S., Baik, K. U., Park, M. H. and Han, B. H. (2001b). In vitro inhibitory effect of protopanaxadiol ginsenosides on tumor necrosis factor (TNF)-alpha production and its modulation by known TNF-alpha antagonists. Planta. Med. 67, 213-218   DOI   ScienceOn
4 Choo, M. K., Sakurai, H., Kim, D. H. and Saiki, I. (2008). A ginseng saponin metabolite suppresses tumor necrosis factoralpha-promoted metastasis by suppressing nuclear factorkappaB signaling in murine colon cancer cells. Oncol. Rep. 19, 595-600
5 Kumar, A., Kumar, M., Panwar, M., Samarth, R. M., Park, T. Y., Park, M. H. and Kimura, H. (2006). Evaluation of chemopreventive action of Ginsenoside Rp1. Biofactors 26, 29-43   DOI
6 Bai, X. C., Lu, D., Liu, A. L., Zhang, Z. M., Li, X. M., Zou, Z. P., Zeng, W. S., Cheng, B. L. and Luo, S. Q. (2005). Reactive oxygen species stimulates receptor activator of NF-kappaB ligand expression in osteoblast. J. Biol. Chem. 280, 17497-17506   DOI   ScienceOn
7 Cho, J. Y., Baik, K. U., Jung, J. H. and Park, M. H. (2000). In vitro anti-inflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa. Eur. J. Pharmacol. 398, 399-407   DOI   ScienceOn
8 Wang, E., Michl, J., Pfeffer, L. M., Silverstein, S. C. and Tamm, I. (1984). Interferon suppresses pinocytosis but stimulates phagocytosis in mouse peritoneal macrophages: related changes in cytoskeletal organization. J. Cell. Biol. 98, 1328-1341   DOI
9 Choi, K., Kim, M., Ryu, J. and Choi, C. (2007). Ginsenosides compound K and Rh(2) inhibit tumor necrosis factor-alphainduced activation of the NF-kappaB and JNK pathways in human astroglial cells. Neurosci. Lett. 421, 37-41   DOI   ScienceOn
10 Gillis, C. N. (1997). Panax ginseng pharmacology: a nitric oxide link? Biochem. Pharmacol. 54, 1-8   DOI   ScienceOn
11 Lee, H. J., Hyun, E. A., Yoon, W. J., Kim, B. H., Rhee, M. H., Kang, H. K., Cho, J. Y. and Yoo, E. S. (2006), In vitro antiinflammatory and anti-oxidative effects of Cinnamomum camphora extracts. J. Ethnopharmacol. 103, 208-216   DOI   ScienceOn
12 Gracie, J. A., Forsey, R. J., Chan, W. L., Gilmour, A., Leung, B. P., Greer, M. R., Kennedy, K., Carter, R., Wei, X. Q., Xu, D., Field, M., Foulis, A., Liew, F. Y. and McInnes, I. B. (1999). A proinflammatory role for IL-18 in rheumatoid arthritis. J. Clin. Invest. 104, 1393-1401   DOI   ScienceOn
13 Hong, S., Kim, S. H., Rhee, M. H., Kim, A. R., Jung, J. H., Chun, T., Yoo, E. S. and Cho, J. Y. (2003). In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges. Naunyn Schmiedebergs Arch. Pharmacol. 368, 448-456   DOI
14 Jung, C. H., Jun, C. Y., Lee, S., Park, C. H., Cho, K. and Ko, S. G. (2006). Rhus verniciflua stokes extract: radical scavenging activities and protective effects on H2O2-induced cytotoxicity in macrophage RAW 264.7 cell lines. Biol. Pharm. Bull. 29, 1603-1607   DOI   ScienceOn
15 Lee, J. Y., Rhee, M. H. and Cho, J. Y. (2008a). Novel modulatory effects of SDZ 62-434 on inflammatory events in activated macrophage-like and monocytic cells. Naunyn Schmiedebergs Arch. Pharmacol. 377, 111-124   DOI
16 Lee, Y. G., Lee, W. M., Kim, J. Y., Lee, J. Y., Lee, I. K., Yun, B. S., Rhee, M. H. and Cho, J. Y. (2008b). Src kinase-targeted anti-inflammatory activity of davallialactone from Inonotus xeranticus in lipopolysaccharide-activated RAW264.7 cells. Br. J. Pharmacol. 154, 852-863   DOI   ScienceOn
17 Vandana, S., Ram, S., Ilavazhagan, M., Kumar, G. D. and Banerjee, P. K. (2006). Comparative cytoprotective activity of vitamin C, E and beta-carotene against chromium induced oxidative stress in murine macrophages. Biomed. Pharmacother. 60, 71-76   DOI   ScienceOn
18 Michaelsson, E., Holmdahl, M., Engstrom, A., Burkhardt, H., Scheynius, A. and Holmdahl, R. (1995). Macrophages, but not dendritic cells, present collagen to T cells. Eur. J. Immunol. 25, 2234-2241   DOI   ScienceOn
19 Miller, Y. I., Worrall, D. S., Funk, C. D., Feramisco, J. R. and Witztum, J. L. (2003). Actin polymerization in macrophages in response to oxidized LDL and apoptotic cells: role of 12/15-lipoxygenase and phosphoinositide 3-kinase. Mol. Biol. Cell. 14, 4196-4206   DOI   ScienceOn
20 Plenz, G. and Robenek, H. (1998). Monocytes/macrophages in atherosclerosis. Eur. Cytokine. Netw. 9, 701-703
21 Shin, Y. W., Bae, E. A., Kim, S. S., Lee, Y. C., Lee, B. Y., and Kim, D. H. (2006). The effects of ginsenoside Re and its metabolite, ginsenoside Rh1, on 12-O-tetradecanoylphorbol 13-acetate- and oxazolone-induced mouse dermatitis models. Planta Med. 72, 376-378   DOI   ScienceOn
22 Kinne, R. W., Brauer, R., Stuhlmuller, B., Palombo-Kinne, E. and Burmester, G. R. (2000). Macrophages in rheumatoid arthritis. Arthritis Res. 2, 189-202   DOI   ScienceOn
23 Bae, E. A., Kim, E. J., Park, J. S., Kim, H. S., Ryu, J. H., and Kim, D. H. (2006). Ginsenosides Rg3 and Rh2 inhibit the activation of AP-1 and protein kinase A pathway in lipopolysaccharide/interferon-gamma-stimulated BV-2 microglial cells. Planta Med. 72, 627-633   DOI   ScienceOn
24 Cho, J. Y., Fox, D. A., Horejsi, V., Sagawa, K., Skubitz, K. M., Katz, D. R. and Chain, B. (2001a). The functional interactions between CD98, ${\beta}1$-integrins, and CD147 in the induction of U937 homotypic aggregation. Blood 98, 374-382   DOI   ScienceOn
25 Lee, Y. G., Kim, J. Y., Lee, J. Y., Byeon, S. E., Hong, E. K., Lee, J., Rhee, M. H., Park, H. J. and Cho, J. Y. (2007). Regulatory effects of Codonopsis lanceolata on macrophage-mediated immune responses. J. Ethnopharmacol. 112, 180-188   DOI   ScienceOn
26 Park M. H. and Park T. Y. (2005). Novel ginsenoside preparation with anti-cancer activity. PCT/KR2005/117501