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Genotoxicity Study of AS6, a Triterpenoid Derivatives  

Kwon, Jung (R＆D Center of Dong Kook Pharmaceutical Co., LTD.,)
Lee, Michael (R＆D Center of Dong Kook Pharmaceutical Co., LTD.)
Cha, Kyung-Hoi (R＆D Center of Dong Kook Pharmaceutical Co., LTD.)
Kim, Jong-Choon (Department of Pharmacology and Toxicology, College of Veterinary Medicine Chonnam National University)
Han, Jung-Hee (Korea Institute of Toxicology, KRICT)
Publication Information
Biomolecules & Therapeutics / v.11, no.3, 2003 , pp. 190-195 More about this Journal
Abstract
To assess the genotoxicity of AS6, several classical toxicological tests were performed. In Ames test, AS6 did not show any transformation of revertant with or without S-9 metabolic activating system, indicating the lack of mutagenic effect of the compound. To assess clastogenic effect, in vivo micronucleus and in vitro chromosomal aberration assays were performed using male ICR mice and Chinese hamster lung (CHL) fibroblast cells, respectively. Chromosomal aberration was not induced regardless of the presence of S-9 metabolic activating system. In addition, AS6 did not cause any increase in the incidence of micronucleated polychromatic erythrocytes at any of the dose levels, suggesting little clastogenicity in vitro or in vivo. Taken together, these results demonstrate that AS-6 has no mutagenic effect in our test system.
Keywords
AS6; Ames test; In vivo micronucleus assay; In vitro chromosomal aberration assay;
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