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http://dx.doi.org/10.4062/biomolther.2008.16.1.046

Hypolipidemic and Hepatoprotecitive Effects of Picrorrhiza Rhizoma in High Fat Diet Supplied Mice. A Pevention Sudy.  

Lee, Hyeung-Sik (Department of Herbal Biotechnology, Daegu Haany University)
Woo, Sung-Jung (Department of Herbal Biotechnology, Daegu Haany University)
Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, Development Team for The New Drug of Oriental Medicine (BK21 program), Daegu Haany University)
Publication Information
Biomolecules & Therapeutics / v.16, no.1, 2008 , pp. 46-53 More about this Journal
Abstract
The preventive hypolipidemic effect of the aqueous extracts of Picrorrhiza Rhizoma (PR) was observed in a high fat diet (HFD) feeding hyperlipidemic mouse with their hepatoprotective effects. PR extracts (50, 100 and 200mg/kg) were orally dosed once a day for 12 weeks initiated with HFD supply, and changes on body weight and gains, liver weight, serum aspartate transferase (AST) and alanine transferase (ALT) levels were monitored with serum low density lipoprotein (LDL), high density lipoprotein (HDL), triglyceride and total cholesterol levels. The efficacy of test articles was compared to that of 10mg/kg of simvastatin (SIMVA). Dramatic decrease of both absolute and relative liver weight was dose-dependently observed in all PR extract dosing groups as compared with HFD control group. The serum AST and ALT levels were dose-dependently decreased in PR extract dosing groups. The serum LDL, triglyceride and total cholesterol levels were dose-dependently decreased in PR extract dosing groups compared to that of HFD control group. The serum HDL levels were slightly but dose-dependently increased in PR extract dosing groups as compared with control group. The efficacy on the serum lipid levels of PR extracts was slighter than that of SIMVA. Based on these results, it is concluded that water extract of PR has a relatively good favorable preventive effects on the HFD inducing hyperlipidemia and hepatopathy.
Keywords
Picrorrhiza Rhizoma; extracts; high fat diet; hyperlipidemia; effect; liver;
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1 Joy, K.L. and Kuttan, R. (1999). Anti-diabetic activity of Picrorrhiza kurroa extract. J. Ethnopharmacol. 67, 143- 148   DOI   ScienceOn
2 Yokode, M., Hammer, R.E., Ishibashi, S., Brown, M.S. and Goldstein, J.L. (1990). Diet-induced hypercholesterolemia in mice: prevention by overexpression of LDL receptors. Science, 50, 1273-1275
3 Bhandari, U., Kanojia, R. and Pillai, K.K. (2002). Effect of ethanolic extract of Embelia ribes on dyslipidemia in diabetic rats. Int. J. Exp. Diabetes Res. 3, 159-162   DOI
4 Ble-Castillo, J.L., Rodriguez-Hernandez, A., Miranda-Zamora, R., Juarez-Oropeza, M.A. and Diaz-Zagoya, J.C. (2002). Arthrospira maxima prevents the acute fatty liver induced by the administration of simvastatin, ethanol and a hypercholesterolemic diet to mice. Life Sci. 70, 2665-2673   DOI   ScienceOn
5 Forrester, J.S., Makkar, R. and Shah, P.K. (2005). Increasing high-density lipoprotein cholesterol in dyslipidemia by cholesteryl ester transfer protein inhibition: an update for clinicians. Circulation, 111, 1847-1854   DOI   ScienceOn
6 Cheng, J.W. (2004) Rosuvastatin in the management of hyperlipidemia. Clin. Ther. 26, 1368-1387   DOI   ScienceOn
7 Grundy, S.M. (1986). Cholesterol and coronary heart disease: a new era. J. Am. Med. Assoc. 256, 2849-2858   DOI
8 Han, L.K., Zheng, Y.N., Yoshikawa, M., Okuda, H. and Kimura, Y. (2005). Anti-obesity effects of chikusetsusaponins isolated from Panax japonicus rhizomes. BMC Complement Altern. Med. 5, 9   DOI   ScienceOn
9 Ko, Y.J., Wu, Y.W. and Lin, W.C. (2004). Hypolipidemic effect of Glycine tomentella root extract in hamsters. Am. J. Chin. Med. 32, 57-63   DOI   ScienceOn
10 Lal, J.J., Sreeranjit Kumar, C.V., Suresh, M.V., Indira, M. and Vijayammal, P.L. (1998). Effect of in utero exposure of Toddy (coconut palm wine) on liver function and lipid metabolism in rat fetuses. Plant Foods Hum. Nutr. 52, 209-219   DOI   ScienceOn
