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Effect of Proliferation Inhibition on B16/F10 Melanoma Cell by Chloroform Extract from Cornis fructus  

최원형 (원광대학교 한의학전문대학원 한약자원개발학과)
천현자 (원광대학교 자연과학기술부)
백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
Publication Information
Biomolecules & Therapeutics / v.11, no.2, 2003 , pp. 151-156 More about this Journal
Abstract
Cornis fructus have various biological effects and major chemical components have been tannins, saponins, ursolic acids, gallic acids, linoleic acids, morronisides, cornins and loganins. The main aim of the present study is measurment the effect of chloroform extract from Cornis fructus on proliferation inhibition and Cell death. Cells were cultured in the presence of chloroform extracts from Cornis fructus for 48 h. after 48h treatment of B16/F10 melanoma cells with chloroform extracts, the cells were observed a dose-dependent inhibitions of cell viability with cell death in their proliferation. the cells were estimated cell viability, cell number, total DNA fragmentation and chromatin condensation in a dose-dependent manner. It also caused cell death as measured by cell morphology, DNA fragmentation and nucleus chromatin condensation. therefore, these results suggest that chloroform extracts from C. fructus is inhibitory proliferation and is related to cell death in this cells.
Keywords
Cornis Fructus; B16/F10 melanoma cell; Cell death; Proliferation;
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1 김창민 (1997) 중야대사전, 도서출판 정담, pp. 2667-2672
2 김충섭, 박종희, 도상학 (1979) 국내에 야생하는 특용tlr물자원의 이용을 위한 연구. 한국과 학기술연구소. (1), pp 1410-1416
3 신민교, 정진섭 (1998) 도해 향약(생약)대사전, 도서출판 영림사, pp. 448-449
4 전통의학 연구소편 (1994) 본초약재 도감, 성보사, pp. 203
5 Araki, T., Yamamoto, A and Yamada, M. (1987) Accurate determination of DNA content in single cell nueleic stained with Hoechst 33258 fluorochrome at high salt concentration. Histochemistry. 87, 331-338   DOI   ScienceOn
6 Bicknell, G. R., Snowden, R. T. and Cohen, G. M. (1994) Formation of high molecular mass DNA fragments is marker of apoptosis in the human leukaemic cell line, U937. J. Cell Sci. 107, 2483
7 Buja, L. M., Eigenbrodt, M. L. and Eigenbrodt, E. H. (1993) Apoptosis and necrosis Basic types and mechanisms of cell death. Arch Pathol Lab Med. 117, 1208-1214
8 Choi, W. H., Chun, H. J., Lee, J. H., and Beak, S. H. (2003) Effects of Methanol Extract from Cornis fructus on Melanogenesis. Kor. J. Pharmacogn. 34, 70-74
9 Endo, T and Taguchi, H. (1973) Study on the constituents of Cornus Offinalis Sieb. et Zucco Yakugaku Zasshi. 93, 30-32
10 Hatano, T., Yasuhara, T. and Okuda, T. (1989) Tannins of comaceous plants. I. Cornusiins D, E and F, new dimeric and trimeric hydrolyzable tannins from Cornus officinalis. Chem. Pharm. Bull. 37. 2665-2669   DOI
11 Hatano, T., Yasuhara, T, Abc, R and Okuda, T. (1990) A Galloylated monoterpene glucoside and a dimeric hydrolsable tannin from Comus Offifinalis. Phytochemistry. 29, 2975-2978   DOI   ScienceOn
12 Hosoi, J., Abe, E., Suda, T. and Kuroki, T. (1985) Regulation of melanin synthesis of BI6 mouse melanoma cells by 1 a25-dihydroxyvitamin D3 and rctinoic acid. Cancer Res. 45, 1474-1478
13 Hearing, V. J. (1987) Mammalian monophenol monoxygenase, Methods in Euzymol. 142, 152-165
14 Hearing, V. J. (1999) Biochemical control of melanogenesis and melanosomal organization. Soc. Invest. Dermatol. 4, 24-28   DOI   ScienceOn
15 Lee, S. H., Tanaka, T., Nonaka, G. I. and Nishioka, I. (1989) Secloheptulose cligallate from Cornus officinalis. Phytochem. 28, 3469-3472   DOI   ScienceOn
16 Joo, H. K. and Jang, D. J. (1989) Effects of Shanshuyu(Comus Officinalis Sieb) tea and Market teas feeding on Hematology and Liver Function of Rat. Korean J. Dietary Culture. 4, 257-264
