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http://dx.doi.org/10.14402/jkamprs.2013.35.3.143

Induction of Selective Cell Death of Oral Squamous Carcinoma Cells by Integrin α2 Antibody and EGFR Antibody  

Choi, Yeon-Sik (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Yoon, Sik (Department of Prothodontics, School of Dentistry, Pusan National University)
Hwang, Dae-Seok (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
Kim, Cheol-Hun (Department of Oral and Maxillofacial Surgery, College of Medicine, Dong-A University)
Jeon, Young-Chan (Department of Prothodontics, School of Dentistry, Pusan National University)
Byun, June-Ho (Department of Oral and Maxillofacial Surgery, School of Medicine, Gyeongsang National University)
Shin, Sang-Hun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.35, no.3, 2013 , pp. 143-154 More about this Journal
Abstract
Purpose: This study was to find efficacy of integrin alpha2 (${\alpha}_2$) and epidermal growth factor receptor (EGFR) as tumor marker of oral squamous cell carcinoma (SCC) and clarify the selective cell death effect of anti-integrin ${\alpha}_2$ and anti-EGFR on SCC cells, additionally testify conjugated gold nanoparticles (GNP) with air plasma for selective cell death of oral SCC. Methods: Expression of integrin ${\alpha}_2$, EGFR on human SCC cells (SCC25) were examined by western blot. SCC25 cells were treated with anti-integrin ${\alpha}_2$, anti-EGFR and analysed by Hemacolor staining, immunoflorescence staining, FACS flow cytometry. Conjugated GNP with integrin ${\alpha}_2$, EGFR antibody were treated by air plasma on SCC cells. Results: Integrin ${\alpha}_2$ and EGFR were over-expressed on SCC25 cells than normal lung WI-38 cells. The cell viability rate of SCC25 cells treated with anti-integrin ${\alpha}_2$, anti-EGFR was lower than WI-38 cells. The concentration changes of nucleus, releasing cytochrome c and apoptosis inducing factor (AIF) from mitochondria to cytosol were observed. The changes of proteins related with apoptosis were observed. Increase of bax, bcl-xL, activation of caspase-3, -7, -9, and fragmentation of PARP, DFF45 and decrease of lamin A/C in SCC25 cells were observed. In FACS, increase of sub-$G_1$ and S phase was observed. Cell cycle related proteins, Such as cyclin D1, cyclin dependent kinase (CDK) 4, cyclin A, cyclin E, CDK 2, p27 were decreased. After SCC25 cells treated with conjugatged GNP-Integrin ${\alpha}_2$, GNP-EGFR, additionally air plasma, the cell death rate was significantly increased. Conclusion: Integrin ${\alpha}_2$, EGFR were over-expressed in oral SCC cells. Anti-integrin ${\alpha}_2$, anti-EGFR in SCC25 cells induced apoptosis selectively. When GNP-anti integrin ${\alpha}_2$, GNP-anti EGFR were treated with air plasma on SCC25 cells, cancer cells were died more selectively. GNP-anti integrin ${\alpha}_2$, GNP-anti EGFR with air plasma could be treatment choice of oral SCC.
Keywords
OSCC; Integrin alpha2; EGFR; Gold nanoparticles; Cell death;
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1 Neve RM, Lane HA, Hynes NE. The role of overexpressed HER2 in transformation. Ann Oncol 2001;12 Suppl 1:S9-13.   DOI
2 Ullrich A, Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell 1990;61:203-12.   DOI   ScienceOn
3 Young NR, Liu J, Pierce C, et al. Molecular phenotype predicts sensitivity of squamous cell carcinoma of the head and neck to epidermal growth factor receptor inhibition. Mol Oncol 2012. pii: S1574-7891(12)00121-4.
4 Markovic A, Chung CH. Current role of EGF receptor monoclonal antibodies and tyrosine kinase inhibitors in the management of head and neck squamous cell carcinoma. Expert Rev Anticancer Ther 2012;12:1149-59.   DOI   ScienceOn
5 Kim KW, Kim MJ. Expression of the epidermal growth factor receptor and cell cycle analysis in the head and neck squamous cell carcinomas. J Korean Assoc Oral Maxillofac Surg 2000;26:154-63.
6 Moon BC, Han SJ, Jeong D, Kim KW. Epidermal growth factor receptor overexpression and K-ras mutation detection in the oral squamous cell carcinoma. J Korean Assoc Oral Maxillofac Surg 2011;37:396-402.   DOI   ScienceOn
7 Fridman G, Fridman G, Gutsol A, Shekhter AB, Vasilets VN, Fridman A. Applied plasma medicine. Plasma Process Polym 2008;5:503-33.   DOI   ScienceOn
8 Fridman G, Shereshevsky A, Jost MM, et al. Floating elctrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines. Plasma Chem Plasma Process 2007;27:163-76.   DOI   ScienceOn
9 Kim CH, Kwon S, Bahn JH, et al. Effects of atmospheric nonthermal plasma on invasion of colorectal cancer cells. Appl Phys Lett 2010;96:243701.   DOI   ScienceOn
10 Kim D, Gweon B, Kim DB, Choe W, Shin JH. A feasibility study for the cancer therapy using cold plasma. ICBME Proc 2008;23:355-7.
