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http://dx.doi.org/10.4062/biomolther.2007.15.4.205

Neuroprotective and Antioxidant Effects of the Butanol Fraction Prepared from Opuntia ficus-indica var. saboten  

Cho, Jung-Sook (Department of Pharmacology, College of Medicine, Dongguk University)
Han, Chang-Kyun (Life Science Research Center, SK Chemicals)
Lee, Yong-Sup (College of Pharmacy, Kyung Hee University)
Jin, Chang-Bae (Division of Life Sciences, Korea Institute of Science & Technology)
Publication Information
Biomolecules & Therapeutics / v.15, no.4, 2007 , pp. 205-211 More about this Journal
Abstract
The fruits and stems of Opuntia ficus-indica var. saboten have been reported to exhibit a variety of pharmacological actions, including antioxidant, analgesic, anti-inflammatory, and anti-ulcer effects. In the present study, we evaluated effects of the butanol fraction (SK OFB901) prepared from the 50% ethanol extract of the stems on various types of neuronal injuries induced by oxidative stress, excitotoxins, and amyloid ${\beta}\;(A_{\beta})$ in primary cultured rat cortical cells. Its antioxidant and radical scavenging activities were also evaluated by cell-free bioassays. We found that SK OFB901 strongly inhibited the oxidative neuronal damage induced by $H_2O_2$ or xanthine/xanthine oxidase. In addition, it exhibited marked inhibition of the excitotoxic neuronal damage induced by glutamate, N-methyl-D-aspartic acid, or kainate. Furthermore, the $A_{\beta(25-35)}$-induced neurotoxicity was also significantly attenuated by SK OFB901. It was found to inhibit lipid peroxidation initiated by $Fe^{2+}$ and L-ascorbic acid in rat brain homogenates and scavenge 1,1-diphenyl-2-picrylhydrazyl free radicals. These results indicate that the butanol fraction prepared from the stems of Opuntia ficus-indica var. saboten exerts potent antioxidant and neuroprotective effects through multiple mechanisms, implying its potential applications for the prevention or management of neurodegenerative disorders associated with oxidative stress, excitotoxicity, and $A{\beta}$.
Keywords
Opuntia ficus-indica; Neuroprotection; Antioxidant; Cortical culture; Excitotoxicity; Amyloid ${\beta}\; (A{\beta})$;
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