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Cytotoxic Activities and Antioxidative Activities Against Liver Cancer Cell of Albizzia root  

강병수 (동국대학교 한의과 대학 본초학교실)
이갑득 (동국대학교 자연과학대학 화학과)
Publication Information
Biomolecules & Therapeutics / v.10, no.4, 2002 , pp. 287-292 More about this Journal
Abstract
To find new inhibitory effects from oriental drugs, Albizziae root was extracted in methanol and the extracted was stepwisely fractionated by hexane, chloroform, ethylacetate, butanol and water. In cytotoxic effect of Albizziae root fractions against cancer cell lines including human hepatoma cells(HepG2) were investigated. Expecially the butanol fraction exhibited a inhibition effects on the growth of human hepatoma cells(HepG2). It inhibited of HepG2 cells with the value of IC50. The activities of qutathione after B(a)P treatment were markedly decreased than control, but those levels were increased by the treatment of Albizziae root methanol fraction. The activity of glutathione-S-transferase after B(a)P treatment were markedly decreased than control, but those levels were increased by the treatment of Albizziae root methanol traction. Induction of phase II enzymes is a major mechanism of chemoprevention. The induction levels of quinone reductase(QR) activity in cultured murine hepatoma(Hepa IcIc7)cell by methanol extract of Albizziae root were measured. Among the tested tractions, the extracts of butanol were found to induce QR activities over 2.8 fold than control. These results suggest that Albizziae root has chemopreventive Potential by inducing QR activities and GST levels and increasing GSH
Keywords
Albizziae root; organ toxicity; chemopreventive potential; gutathione; quinone reductase; glutathione s-transferase;
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