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Inhibition of Glutamate-Induced Change in Mitochondrial Membrane Permeability in PC12 cells by 1-Methylated β-carbolines  

Han, Eun-Sook (Department of Pharmacology, College of Medicine, Chung-Ang University)
Lee, Chung-Soo (Department of Pharmacology, College of Medicine, Chung-Ang University)
Publication Information
Biomolecules & Therapeutics / v.11, no.2, 2003 , pp. 112-118 More about this Journal
Abstract
1-Methylated $\beta$-carbolines (harmaline and harmalol) and antioxidants (N-acetylcysteine and ascorbate) reduced the loss of cell viability in differentiated PC 12 cells treated with 5 mM glutamate. $\beta$-Carbolines prevented the glutamate-induced decrease in mitochondrial membrane potential, cytochrome c release and caspase-3 activation in PC 12 cells. $\beta$-Carbolines reduced the formation of reactive oxygen species and depletion of glutathione due to glutamate in PC12 cells. $\beta$-Carbolines revealed a scavenging action on hydrogen peroxide and reduced the iron and EDTA-mediated degradation of 2-deoxy-D-ribose. The results suggest that I-methylated $\beta$-carbolines attenuate the cytotoxic effect of glutamate on PC12 cells by reducing the alteration of mitochondrial membrane permeability that seems to be mediated by oxidative stress.
Keywords
$\beta$-carbolines; glutamate; mitochondrial membrane permeability; PC12 cells;
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