17 Jimbow, K., Queredo, J. W. C., Prota, G., Pitzpatric, T. B. (1999) Biology of mclanocytes, In: Freedberg, I. M., Eisen, A. Z., Wolff, K. W. et ai, cds. Dermatology in genera medicine, 5th ed. New York: McGraw-Hill Book, pp. 192-220
18 김재길 (1995) 동양전통약물 원색도감, 도서출판 영림사, pp.230
19 Lee, Y. C. Kim, Y. E., Lee, B. Y. and Kim, C. J. (1992) Chemical Compositions of Corni Fructus and Separating Properties of Its Flesh by drying. Korean J. Food Sci. Technol. 24, 447-450
20 Matinez-Esparza, M. (1998) Mechanisms and melanogenesis inhibition by tumor necrosis factor-$\alpha$ in B16/F10 mouse melanoma cells. Eur. J. Biochem. 225, 139-146
21 Miyazawa, M. and Kameoka, H. (1989) Volatile tlavor components of corni fructus. Agric. Biol. Chem. 53, 3337-3340   DOI
22 Mosmann, T. (1983) Rapid colorimetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay. J. lmmun. Methods 65, 55-63   DOI   ScienceOn
23 Gavlieli, Y., Sherman, Y. and Ben-Sasson, S. A. J. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell. Biol. 119. 493   DOI   ScienceOn
24 Nicoletti, I., Migliorati, G., Pagliacci, M. C., Grignani, F. and Riccardi, C. (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry, J. Imntunol. Methods. 139, 271   DOI   ScienceOn
25 Nagata, S. (2000) Apoptotic DNA fragmentation. Exp Cell Res. 256, 12-18   DOI   ScienceOn
26 Gaw, H. Z. and Wang, H. P. (1949) Survey of chinese drugs for presence of antibacterial substances, Science, 110, 11-1
27 Hatano, T., Ogawa, N., Kira, R., Yasuhara, T. and Okuda, T. (1989a) Tannins of comaceous plants. I. Cornusiins A, B and C, dimeric monomelic and trimeric hydrolyzable tannins from Comus officinalis, and orientation of valoneoyl group in related tannins. Chem. Pharm. Bull. 37, 2083-2090   DOI   ScienceOn
28 Wyllie, A. H. (1992) Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: an overview, Cancer Meta. Rev. 11, 95   DOI   ScienceOn
29 Yang, H. O., Choi, W. H., Jeon, B. H., Beak, S. H., and Chun, H. J. (2002) Water Extract from Cornis Fructus Regulates Melanogenesis in B 16/F1O Melanoma. Kor. J. Oriental Physiology & Pathology. 16, 818-822
30 Yamahara, J.. Mibu, H., Sawada, T., Fujirnura, H., Takino, S., Yosikawa, M. and Kitagawa, I. (1981) Biologically active principles of crude drugs. Antidiabetic principles of Corni Fructus in experimental diabetes induced by streptozotocin. Yakugaku Zasshi. 101, 86   DOI
31 Yang, T. H., Liu, S. H. and Sun, M. H. (1971) Constituents of the fruits of Cornus Officinalis. Taiwan Yao Hsueh Tsa Chih. 1, 22-26
32 Lee, E. B., Choi, B. C. and Cho, T. S. (1985) Pharmacological stuclies on ether fraction of Corni Fructus. J. Pharm. Soc. Kor. 29, 1-10
33 Kim, D. K., Kwak, J. H., Ryu, J. H., Kwon, H. C., Song, K. W, Kang, S. S., Lee, S. H., Lee, E. A., Kwon, N. S., Lee, K. R. and Zee, O. P. (1996) A Component from Cornus officinalis Enhances Hydrogen Peroxide Generation from Macrophages. Kor. J. Pharmacogn. 27, 101-104
34 Kerschmann, R. L., Woda, B. A. and Majno, G. (1995) The fusion of tumor cells with host cells; retlections on an ovarian tumor. Perspect Biol Med. 38, 467-475   DOI
35 Latt, S. A. and Steuen, G. (1976) Spectral studies on 33258 Hoechst and related bisbenzimidazole useful for fluorescent detection of deoxyribonuceic acid synthesis. J. Histocheni. Cytochem. 24, 24-33   DOI   ScienceOn
36 Wyllie, A. H., Kerri, J. F. R. and Currie, A. R. (1980) Cell death: the significance of apoptosis, lnt. Rev. Cytol. 68, 251   DOI
37 Okuda, T., Hatano, T., Ogawa, N., Kira, R. and Matsuda, M. (1984) Comusiin A, A dimeric ellagitannin forming four tautomers, and accompanying new tannins in Comus officinalis. Chem. Pharm. Bull. 32, 4662-4665   DOI
38 Qiaoling Peng, Z. W and Benjamin, E. S. Lau. (1998) Fructus corni Enhances Endothelial Cell Antioxidant Defenses. General Pharmacology. 31, 221-225   DOI   ScienceOn
39 Seo, K. I., Lee, S. W. and Yang, K. H. (1999) Antimicrobial and Antioxidaiivc activities of Corni fructus extracts. Kor. J. Postharvest Sci. Tec. 6, 99-103