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Chunghyuldan Downregulates the Activation of Transcription Factors NF - kB and AP-1 of BV-2 Cells Induced by Lipopolysaccharide  

WEE Sung-SooK (College of Pharmacy, Kyung Hee University)
BAE Eun-Ah (College of Pharmacy, Kyung Hee University)
PARK Jin-Sun (Department of Neuroscience, Ewha Womans University Medical School)
KIM Hee-Sun (Department of Neuroscience, Ewha Womans University Medical School)
CHo Hee Jae (Kwangdong Central Research Institute, Kwangdong Pharm. Co., Ltd,)
Ryu Jong-Hoon (College of Pharmacy, Kyung Hee University)
KIM Dong-Hyun (College of Pharmacy, Kyung Hee University)
Publication Information
Biomolecules & Therapeutics / v.13, no.4, 2005 , pp. 214-219 More about this Journal
Abstract
Chunghyuldan (Qingxuedan in Chinese) (CHD) has been used for patients with atherosclerosis and brain ischemia in Korea. To evaluate antiischemic activity of CHD, its antiinflammatory effect in lipopolysaccharide-induced BV-2 cells was investigated. CHD potently inhibited nitric oxide (NO) production in LPS-induced BV-2 cells with an $IC_{50}$ value of 4.8${\mu}g/ml$. CHD did not only inhibit mRNA and protein expression levels of inducible NO synthase and cyclooxygenase-2 in LPS-induced BV-2 cells, but also repressed mRNA expression levels of proinflammatory cytokines IL-l$\beta$ and TNF-$\alpha$. CHD also downregulated the activation of NF-kB and AP-l transcription factors induced by LPS. These results suggest that CHD may improve inflammatory brain ischemia by the downregulation the activation of NF-kB and AP-l transcription factors.
Keywords
Chunghyuldan; Qingxuedan; NF-kB; AP-l; BV-2 cell;
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