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http://dx.doi.org/10.4062/biomolther.2007.15.2.073

N,N-Dimethyl-D-ribo-phytosphingosine Modulates Cellular Functions of 1321N1 Astrocytes  

Lee, Yun-Kyung (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Kim, Hyo-Lim (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Kim, Kye-Ok (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Sacket, Santosh J. (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Han, Mi-Jin (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Jo, Ji-Yeong (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Lim, Sung-Mee (Department of Food Science & Technology, Tongmyong University)
Im, Dong-Soon (Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Research Institute of Drug Development, Pusan National University)
Publication Information
Biomolecules & Therapeutics / v.15, no.2, 2007 , pp. 73-77 More about this Journal
Abstract
N,N-Dimethyl-D-ribo-phytosphingosine (DMPH) is an N-methyl derivative of sphingosine. In the present paper, we studied effects of DMPH on intracellular Ca$^{2+}$ concentration, pH, glutamate uptake, and cell viability in human 1321N1 astrocytes. DMPH increased intracellular Ca$^{2+}$ concentration and cytosolic pH significantly in a dose-dependent manner. DMPH also inhibited glutamate uptake by 1321N1 astrocytes. Finally, treatment of cells with DMPH for 24 h reduced viability of cells largely and concentration-dependently. In summary, DMPH increased intracellular Ca$^{2+}$ concentration and pH, inhibited glutamate uptake and evoked cytotoxicity in 1321N1 astrocytes. Our observations with DMPH in the 1321N1 astrocytes would enhance understanding of DMPH actions in the brain.
Keywords
Sphingosine; Calcium; pH; Glutamate; Astrocytes;
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