Browse > Article

Electrospun Silk Nano-Fiber Combined with Nano-Hydoxyapatite Graft for the Rabbit Calvarial Model  

Kye, Jun-Young (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
Kim, Min-Keun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
Kwon, Kwang-Jun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
Park, Young-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
Kim, Jwa-Young (Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University)
Lee, Min-Jung (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Park, Young-Hwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.32, no.4, 2010 , pp. 293-298 More about this Journal
Abstract
Purpose: The objective of the present study was to determine the capability of electrospun silk fibroin as a biomaterial template for bone formation when mixed with nano-hydoxyapatite in vivo. Materials and Methods: Ten New Zealand white rabbits were used for this study and bilateral round shaped defects were formed in the parietal bone (diameter: 8.0 mm). The electrospun silk fibroin was coated by nano-hydroxyapatite and grafted into the right parietal bone (experimental group). The left side (control group) did not receive a graft. The animals were sacrificed at 6 weeks and 12 weeks, humanly. The microcomputerized tomogram (${\mu}CT$) was taken for each specimen. Subsequently, they were undergone decalcification and stained for the histological analysis. Results: The average value of all measured variables was higher in the experimental group than in the control at 6 weeks after the operation. BMC in the experimental group at 6 weeks after operation was $48.94{\pm}19.25$ and that in the control was $26.17{\pm}16.40$ (P = 0.027). BMD in the experimental group at 6 weeks after operation was $324.59{\pm}165.24$ and that in the control was $173.03{\pm}120.30$ (P = 0.044). TMC in the experimental group at 6 weeks after operation was $19.50{\pm}6.00$ and that in the control was $10.52{\pm}6.20$ (P = 0.011). TMD in the experimental group at 6 weeks after operation was $508.88{\pm}297.57$ and that in the control was $273.54{\pm}175.91$ (P = 0.06). Gross image of both groups showed higher calcification area at 12 weeks than them in 6 weeks. The average value of ${\mu}CT$ analysis was higher at 12 weeks than that in 6 weeks in both groups. BMC in the experimental group at 12 weeks after operation was $51.21{\pm}8.81$ and that in the control was $33.47{\pm}11.13$ (P = 0.010). BMD in the experimental group at 12 weeks after operation was $323.39{\pm}21.54$ and that in the control was $197.75{\pm}76.23$ (P = 0.012). TMC in the experimental group at 12 weeks after operation was $21.44{\pm}5.30$ and that in the control was $13.31{\pm}4.17$ (P = 0.008). TMD in the experimental group at 12 weeks after operation was $524.47{\pm}19.37$ and that in the control was $299.60{\pm}136.20$ (P = 0.016). Conclusion: The rabbit calvarial defect could be successfully repaired by electrospun silk nano-fiber combined with nano-hydroxyapatite.
Keywords
Silk fibroin; Hydroxyapatite; Calvaria;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bergmans L, Van Cleynenbreugel J, Wevers M et al : A methodology for quantitative evaluation of root canal instrumentation using microcomputed tomography. Int Endod J 34 : 390, 2001.   DOI   ScienceOn
2 Horan RL, Antle K, Collette AL et al : In vitro degradation of silk fibroin. Biomaterials 26 : 3385, 2005.   DOI   ScienceOn
3 Patel V, Issever AS, Burghardt A et al : Micro-CT evaluation of normal and osteoarthritic bone structure in human knee specimens. J Orthop Res 21 : 6, 2003.   DOI   ScienceOn
4 Costantino PD, Friedman CD : Synthetic bone graft substitutes. Otolaryngol Clin North Am 27 : 1037, 1994.
5 Cao Y, Wang B : Biodegradation of silk biomaterials. Int J Mol Sci 10 : 1514, 2009.   DOI   ScienceOn
6 Van Oosterwyck H, Vander SJ, Van der PG et al : The use of microfocus computerized tomography(micro-CT) as a new technique to characterized bone tissue around oral implants. J Oral Implantol 26 : 5, 2000.   DOI   ScienceOn
7 Sennerby L, Wennerberg A, Pasop F : A new microtomographic technique for non-invasive evaluation of the bone structure around implants. Clin Oral Implants Res 12 : 91, 2001.   DOI   ScienceOn
8 Wagner JR : A 3 1/2-year clinical evaluation of resorbable hydroxylapatite osteogen (HA Resorb) used for sinus lift augmentations in conjunction with the insertion of endosseous implants. J Oral Implantol 17 : 152, 1991.
9 Wagner JR : A clinical and histological case study using resorbable hydroxyapatite for the repair of osseous defects prior to endosseous implant surgery. J Oral Implantol 15 : 186, 1989.
10 Cai K, Yao K, Lin S et al : Poly(D, L-lactic acid) surfaces modified by silk fibroin : effects on the culture of osteoblast in vitro. Biomaterials 23 : 1153, 2002.   DOI   ScienceOn
11 Postlethwait RW : Tissue reaction to surgical sutures. In: Dumphy JE, van Winkle W, editors. Repair and regeneration. New York, McGraw-Hill, 1969. p. 263.