11 Kim GC, Lee HJ, Shon H. The effects of a micro plasma on melanoma (G361) cancer cells. J Korean Phys Soc 2009;54:628-32.   DOI   ScienceOn
12 Connor EE, Mwamuka J, Gole A, Murphy CJ, Wyatt MD. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small 2005;1:325-7.   DOI   ScienceOn
13 Thomas M, Klibanov AM. Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells. Proc Natl Acad Sci U S A 2003;100:9138-43.   DOI   ScienceOn
14 Levy JM, Thorburn A. Targeting autophagy during cancer therapy to improve clinical outcomes. Pharmacol Ther 2011;131:130-41.   DOI   ScienceOn
15 Chen CS, Alonso JL, Ostuni E, Whitesides GM, Ingber DE. Cell shape provides global control of focal adhesion assembly. Biochem Biophys Res Commun 2003;307:355-61.   DOI   ScienceOn
16 Riveline D, Zamir E, Balaban NQ, et al. Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism. J Cell Biol 2001;153:1175-86.   DOI   ScienceOn
17 Zamir E, Geiger B. Molecular complexity and dynamics of cell-matrix adhesions. J Cell Sci 2001;114:3583-90.
18 Ennis BW, Lippman ME, Dickson RB. The EGF receptor system as a target for antitumor therapy. Cancer Invest 1991;9:553-62.   DOI   ScienceOn
19 Mendelsohn J, Baselga J. Epidermal growth factor receptor targeting in cancer. Semin Oncol 2006;33:369-85.   DOI   ScienceOn
20 Ghavami S, Hashemi M, Ande SR, et al. Apoptosis and cancer: mutations within caspase genes. J Med Genet 2009;46:497-510.   DOI   ScienceOn
21 Dejean LM, Martinez-Caballero S, Manon S, Kinnally KW. Regulation of the mitochondrial apoptosis-induced channel, MAC, by BCL-2 family proteins. Biochim Biophys Acta 2006;1762:191-201.   DOI   ScienceOn
22 Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6.   DOI   ScienceOn
23 Dejean LM, Martinez-Caballero S, Kinnally KW. Is MAC the knife that cuts cytochrome c from mitochondria during apoptosis? Cell Death Differ 2006;13:1387-95.   DOI   ScienceOn
24 Alnemri ES, Livingston DJ, Nicholson DW, et al. Human ICE/CED-3 protease nomenclature. Cell 1996;87:171.   DOI   ScienceOn
25 Murphy KM, Ranganathan V, Farnsworth ML, Kavallaris M, Lock RB. Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells. Cell Death Differ 2000;7:102-11.   DOI   ScienceOn
26 Yu SW, Andrabi SA, Wang H, et al. Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death. Proc Natl Acad Sci U S A 2006;103:18314-9.   DOI   ScienceOn
27 Widłak P. The DFF40/CAD endonuclease and its role in apoptosis. Acta Biochim Pol 2000;47:1037-44.
28 Karam JA. Apoptosis in carcinogenesis and chemotherapy. Netherlands: Springer; 2009.
29 Morgan DO. Principles of CDK regulation. Nature 1995;374:131-4.   DOI   ScienceOn
30 Sherr CJ. G1 phase progression: cycling on cue. Cell 1994;79:551-5.   DOI   ScienceOn
31 Pines J. Cyclins: wheels within wheels. Cell Growth Differ 1991;2:305-10.
32 Si X, Liu Z. Expression and significance of cell cycle-related proteins Cyclin DI, CDK4, p27, E2F-I and Ets-1 in chondrosarcoma of the jaws. Oral Oncol 2001;37:431-6.   DOI   ScienceOn
33 Kim GC, Kim GJ, Park SR, et al. Air plasma coupled with antibody-conjugated nanoparticles: a new weapon against cancer. J Phys D Appl Phys 2009;42:032005.   DOI   ScienceOn
34 Herbst RS. Review of epidermal growth factor receptor biology. Int J Radiat Oncol Biol Phys 2004;59(2 Suppl):21-6.   DOI   ScienceOn
35 Choi BB, Choi YS, Lee HJ, Lee JK, Kim UK, Kim GC. Nonthermal plasma-mediated cacer cell death: targeted cancer treatment. J Therm Sci Technol 2012;7:399-404.   DOI   ScienceOn
36 Hughes AL. Evolution of the integrin alpha and beta protein families. J Mol Evol 2001;52:63-72.   DOI   ScienceOn
37 Guo W, Giancotti FG. Integrin signalling during tumour progression. Nat Rev Mol Cell Biol 2004;5:816-26.   DOI   ScienceOn
38 Guo L, Zhang F, Cai Y, Liu T. Expression profiling of integrins in lung cancer cells. Pathol Res Pract 2009;205:847-53.   DOI   ScienceOn
39 Bargmann CI, Hung MC, Weinberg RA. The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature 1986;319:226-30.   DOI   ScienceOn
40 Ishitoya J, Toriyama M, Oguchi N, et al. Gene amplification and overexpression of EGF receptor in squamous cell carcinomas of the head and neck. Br J Cancer 1989;59:559-62.   DOI   ScienceOn