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Hydrogen Peroxide Mediates Brazilin-induced Glucose Transport in Adipocytes  

Khil, Lee-Yong (College of Pharmacy, Seoul National University)
Moon, Chang-Kiu (College of Pharmacy, Seoul National University)
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Biomolecules & Therapeutics / v.12, no.4, 2004 , pp. 228-234 More about this Journal
Brazilin shows hypoglycemic effect in diabetic animals through enhancement of glucose metabolisms in insulin responsive tissues. One of the major mechanisms of brazilin to enhance glucose metabolism is stimulation of glucose transport in adipocytes. In this study, the essential molecular moiety of brazilin for the stimulation of glucose transport was investigated. We found that brazilin undergoes a structural change in physiological buffer and produces hydrogen peroxide. Methylation of hydroxyl group of brazilin or addition of catalase along with brazilin resulted in the complete inhibition of brazilin-induced glucose transport in adipocytes. Because hydrogen peroxide increases glucose transport by inhibition of phosphatases, we examined the effect of brazilin on phosphatase activity. Brazilin inhibited phosphatases in a wide range of activity, and protein phosphatase 1 and 2A were also inhibited. These results suggest that the production of hydrogen peroxide by oxidation of catechol hydroxyl group of brazilin mediates glucose transport through inhibition of phosphatases which otherwise decrease glucose transport in adipocytes.
brazilin; hydrogen peroxide; glucose transport; adipocyte; phosphatase;
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1 Martin, J.P. Jr, Dailey, M. and Sugarman, E. (1987). Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch. Biochem. Biophys. 255, 329-336   DOI   ScienceOn
2 Bialojan, C. and Takai, A. (1988). Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem. J. 256, 283-290   DOI
3 Bracke, M.E., De Pestel, G., Castronovo, V., Vyncke, B., Foidart, J.M., Vakaet, L.C. and Mareel, M.M. (1988). Flavonoids inhibit malignant tumor invasion in vitro. Prog. Clin. BioI. Res. 280, 219-233
4 Cohen, P., Alemany, S., Hemmings, B.A., Resink, T.J., Stralfors, P. and Tung, H.Y. (1988). Protein phosphatase-l and protein phosphatase-2A from rabbit skeletal muscle. Methods Enzymol. 159, 390-408   DOI
5 DeFronzo, R.A., Bonadonna, R.C. and Ferrannini, E. (1992). Pathogenesis of NIDDM. A balanced overview. Diabetes Care. 15,318-368   DOI   ScienceOn
6 Green, A. (1986). The insulin-like effect of sodium vanadate on adipocyte glucose transport is mediated at a post-insulinreceptor level. Biochem. J. 238, 663-669   DOI
7 Halliwell, B. and Gutteridge, J.M.C. (1988). Free radicals in biology and medicine. Oxford Press., UK
8 Hikino, H., Taguchi, T., Fujimura, H. and Hiramatsu, Y. (1977). The validity of oriental medicine. Planta Med. 31, 214-220   DOI   ScienceOn
9 James, D.E. and Piper, R.C. (1994). Insulin resistance, diabetes, and the insulin-regulated trafficking of GLUT-4. J. Cell BioI. 126, 1123-1126, 1994   DOI   ScienceOn
10 Jun, H., Bae, H.Y., Lee, B.R., Koh, K.S., Kim, Y.S., Lee, K.W., Kim, H. and Yoon, J. (1999). Pathogenesis of non-insulindependent (type m diabetes mellitus (NIDDM)-genetic predisposition and metabolic abnormalities. Adv. Drug Deliv. Rev. 35, 157-177   DOI   ScienceOn
