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Inhibitory Effects of Simazine on Various Functions of Peritoneal Macrophages  

김경란 (성균관대학교 약학대학)
손은화 (성균관대학교 약학대학)
이동권 (성균관대학교 약학대학)
표석능 (성균관대학교 약학대학)
Publication Information
Biomolecules & Therapeutics / v.10, no.4, 2002 , pp. 224-229 More about this Journal
Abstract
Triazine herbicide has been reported to directly suppress the immune response. In the present study, we examined various functions of murine peritoneal macrophages that were isolated and stimulated with LPS after simazine (300 and 600 mg/kg body weight), a triazine herbicide, was administered every day for 4 weeks. Simazine decreased the capacity of phagocytosis, compared to those of carboxymethylcellulose (CMC)-treated control group. In addition, the production of NO and TNF-$\alpha$ was decrcased in macrophages of simazinetreated mice. However, the production of hydrogen peroxide ($H_{2}O_{2}$) was not altered. In vitro tumoricidal activity of in vivo simazine-treated macrophages was reduced against target cell. B 16 melanoma. Taken together, these results suggested that simazine might have the immunosuppressive effect on macrophages after in vivo exposure, which was related to the reduction of tumoricidal activity.
Keywords
Simazine; macrophage; $H_{2}O_{2}$; TNF-$\alpha$; NO; tumoricidal activity;
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