11 Dimo, T., Rakotonirina, S.V., Tan, P.V., Azay, J., Dongo, E. and Cros, G. (2002). Leaf methanol extract of Bidens pilosa prevents and attenuates the hypertension induced by high-fructose diet in Wistar rats. J. Ethnopharmacol. 83, 183-191   DOI   ScienceOn
12 el-Saadany, S.S., el-Massry, R.A., Labib, S.M. and Sitohy, M.Z. (1991). The biochemical role and hypocholesterolaemic potential of the legume Cassia fistula in hypercholesterolaemic rats. Nahrung 35, 807-815   DOI   ScienceOn
13 Movahedian, A., Ghannadi, A., Sadeghi, H., Gharavi, M. and Azarpajhoh, S. (2004). Study of leek (Allium porrum. L) extract on cholesterol plasma levels in hyperlipidemic animals. Asia Pac. J. Clin. Nutr. 13, S103
14 Mukai, M., Ozasa, K., Hayashi, K. and Kawai, K. (2002). Various S-GOT/S-GPT ratios in nonviral liver disorders and related physicalconditions and life-style. Dig. Dis. Sci. 47, 549-555   DOI   ScienceOn
15 Herrera-Arellano, A., Aguilar-Santamaria, L., Garcia-Hernandez, B., Nicasio-Torres, P. and Tortoriello, J. (2004). Clinical trial of Cecropia obtusifolia and Marrubium vulgare leaf extracts on blood glucose and serum lipids in type 2 diabetics. Phytomedicine 11, 561-566   DOI   ScienceOn
16 Joy, K.L., Rajeshkumar, N.V., Kuttan, G. and Kuttan, R. (2000). Effect of Picrorrhiza kurroa extract on transplanted tumours and chemical carcinogenesis in mice. J. Ethnopharmacol. 71, 261-266   DOI   ScienceOn
17 Hoyos, M., Guerrero, J.M., Perez-Cano, R., Olivan, J., Fabiani, F., Garcia-Perganeda, A. and Osuna, C. (2000). Serum cholesterol and lipid peroxidation are decreased by melatonin in diet-induced hypercholesterolemic rats. J. Pineal Res. 28, 150-155   DOI   ScienceOn
18 Jagetia, G.C. and Baliga, M.S. (2004). The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: a preliminary study. J. Med. Food 7, 343-348   DOI
19 Jeena, K.J., Joy, K.L. and Kuttan, R. (1999). Effect of Emblica officinalis, Phyllanthus amarus and Picrorrhiza kurroa on N-nitrosodiethylamine induced hepatocarcinogenesis. Cancer Lett. 136, 11-16   DOI   ScienceOn
20 Kamada, T., Hata, J., Kusunoki, H., Ito, M., Tanaka, S., Kawamura, Y., Chayama, K. and Haruma, K. Eradication of Helicobacter pylori increases the incidence of hyperlipidaemia and obesity in peptic ulcer patients. Dig. Liver Dis. 37, 39- 43
21 Pedrosa, R.C., Meyre-Silva, C., Cechinel-Filho, V., Benassi, J.C., Oliveira, L.F., Zancanaro, V., Dal Magro, J. and Yunes, R.A. (2002). Hypolipidaemic activity of methanol extract of Aleurites moluccana. Phytother. Res. 16, 765- 768   DOI   ScienceOn
22 Pirat, B., Korkmaz, M.E., Eroglu, S., Tayfun, E., Yildirir, A., Ulucam, M., Ozin, B. and Muderrisoglu, H. (2004). The effects of simvastatin combined with different antioxidant vitamin regimens on serum lipid profile in patients with low HDL cholesterol levels. Anadolu Kardiyol. Derg. 4, 318-322
23 Mehrotra, R., Rawat, S., Kulshreshtha, D.K., Patnaik, G.K. and Dhawan, B.N. (1990). In vitro studies on the effect of certain natural products against hepatitis B virus. Indian J. Med. Res. 92, 133-138
24 Le, P.M., Benhaddou-Andaloussi, A., Elimadi, A., Settaf, A., Cherrah, Y. and Haddad, P.S. The petroleum ether extract of Nigella sativa exerts lipid-lowering and insulinsensitizing actions in the rat. J. Ethnopharmacol. 94, 251-259
25 Lee, H.S., Ahn, H.C. and Ku, S.K. (2006a). Hypolipemic effect of water extracts of Picrorrhiza rhizoma in PX-407 induced hyperlipemic ICR mouse model with hepatoprotective effects: A prevention study. J. Ethnopharmacol. 105, 380-386   DOI   ScienceOn
26 Lee, H.S., Yoo, C.B. and Ku, S.K. (2006b). Hypolipemic effect of water extracts of Picrorrhiza rhizoma in high fat diet treated mouse. Fitoterapia, 77, 579-584   DOI   ScienceOn
27 Milionis, H.J., Kakafika, A.I., Tsouli, S.G., Athyros, V.G., Bairaktari, E.T., Seferiadis, K.I. and Elisaf, M.S. (2004). Effects of statin treatment on uric acid homeostasis in patients with primary hyperlipidemia. Am. Heart J. 148, 635-640   DOI   ScienceOn
28 Morishita, S., Saito, T., Mishima, Y., Mizutani, A., Hirai, Y., Koyama, S. and Kawakami, M. (1986) Strains and species differences in experimental hyperlipidemia. Nippon Yakurigaku Zasshi 87, 259-264   DOI   ScienceOn
