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Hematopoietic Efficacy of Recombinant Human Erythropoietin (DWP413)  

최현주 (주)대웅제약 생명과학연구소)
김점용 (주)대웅제약 생명과학연구소)
임승욱 (주)대웅제약 생명과학연구소)
연제덕 (주)대웅제약 생명과학연구소)
고여욱 (주)대웅제약 생명과학연구소)
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Biomolecules & Therapeutics / v.11, no.2, 2003 , pp. 126-132 More about this Journal
Abstract
Efficacy and in vivo bioassay of recombinant human erythropoietin (rh-EPO, DWP413) was investigated. Efficacy studies on erythropoiesis were conducted in normal, cisplatin-induced anemic rats and acute hemorrhage - induced anemic rats. Animals were treated intravenously with DWP413 for 5 days, the changes in the number of red blood cells (RBC), hematocrit value (Hct), hemoglobin concentration (Hb) and reticulocyte were examined. In normal rats, at the doses of 50, 250, and 1250 IU/kg/day, in cisplatin-induced anemic rats, at the doses of 50, 100 and 200 IU/kg/day, RBC, Hb, Hct and reticulocyte were increased dose-depen-dently. And in acute hemorrhage-induced anemic rats, DWP413 (150, 450 and 1350 IU/kg/day) significantly increased RBC, Hb, Hct and reticulocytes. In histopathological findings of kidney, cisplatin alone treated rats expressed severe glomerulus and tubular damage. But in the DWP413 treated rats after cisplatin treatment, these were not remarkable compared to cisplatin alone treated rats. In vivo bioassay, DWP413 had 102.43% of bioactivity compared to erythropoietin BRP(Biological Reference Product, European Directorate for the Quality of Medicines). These results suggest that DWP413 might be useful for the therapy of anemia induce by renal failure and acute blood loss.
Keywords
DWP413; efficacy; bioassay; erythropoiesis; acute hemorrhage induced rat; cisplatin; normocythemic mice;
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1 Anagnostou, A. (1985). Erythropoietin: a hematopoietic hormone produced by the kidney. Semin. Nephrol. S, 104-114
2 Edward A., Levine, M.D., Arthur L., Rosen, Ph. D., Laksman R., Sehgal, Ph. D., Steven A., Gould, M.D., Joan C., Eglie, Ph. D., Ransa L., Sehgal, B.S.M.T., and Gerald S.Moss. M.D. (1989). Treatment of acute postoperative anemia with recombinant human erythropoietin. J.Trauma, 29, 1134-1139   DOI
3 Masunaga, H., Goto, M. and Ueda, M. (1986). Effects of purified erythropoietin in partially nephrectomized rats. Acta Hematol. Jpn. 49, 807-815
4 Masunaga, H., Takahira, Rand Sawai, T. (1991). Effects of recombinant human erythropoietin on renal anemic rats induced by drugs. Folia. Pharmacol. Jpn. 98, 151-160   DOI   ScienceOn
5 McGonigle R. J., Brookins J, Pegram B. L. Fisher J.W (1987) : Enhanced erythropoietin production by calcium entry blockers in rats exposed to hypoxia. J. Pharmacol. Exp. Then 241, 428-432
6 Fuchs, A. and Eder, H. (1992). Dose dependenl effects of recombinant human erythropoietin on tile reticulocyte population of rabbits. Arzeim.-Forsch./Drug. Res. 42. 10, 1266-1268
7 Vaziri N. D., ZhoLl X. J., and Liao S. Y. (1994). Erythropoietin enhances recorvery from cisplatin-induccd acute renal failure. Am. J. Physiol. 266(Renal Fluid Electrolyte Physiol 35): F360-F366   DOI
