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Effect of Imperatorin on Adenosine Triphosphate-stimulated Mucin Secretion from Airway Epithelial Cells  

Heo, Ho-Jin (Department of Pharmacology, College of Medicine, Chungnam National University)
Kim, Cheol-Su (Department of Pharmacology, College of Medicine, Chungnam National University)
Lee, Hyun-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
Shin, Seung-Won (Department of Pharmacy, College of Pharmacy, Duksung Women's University)
Kim, Young-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Kang, Sam-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Park, Yang-Chun (Department of Oriental Medicine, College of Oriental Medicine, Daejeon University)
Kim, Yun-Hee (Department of Oriental Medicine, College of Oriental Medicine, Daejeon University)
Seo, Un-Kyo (Department of Oriental Medicine, College of Oriental Medicine, Dongguk University)
Seok, Jeong-Ho (Department of Pharmacology, College of Medicine, Chungnam National University)
Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
Publication Information
Biomolecules & Therapeutics / v.14, no.4, 2006 , pp. 235-239 More about this Journal
Abstract
Angelicae Koreanae Radix has been used for controlling inflammatory respiratory diseases in folk medicine and their components, imperatorin, marmesinin and oxypeucedanin were reported to have diverse biological effects. In this study, we investigated whether imperatorin, marmesinin and oxypeucedanin affect adenosine triphosphate(ATP)-induced mucin secretion from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial(HTSE) cells were metabolically radiolabeled using $^3H$-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on $^3H$-mucin secretion. The results were as follows: 1) imperatorin significantly inhibited ATP-induced mucin secretion; 2) However, marmesinin and oxypeucedanin did not affect ATP-induced mucin secretion, significantly. We conclude that imperatorin might inhibit ATP-induced mucin secretion by directly acting on airway mucin-secreting cells. Therefore, imperatorin should further be investigated for the possible use as mucoregulators in the treatment of inflammatory airway diseases.
Keywords
Airway; Mucin; Imperatorin; Marmesinin; Oxypeucedanin;
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1 Kim, K.C., Wasano, K., Niles, R.M., Schuster, J.E., Stone, P.J. and Brody, J.S. (1987). Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proc. Natl. Acad. Sci. USA. 84, 9304-9308
2 Ko, K.H., Jo, M., McCracken, K. and Kim, K.C. (1997). ATP-induced mucin release from cultured airway goblet cells involves, in part, activation of protein kinase e. Am. J. Respir. Cell Mol. Biol. 16(2): 194-198   DOI
3 Ko, K.H., Lee, C.J., Shin, C.Y., Jo, M.-J. and Kim, K.C. (1999). Inhibition of mucin release from airway goblet cells by polycationic peptides. Am. J. Physiol. 277(21), L81l-L815
4 Matsuda, H., Morikawa, T., Ohgushi, T., Ishiwada, T., Nishida, N. and Yoshikawa, M. (2005). Inhibitors of nitric oxide production from the flowers of Angelica furcijuga: structures of hyuganosides IV and V. Chem. Pharm. Bull (Tokyo). 53(4): 387-392   DOI   ScienceOn
5 Piao, X.L., Park, I.H., Baek, S.H., Kim, H.Y., Park, M.K. and Park, J.H. (2004). Antioxidative activity of furanocoumarins isolated from Angelicae dahuricae. J. Ethnopharmacol. 93(23): 243-246   DOI   ScienceOn
6 Ban, H.S., Lim, S.S., Suzuki, K., Jung, S.H., Lee, S., Lee, Y.S., Shin, K.H. and Ohuchi, K. (2003). Inhibitory effects of furanocoumarins isolated from the roots of Angelica dahurica on prostaglandin E2 production. Planta Med. 69(5): 408-412   DOI   ScienceOn
7 Gapter, L., Wang, Z., Glinski, J. and Ng, K.Y. (2005). Induction of apoptosis in prostate cancer cells by pachymic acid from Poria cocos. Biochem. Biophys. Res. Commun. 332(4): 1153-1161   DOI   ScienceOn
8 Kim, K.C., McCracken, K., Lee, B.C., Shin, C.Y., Jo, M.-J, Lee, C.J. and Ko, K.H. (1997). Airway goblet cell mucin: its structure and regulation of secretion. Eur. Respir. J. 11, 2644-2649
9 Lee, C.J., Seok, J.H., Hur, G.M., Lee, J.H., Park, J.S., Seol, I.C. and Kim, Y.H. (2004a). Effects of ursolic acid, betulin and sulfur-containing compounds on mucin release from airway goblet cells. Planta Med. 70, 1119-1122   DOI   ScienceOn
10 Kim, K.C., Rearick, J.I., Nettesheim, P., and Jetten, A.M. (1985). Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J. Biol. Chem. 260, 4021-4027
11 Marquez, N., Sancho, R., Ballero, M., Bremner, P., Appendino, G., Fiebich, B.L., Heinrich, M and Munoz, E. (2004). Imperatorin inhibits T-cell proliferation by targeting the transcription factor NFAT. Planta Med. 70(11): 1016-1021   DOI   ScienceOn
12 Newhouse, M.T. and Biennenstock, J. (1983). Respiratory tract defense mechanism. In Textbook of pulmonary disease (G.L.Baum and E.Wolinsky, Ed.), Little Brown and Company, Boston
13 Shin, C.Y., Jo, M.J., Lee, W.J., Ryu, J.R., Kim, K.C. and Ko, K.H. (2001). ATP-induced mucin release from cultured airway goblet cell involves, in part, activation of phospholipase A2. Methods Find. Ex.p Clin. Pharmacol. 23(2): 73-77   DOI   ScienceOn
14 Stavri, M. and Gibbons, S. (2005). The antimycobacterial constituents of dill (Anethum graveolens). Phytother. Res. 19(1l): 938-941   DOI   ScienceOn
15 Vimal, V. and Devaki, T. (2004). Linear furanocoumarin protects rat myocardium against lipid peroxidation and membrane damage during experimental myocardial injury. Biomed. Pharmacother. 58(6-7): 393-400   DOI   ScienceOn
16 Lee, C.J., Lee, J.H., Seok, J.H., Hur, G.M., Park, J.S., Bae, S., Lim, J.H. and Park, Y.C. (2004b). Effects of betaine, coumarin and flavonoids on mucin release from cultured hamster tracheal surface epithelial cells. Phytother. Res. 18, 301-305   DOI   ScienceOn
17 Wasano, K., Kim, K.C., Niles, R.M. and Brody, J.S. (1988). Membrane differentiation markers of airway epithelial secretory cells. J. Histochem. Cytochem. 36, 167-178   DOI   ScienceOn
18 Lee, C.J., Lee, J.H., Seok, J.H., Hur, G.M., Park, Y.C., Seol, I.C. and Kim, Y.H. (2003). Effects of baicalein, berberine, curcumin and hesperidin on mucin release from airway goblet cells. Planta Med. 69, 523-526   DOI   ScienceOn