29 Tan, B.K., Tan, C.H. and Pushparaj, P.N. (2005). Anti-diabetic activity of the semi-purified fractions of Averrhoa bilimbi in high fat diet fed-streptozotocin-induced diabetic rats. Life Sci. 76, 2827-2839   DOI   ScienceOn
30 Vickers, S., Duncan, C.A., Vyas, K.P., Kari, P.H., Arison, B., Prakash, S.R., Ramjit, H.G., Pitzenberger, S.M., Stokker, G. and Duggan, D.E. (1990). In vitro and in vivo biotransformation of simvastatin, an inhibitor of HMG CoA reductase. Drug Metab. Dispos. 18, 476-483
31 Park, K.J., Kim, H.Y., Chang, B.J. and Lee, H.H. (2004). Ameliorative effects of soy 11S protein on liver damage and hyperlipidemia in alcohol-fed rats. Biol. Pharm. Bull. 27, 1636-1641   DOI   ScienceOn
32 Neaton, J.D., Kuller, L.H., Wentworth, D. and Borhani, N.O. (1984). Total and cardiovascular mortality in relation to cigarette smoking, serum cholesterol concentration and diastolic blood pressure among black and white males followed up for five years. Am. Heart J. 108, 759-769   DOI   ScienceOn
33 Olsson, B., Bohlooly-Y, M., Fitzgerald, S.M., Frick, F., Ljungberg, A., Ahren, B., Tornell, J., Bergstrom, G. and Oscarsson, J. (2005). Bovine growth hormone transgenic mice are resistant to diet-induced obesity but develop hyperphagia, dyslipidemia, and diabetes on a high-fat diet. Endocrinology 146, 920-930   DOI   ScienceOn
34 Pari, L. and Amarnath Satheesh, M.. (2004). Antidiabetic effect of Boerhavia diffusa: effect on serum and tissue lipids in experimental diabetes. J. Med. Food 7, 472-476   DOI   ScienceOn
35 Patil, U.K., Saraf, S. And Dixit, V.K. (2004). Hypolipidemic activity of seeds of Cassia tora Linn. J. Ethnopharmacol. 90, 249-252   DOI   ScienceOn
36 Regulska-Ilow, B., Biernat, J., Grajeta, H., Ilow, R. and Drzewicka, M. (2004). Influence of bioflavonoids from the radix extract of Scutellaria baicalensis on the level of serum lipids, and the development of laboratory rats fed with fresh and oxidized fats. Nahrung 48, 123-128   DOI   ScienceOn
37 Steinmetz, E.F., Buckley, C., Shames, M.L., Ennis, T.L., Vanvickle-Chavez, S.J., Mao, D., Goeddel, L.A, Hawkins, C.J. and Thompson, R.W. (2005). Treatment with simvastatin suppresses the development of experimental abdominal aortic aneurysms in normal and hypercholesterolemic mice. Ann. Surg. 41, 92-101
38 Senanayake, G.V., Maruyama, M., Shibuya, K., Sakono, M., Fukuda, N., Morishita, T., Yukizaki, C., Kawano, M. and Ohta, H. (2004). The effects of bitter melon (Momordica charantia) on serum and liver triglyceride levels in rats. J. Ethnopharmacol. 91, 257-262   DOI   ScienceOn
39 Senthil Kumar, S.H., Anandan, R., Devaki, T. and Santhosh Kumar, M. (2001). Cardioprotective effects of Picrorrhiza kurroa against isoproterenol-induced myocardial stress in rats. Fitoterapia 72, 402-405   DOI   ScienceOn
40 Shukla, R., Gupta, S., Gambhir, J.K., Prabhu, K.M. and Murthy, P.S. (2004). Antioxidant effect of aqueous extract of the bark of Ficus bengalensis in hypercholesterolaemic rabbits. J. Ethnopharmacol. 92, 47-51   DOI   ScienceOn
41 Yamashita, J. and Hayashi, S. (1990). The effect of dietary protein source on plasma cholesterol level and fecal steroid excretion in obese mice. J. Nutr. Sci. Vitaminol. (Tokyo), 36, 545-558   DOI
42 Yokozawa, T., Ishida, A., Cho, E.J. and Nakagawa, T. (2003). The effects of Coptidis Rhizoma extract on a hypercholesterolemic animal model. Phytomedicine 10, 17-22   DOI   ScienceOn
43 Yu, Y.M., Chang, W.C., Liu, C.S. and Tsai, C.M. (2004). Effect of young barley leaf extract and adlay on plasma lipids and LDL oxidation in hyperlipidemic smokers. Biol. Pharm. Bull. 27, 802-805   DOI   ScienceOn
44 Zhu, H.M. and Zhu, B.D. (2004). Experimental study on preventive effect of Radix Paeoniae Rubra to restenosis after carotid balloon injury in high fat-diet rabbits. Zhongguo Zhong Xi Yi Jie He Za Zhi, 24, 538-540
45 Zdrenghea, D., Gligor, E., Ossian, V. and Pop, D. (2004). The effect of simvastatin associated with ranitidine and alcohol upon serum lipids. Rom. J. Intern. Med. 42, 143- 148