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http://dx.doi.org/10.4062/biomolther.2007.15.2.078

Effects of Proto-oncogene Protein DEK on PCAF Localization

Lee, In-Seon (Department of Life Science, College of Natural Sciences, Chung-Ang University)
Lee, Seok-Cheol (Department of Life Science, College of Natural Sciences, Chung-Ang University)
Lee, Jae-Hwi (Depatrment of Pharmaceuticals, College of Pharmacy, Chung-Ang University)
Seo, Sang-Beom (Department of Life Science, College of Natural Sciences, Chung-Ang University)

Publication Information

Biomolecules & Therapeutics / v.15, no.2, 2007 , pp. 78-82 More about this Journal

Abstract

The proto-oncogene protein DEK is a nuclear binding phosphoprotein that has been associated with various human diseases including leukemia. Histone acetylation is an important post-translational modification which plays important role in transcriptional regulation. Auto-acetylation of histone acetyltransferase PCAF results in increment of its HAT activity and facilitation of its nuclear localization. In this study, we report that DEK inhibits PCAF auto-acetylation through direct interaction. The C-terminal acidic domains of DEK are responsible for the interaction with PCAF. Using confocal microscopy, we have shown that nuclear localization of PCAF is severely inhibited by DEK. Taken together, our results suggest that DEK may be involved in various cellular signal transduction pathways accommodated by PCAF through the regulation of PCAF auto-acetylation.

Keywords

PCAF; DEK; Acetylation; Localization; Protein interaction; Nucleus;

Citations & Related Records

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