11 Khil, L.Y, Han, S.S., Kim, S.G., Chang, T.S., Jeon, S.D., So, D.S. and Moon, C.K. (1999). Effects of brazilin on GLUT4 recruitment in isolated rat epididymal adipocytes. Biochem. Pharmacol. 58, 1705-1712   DOI   ScienceOn
12 Koshio, O., Akanuma, Y. and Kasuga, M. (1988). Hydrogen peroxide stimulates tyrosine phosphorylation of the insulin receptor and its tyrosine kinase activity in intact cells. Biochem. J. 250, 95-101   DOI
13 Kruszynska, Y.T. and Olefsky, J.M. (1996). Cellular and molecular mechanisms of non-insulin dependent diabetes mellitus. J. Investig. Med. 44,413-428
14 Mahadev, K., Zilbering, A., Zhu, L. and Goldstein, B.J. (2001). Insulin-stimulated hydrogen peroxide reversibly inhibits protein-tyrosine phosphatase 1b in vivo and enhances the early insulin action cascade. J. Biol. Chem. 276, 21938-21942   DOI   ScienceOn
15 Moon, C.K., Yun, Y.P., Lee, J.H., Wagner, H. and Shin, YS. (1985). Inhibition of lens-aldose reductase activity by brazilin and haematoxylin. Planta Med. 47, 66-67
16 Masaki, H., Atsumi, T. and Sakurai, H. (1995). Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem. Biophys. Res. Commun. 206, 474-479   DOI   ScienceOn
17 Moon, C.K., Lee, S.H., Chung, J.H., Won, H.S., Kim, J.Y, Khil, L.Y., et al. (1990). Effects of brazilin on glucose metabolism in isolated soleus muscles from streptozotocin induced diabetic rats. Arch. Pharm. Res. 13, 359-364   DOI
18 Moon, C.K., Lee, S.H., Lee, M.O. and Kim, S.G. (1993). Effects of Brazilin on glucose oxidation, lipogenesis and therein involved enzymes in adipose tissues from diabetic KK-mice. Life Sci. 53, 1291-1297   DOI   ScienceOn
19 Olefsky, J.M. (1975). Effect of dexamethasone on insulin binding, glucose transport, and glucose oxidation of isolated rat adipocytes. J. Clin. Invest. 56, 1499-1508   DOI
20 Sommer, D., Coleman, S., Swanson, S.A. and Stemmer, P.M. (2002). Differential susceptibilities of serine/threonine phosphatases to oxidative and nitrosative stress. Arch. Biochem. Biophys. 404,271-278   DOI   ScienceOn
21 Tanti, J.F., Grillo, S., Gremeaux, T., Coffer, P.J., Van Obberghen, E. and Le Marchand-Brustel, Y. (1997). Potential role of protein kinase B in glucose transporter 4 translocation in adipocytes. Endocrinology. 138, 2005-2010   DOI   ScienceOn
22 Tonks, N.K., Diltz, CD. and Fischer, E.H. (1988). Characterization of the major protein-tyrosine-phosphatases of human placenta. J. Biol. Chem. 263,6731-6737
23 Yang, K.M., Jeon, S.D., So, D.S. and Moon, C.K. (2000). Brazilin augments cellular immunity in multiple low dose streptozotocin (MLD-STZ) induced type I diabetic mice. Arch. Pharm. Res. 23, 626-632   DOI   ScienceOn
24 DeFronzo, R.A., Gunnarsson, R., Bjorkman, O., Olsson, M. and Wahren, J. (1985). Effects of insulin on peripheral and splanchnic glucose metabolism in noninsulin-dependent (type II) diabetes mellitus. J. Clin. Invest. 76, 149-155   DOI
25 Fidelus, R.K. (1988). The generation of oxygen radicals: a positive signal for lymphocyte activation. Cell. Immunol. 113, 175-182   DOI   ScienceOn
26 Ugi, S., Imamura, T., Maegawa, H., Egawa, K., Yoshizaki, T., Shi, K., Obata, T., Ebina, Y., Kashiwagi, A. and Olefsky, J.M. (2004). Protein phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (protein kinase B) activity in 3T3-Ll adipocytes. Mol. Cell Biol. 24, 8778-8789   DOI   ScienceOn