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In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold  

Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Gyeongsang National University, Biomedical Center (BK21))
Lee, Jin-Ho (Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University)
Oh, Se-Heang (Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University)
Kim, Sang-June (Department of Advanced Materials, College of Life Science and Nano Technology, Hannam University)
Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital)
Jeon, Ryoung-Hoon (Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University)
Maeng, Geun-Ho (Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University)
Rho, Gyu-Jin (Department of Obstetrics/Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University)
Kim, Jong-Ryoul (Maxillofacial Center, On Hospital)
Byun, June-Ho (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Gyeongsang National University, Biomedical Center (BK21))
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.34, no.6, 2012 , pp. 384-390 More about this Journal
Abstract
Purpose: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold. Methods: Two one-year-old miniature pigs were used in this study. $2{\times}10^6$ periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco's modified Eagle's medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed. Results: New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery. Conclusion: These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.
Keywords
Periosteal-derived cells; Polydioxanone scaffold; In vivo osteogenesis;
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1 Park BW, Byun JH, Lee SG, et al. Evaluation of osteogenic activity and mineralization of cultured human periosteal- derived cells. J Korean Assoc Maxillofac Plast Reconstr Surg 2006;28:511-9.
2 Chew SA, Kretlow JD, Spicer PP, et al. Delivery of plasmid DNA encoding bone morphogenetic protein-2 with a biodegradable branched polycationic polymer in a critical-size rat cranial defect model. Tissue Eng Part A 2011;17:751-63.   DOI   ScienceOn
3 Li J, Li Y, Ma S, Gao Y, Zuo Y, Hu J. Enhancement of bone formation by BMP-7 transduced MSCs on biomimetic nano-hydroxyapatite/polyamide composite scaffolds in repair of mandibular defects. J Biomed Mater Res A 2010;95:973-81.
4 Zhao L, Tang M, Weir MD, Detamore MS, Xu HH. Osteogenic media and rhBMP-2-induced differentiation of umbilical cord mesenchymal stem cells encapsulated in alginate microbeads and integrated in an injectable calcium phosphate-chitosan fibrous scaffold. Tissue Eng Part A 2011;17:969-79.   DOI   ScienceOn
5 Hild N, Schneider OD, Mohn D, et al. Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells. Nanoscale 2011;3:401-9.   DOI   ScienceOn
6 Oh SH, Kang SG, Kim ES, Cho SH, Lee JH. Fabrication and characterization of hydrophilic poly(lactic-co-glycolic acid)/poly( vinyl alcohol) blend cell scaffolds by melt-molding particulate- leaching method. Biomaterials 2003;24:4011-21.   DOI   ScienceOn
7 Jeong WK, Oh SH, Lee JH, Im GI. Repair of osteochondral defects with a construct of mesenchymal stem cells and a polydioxanone/poly(vinyl alcohol) scaffold. Biotechnol Appl Biochem 2008;49:155-64.   DOI   ScienceOn
8 Itala AI, Ylanen HO, Ekholm C, Karlsson KH, Aro HT. Pore diameter of more than 100 microm is not requisite for bone ingrowth in rabbits. J Biomed Mater Res 2001;58:679-83.   DOI   ScienceOn
9 Gotterbarm T, Breusch SJ, Schneider U, Jung M. The minipig model for experimental chondral and osteochondral defect repair in tissue engineering: retrospective analysis of 180 defects. Lab Anim 2008;42:71-82.   DOI   ScienceOn
10 Martinez-Gonzalez JM, Cano-Sanchez J, Campo-Trapero J, Gonzalo-Lafuente JC, Diaz-Regaonn J, Vazquez-Pineiro MT. Evaluation of minipigs as an animal model for alveolar distraction. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;99:11-6.   DOI   ScienceOn
11 Nair LS, Laurencin CT. Polymers as biomaterials for tissue engineering and controlled drug delivery. Adv Biochem Eng Biotechnol 2006;102:47-90.
12 Borland S, Rao M, Fraser J. Interposition arthoplasty of the first carpal-metacarpal joint using suture anchor and polydioxanone ribbon. Tech Hand Up Extrem Surg 2009;13: 120-3.   DOI   ScienceOn
13 Rocha LB, Goissis G, Rossi MA. Biocompatibility of anionic collagen matrix as scaffold for bone healing. Biomaterials 2002;23:449-56.   DOI   ScienceOn
14 Wiesmann HP, Nazer N, Klatt C, Szuwart T, Meyer U. Bone tissue engineering by primary osteoblast-like cells in a monolayer system and 3-dimensional collagen gel. J Oral Maxillofac Surg 2003;61:1455-62.   DOI   ScienceOn
15 Koh CJ, Atala A. Tissue engineering, stem cells, and cloning: opportunities for regenerative medicine. J Am Soc Nephrol 2004;15:1113-25.   DOI   ScienceOn
16 Rumpler M, Woesz A, Varga F, Manjubala I, Klaushofer K, Fratzl P. Three-dimensional growth behavior of osteoblasts on biomimetic hydroxylapatite scaffolds. J Biomed Mater Res A 2007;81:40-50.
17 Lee JH, Oh SH, Park BW, et al. Osteogenic activity of cultured human periosteal-derived cells in a three dimensional polydioxanone/ pluronic f127 scaffold. J Korean Assoc Maxillofac Plast Reconstr Surg 2009;31:478-84.
18 Tang H, Xu Z, Qin X, et al. Chest wall reconstruction in a canine model using polydioxanone mesh, demineralized bone matrix and bone marrow stromal cells. Biomaterials 2009;30:3224-33.   DOI   ScienceOn
19 Stivaros SM, Williams LR, Senger C, Wilbraham L, Laasch HU. Woven polydioxanone biodegradable stents: a new treatment option for benign and malignant oesophageal strictures. Eur Radiol 2010;20:1069-72.   DOI   ScienceOn
20 Zeltinger J, Sherwood JK, Graham DA, Müeller R, Griffith LG. Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng 2001;7:557-72.   DOI   ScienceOn
21 Kneser U, Voogd A, Ohnolz J, et al. Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute. Cells Tissues Organs 2005;179:158-69.   DOI   ScienceOn
22 Arpornmaeklong P, Suwatwirote N, Pripatnanont P, Oungbho K. Growth and differentiation of mouse osteoblasts on chitosan- collagen sponges. Int J Oral Maxillofac Surg 2007;36:328-37.   DOI   ScienceOn