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The Effect of Bone Marrow-Derived Osteoblasts on Mandibular Deffect in Rabbit  

Park, Young-Ju (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Nam, Jeong-Hun (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Kim, Bo-Gyun (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Jeon, Min-Su (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Chung, Jae-An (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Lee, Jung-Won (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Ahn, Jang-Hoon (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Gang, Tae-In (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Park, Mi-Hee (Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Hallym University)
Lim, Sung-Chul (Department of Pathology, College of Medicine, Chosun University)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.32, no.4, 2010 , pp. 306-312 More about this Journal
Abstract
Purpose: The purpose of this experiment was to evaluate the clinical effect of cultured autoglogous osteoblasts as a way to treat the defect of mandible in rabbits. Materials and Methods: Twelve rabbits were used to determine the rate of osteogenesis. The osteoblasts were obtained from the iliac crest of rabbits using aspiration. They were then cultured in Dulbecco's Modified Eagles's Medium (DMEM) with beta-glycerophosophatate, L-ascorbicacid, and dexamethasone to proliferate and differentiate osteoprogenitor cells. The expression of osteogenic markers were detected by reverse transcription-polymerase chain reaction (RT-PCR) and silver nitrate staining techniques. Five, 10-mm holes were placed in each rabbit mandible to simulate defective regions with the use of a low speed trephine bur. In the experimental group, the previously cited defects were grafted with both activated osteoblastic and autogenous bone. The control group, however, was only grafted with autogenous bone. Both groups were then analyzed at 2, 4, and 8-week intervals using bone histomorphometric analysis. Results: According to histomorphologic analysis, the rates of new bone formation at the 2, 4, and 8-week intervals were 36%, 51%, and 23% for the control group, respectively; 52%, 39%, and 28%, for the experimental group, respectively. The experimental group showed higher rates of new bone formation compared to the control group at both the 2-week and 8-week interval. Conclusion: Bone marrow-derived osteoblasts seems to be a promising bone graft material.
Keywords
Osteoblasts; Autogenous bone; Histomorphometric analysis;
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