8 Wood, P. A. and Hrushesky, W. J. M. (1995). Cisplatinassociated anemia; an erythropoietin deficiency syndrome. J. Clin. Invest. Inc. 95, 1650-1659   DOI   ScienceOn
9 Emest B., Marshall A.L., Barry S.C., Thoman J.K. (ed.)(1995). Williams Hematology pp.704-708. McGraw-Hill, New York
10 Jacobs, K, Shoemaker, C., Rudersdorf, R., Neill, S. D., Kaufman, R. J, Mufson, A, Seelu'a, J., jones, S. S., Hewick, R, Fritsch, E. E, Kawakita, M., Shimizu, T and Miyake, T. (1985). Isolation and characterization of genomic and cDNA clones of human erythropoietin. Nature 313, 806-810.   DOI   ScienceOn
11 Kuzur M. E. and Greco F. A. (1980). Cisplatin induced anemia. New Engl. J. Med. 303, 110-112
12 Joseph, W E. and John, W A (1989). Guideline for recombinant human erythropoietin therapy. Am. J. Kidney Dis. 2, 2-8
13 Kim D., H., Ahn B. O., Park J. H., Lee S. H., and Kim W. B. (1998). Efficacy of recombinant human erythropoietin (rhuEPO). J. Appl. Pharmacol. 6,312-316
14 Kim S. J., Ha B. J, Lee D. E., Oh M. S., Kim D. H., Park K. H. and Kim H. S. (1994). Efficacy of recombinant erythropoietin from CHO cells. J. Appl. Phamiacol. 2, 343-346
15 Landschulz K. T., Boyer S. H., Noyes A. n, Rogers O. C, and Frelin L. P. (1992). Onset of erythropoietin response in murine erythroid colony forming units: assignment to early S-phasc in a specific cell generation. Blood, 79, 2749-2758
16 Levine E. A, Rosen A. L., Sehgal L. R., Gould S. A, Egrie j. C, Sehgal H. L and Moss G. S. (1989). Treatment of acute postoperative anemia with recombinant human erythropoietin, J. Trauma 29, 1134-1139   DOI
17 Nam C. J., Kim H., Huh J. W, Kang J. K. (1989). Efficacy of recombinant human erythropoietin. Korean J. of the Lab. Anim. Sci. 14, 241-249
18 Nathan D. G., Syikowski A. (1983). Erythropoietin nd the regulation of erythropoiesis. N Engl. J. Med. 306, 520-522
19 Palmeri S., Russo A., Gebbia V, Borsellino N., Gebbia N., Rustum Y., Rausa L. (1990). A phase I-II study on the toxicity and therapeutic efficacy of 5-fluorouracil in cornbinatjion with leucovorin and cisplatin in patients with advanced colorectal carcinoma. J. Chemotherapy suppl. I, 28-32
20 Ridley, D. M., Dawkins, F. and perlin, E. (1994). Erythropoietin : A review. J. Natl. Med. Assoc. 86, 129-135
21 Rothmann, S. A., Paul P., Weick, J. K., Mclntyre, W. R. and Fantelli. F. (1985). Effects of cis-diamminedichloroplatinum on erythropoietin production and hematopoietic progenitor cells. Int. J. Cell Cloning 3, 415-423   DOI   ScienceOn
22 Roberta L. Scipioni, Richard W. Diters, William R. Myers, and Susan M. Hart. Clinical and clinicopathological assessment of serial phlebotomy in the Sprague Dawley rat. Laboratory Ani. Sci. 1997. 47, 293-299
23 Rossof, A. H., Slayton, R. E. and Pcrlia, C. P. (1972). Preliminary clinical experience with cisdiamminedichloroplatinum (II). Cancer 30, 1451-1450   DOI   ScienceOn
24 Rathmann S. A. and Wcik J. K. (1981). Cisplatin-induced anemia. New. Eng. J. Med. 303, 110-112.
25 Tsuda, E., Goto, M., murakarni, A, Akai, K., Ueda, M., Kawanishi, G., Takakashi, N., Sasaki, R., Chiba, H., ishihara, H., Mori, M., Tejima, S., Endo, S. and Arata, Y. (1988). Comparative structural study of N-linked oligosaccharides of urinary and recombinant eryihropoietins. Biochemistry 27, 5646-5654   DOI   ScienceOn