12 Feldkamp LA, Goldstein SA, Parfitt AM et al : The direct examination of three-dimentional bone architectures in vitro by computed tomography. J Bone Miner Res 4 : 3,1989.
13 Kapadia RD, Stroup GB, Badger AM et al : Applications of micro-CT and MR microscopy to study pre-clinical models of osteoporosis and osteoarthritis. Technel Health Care 6 : 361, 1998.
14 Verna C, Bosch G, Dalstra M et al : Healing patterns in calvarial bone defects following guided bone regeneration in rats. A miro-CT scan analysis. J Clin Periodontol 29 : 865, 2002.   DOI   ScienceOn
15 Balto K, Muller R, Garrington DC et al : Quantification of periapical bone destruction in mice by micro-computed tomography. J Dent Res 79 : 35, 2000.   DOI   ScienceOn
16 Giesen EB, van Eijden TM : The three-dimentional cancellous bone architecture of the human mandibular condyle. J Dent Res 79 : 957, 2000.   DOI   ScienceOn
17 Murugan R, Ramakrishna S : Development of nanocomposites for bone grafting. Compos Sci Technol 65 : 2385, 2005.   DOI   ScienceOn
18 Zhao J, Zhang Z, Wang S et al : Apatite-coated silk fibroin scaffolds to healing mandibular border defects in canines. Bone 45 : 517, 2009.   DOI   ScienceOn
19 Rossitch E Jr, Bullard DE, Oakes WJ : Delayed foreignbody reaction to silk sutures in pediatric neurosurgical patients. Childs Nerv Syst 3 : 375, 1987.   DOI   ScienceOn
20 Soong HK, Kenyon KR : Adverse reactions to virgin silk sutures in cataract surgery. Ophthalmology 91 : 479, 1984.   DOI
21 Li C : Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 27 : 3115, 2006.   DOI   ScienceOn
22 Wang X, Kim HJ, Xu P et al : Biomaterial coating by stepwise deposition of silk fibroin. Langmuir 21 : 11335, 2005.   DOI   ScienceOn
23 Bai J, Ma T, Chu W et al : Regenerated spider silk as a new biomaterial for MEMS. Biomed Microdevices 8 : 317, 2006.   DOI   ScienceOn
24 Stitzel J, Liu J, Lee SJ et al : Controlled fabrication of a biological vascular substitute. Biomaterials 27 : 1008, 2006.
25 Gosline JM, Demont ME, Denny MW : The structure and properties of spider silk. Endeavour 10 : 37, 1986.   DOI
26 Craig CL, Riekel C : Comparative architecture of silks, fibrous proteins, and their encoding genes in insects and spiders. Comp Biochem Physiol B Biochem Mol Biol 135 : 721, 2003.   DOI   ScienceOn
27 Lorena M, Robert F : Silk implants for the healing of critical size bone defect. Bone 37 : 688, 2005.   DOI   ScienceOn
28 Hirano Y, Mooney DJ : Peptide and protein presenting materials for tissue engineering. Adv Mater 16 : 17, 2004.   DOI   ScienceOn
29 Dal Pra I, Freddi G, Minic J et al : De novo engineering of reticular connective tissue in vivo by silk fibroin nonwoven materials. Biomaterials 26 : 1987, 2005.   DOI   ScienceOn
30 Kaplan DL, Fossey S, Viney C et al : Self-organization (assembly) in biosynthesis of silk fibers-a hierarchical problem. In : Aksay IA, Baer E, Sarikaya M, Tirrell DA, editors. Hierarchically structured materials. Mater Res Symp Proc 255 : 19, 1992.
31 Bucholz RW, Carlton A, Holmes RE : Hydroxyapatite and tricalcium phosphate bone graft substitute. Orthop Clin North Am 18 : 323, 1987.
32 Laurencin CT, Ambrosio AMA, Borden MD et al : Tissue engineeIing : orthopedic applications. Annu Rev Biomed Eng 01 : 19, 1999.   DOI   ScienceOn
33 Radin SR, Ducheyne P : Effect of bioactive ceramic composition and structure on in vitro behavior. III : porous versus dense ceramics. J Biomed Mater Res 28 : 1303, 1994.   DOI   ScienceOn
34 Redey SA, Razzouk S. Rey C et al : Osteoclast adhesion and activity on synthetic hydroxyapatite, carbonated hydroxyapatite, and natural calcium carbonate : relationship to surface energies. J Biomed Mater Res 45 : 140, 1999.   DOI   ScienceOn
35 Prokic B, Carranza FA, Kenny EB et al : Comparative clinical study of porous hydroxyapatite and decalcified freeze-dried bone in human periodontal defects. J Periodontol 61 : 399, 1990.   DOI
36 Papay FA, Morales L, Ahmed OF et al : Comparison of ossification of demineralized bone, hydroxyapatite, Gelfoam, and bone wax in cranial defect repair. J Craniofac Surg 7 : 347, 1996.   DOI   ScienceOn