Browse > Article

THE HISTOLOGIC STUDY OF BONE HEALING AFTER HORIZONTAL RIDGE AUGMENTATION USING AUTO BLOCK BONE GRAFT  

Oh, Jae-Kwen (Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental School)
Choi, Byung-Jun (Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental School)
Lee, Baek-Soo (Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental School)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.31, no.3, 2009 , pp. 207-215 More about this Journal
Abstract
Purpose: The aim of the present study is to evaluate the long term bone healing after horizontal ridge augmentation using auto block bone graft for implant installation timing. Materials and Methods: Five Beagle dogs(which were 14 months old and weighted approximately 10kg). In surgery 1(extraction & bone defect), premolars(P2, P3,P4) were extracted and the buccal bone plate was removed to create a horizontally defected ridge. After three months healing, in surgery 2(ridge augmentation). Auto block bone grafts from the mandibular ramus were used in filling the bone defects were fixed with stabilizing screws. The following fluorochrome labels were given intravenously to the beagle dogs: oxytetracycline 1week after the surgery, alizarin red 4 weeks after the surgery, calcein blue 8 weeks after the surgery. The tissue samples were obtained from the sacrificed dogs of 1, 4, 8, 12, 16 weeks after the surgery. Non-decalcified sections were prepared by resin embedding and microsection to find thickness of $10{\mu}m$ for the histologic examination and analysis. Results: 1. We could achieve the successful reconstruction of the horizontal bone defect by auto block bone graft. The grafted bone block remained stable morohologically after 16 weeks of the surgery. 2. In the histologic view. We observed osteoid tissue from the sample $4^{th}$ week sample and active capillary reconstruction in the grafted bone from the $12^{th}$ week sample. Healing procedures of auto bone grafts were compared to that of the host bone. 3. Bone mineralization could be detected from the $8^{th}$ week sample. 4. Fluorochrome labeling showed active bony changes and formation at the interface of the host bone and the block graft mainly. Bony activation in the grafted bone could be seen from the $4^{th}$ week samples. Conclusions: Active bone formation and remodeling between the grafted bone and host bone can be seen through the revascularization. After the perfect adhesion to host bone, Timing of successful implant installation can be detected through the ideal ridge formation by horizontal ridge augmentation.
Keywords
Auto block bone; Horizontal bone augmentation; Bone labeling;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Urist MRB : Formation by autoinduction.Science 150 : 893, 1965   DOI   PUBMED   ScienceOn
2 Kostopoulos L, Karring T : Augmentation of the mandible using guided tissue regeneration. Clin Oral Impl Res 5 : 75, 1994   DOI   ScienceOn
3 Dahlin C, Lindhe A, Gottlow J et al : Healing of bone defects by guided tissue regeneration. Plastic Reconst Surg 81 : 672, 1988   DOI   ScienceOn
4 Araujo MG, Sonohara M, Hayacibara R et al : Lateral ridge augmentation by the use of grafts comprised of autologous bone or a biomaterial. An experiment in the dog. J Clin Periodontol 29 : 1122, 2002   DOI   ScienceOn
5 Stoelinga PJ, Tideman H, Berger JS : Interpositional bone graft augmentation of the atrophic mandible: a preliminary report. J Oral Surg 36 : 30, 1978
6 Jensen J, Sindet PS : Autogenous mandibular bone grafts and osseointegrated implants for reconstruction of the severely atrophic maxilla. J Oral Maxillofac Surg 49 : 1277, 1991   DOI   ScienceOn
7 Kim YG, Kim SG, Lee BK : Bone graft and Implant Vol 1. Bone Biology anBone graft materials, Narae Publishing, Inc. 13, 2005
8 Phillips JH, Rahn BA : Fixation effects on membranous and endochondral onlay bone graft revascularization and bone deposition. Plast Reconstr Surg 85 : 891, 1990   DOI   ScienceOn
9 Atwood D : Post extraction changes in adult mandible as illustrated by microradiograph of midsagittal section and serial cephalometric roetgengrams. J Prosthet Dent 13 : 810, 1963   DOI
10 Kerwin SC, Lewis DD, Elkins AD : Bone grafting and banking. Compen Con Edu Pra Veteri 3 : 1558, 1991
11 Carlsson H, Thilander H, Hedegard B : Histologic changes in the upper alveolar process after extractions with or without insertion of an immediate full denture. Acta Odontol Scand 25 : 21, 1967   DOI   ScienceOn
12 Chiapasco M, Abati S : Clinical outcome of autogenous bone blocks or guided bone regeneration with e-PTFE membranes for the reconstructionof narrow edentulous ridge. Clin Oral Impl Res 10 : 278, 1999   DOI   ScienceOn
13 Donos N, Karring T : Long-term stability of autogenous bone grafts following combined application with guided bone regeneration. Clin Oral Implants Res 19 : 80, 2005   DOI   ScienceOn
14 Axhausen W : Osteogenic phase of regeneration of bone : A historical and experimental study. J Bone Joint Surg 38 : 593,1956
15 Park YG : Bone Histomorphometry. Kyung-Hee Pub 43, 2004
16 Park HW, Lee BS : A histologic study of bone formation according to different cryoprotectants during cryopreservation of bone. J Korean Assoc Oral Maxillofac Surg 31 : 228, 2005
17 Ilizarov GA : The tension-stress effect on the genesis and growth of tissues : Part I. The influence of stability of fixation and soft tissue preservation. Clin Orthop 238 : 249, 1989
18 Roccuzzo M, Ramieri G, Spada MC et al : Vertical alveolar ridge augmentation by means of a titanium mesh and autogenous bone grafts. Clin Oral Impl Res 15 : 73, 2004   DOI   ScienceOn
19 Decker B, Bartels H : Relationships between endothelial cells, pericytes, and osteoblasts during bone formation in the sheep femur following implantation of tricalciumphosphate-ceramic. Anatomic Record 242 : 310, 1995   DOI   ScienceOn
20 Thomas A, David LC, Daniel B : Lateral ridge augmentation using defferent bone fillers and barrier membrane application. Clin. Oral Impl Res 12 : 260, 2001   DOI   ScienceOn
21 Donos N, Kostopoulos L, Karring T : Augmentation of the rat jaw with autogenic cortico - cancellous bone grafts and guided tissue regeneration. Clin Oral Impl Res 13 : 192, 2002   DOI   ScienceOn
22 Schenk RK, Buser D, Hardwick WR et al : Healing pattern of bone regeneration in membrane-protected defects. Int J Oral Maxillofac Implants 9 : 13, 1994
23 Parfitt AM : The physiologic and clinical significance of bone histomorphometric data. CRC Pres 143, 1983
24 Oh JH, Kim JD, Kim YG et al : Implant Restoration after Alveolar Distraction o Anterior Mandible : A Case Report, The Journal of Kyung Hee University Medical Center 21/1 : 91, 2005
25 Albrektsson T : The healing of autogenous grafts varying degrees of surgical trauma. J Bone Joint Surg 14 : 1, 1980
26 Buser D, Dula K, Hess D et al : Localized ridge augmentation with autografts and barrier membranes. J Periodontol 19 : 151, 2000
27 Kim YG, Yoon BW, Ryu DM et al : A study on the bone formation of graft material containing demineralized bone matrix with a simultaneous installation of implant. J Korean Assoc Oral Maxillofac Surg 31 : 481, 2005
28 Buser D, Dula K, Hirt HP et al : Lateral ridge augmentation using autografts and barrier membranes. A clinical study in 40 partially edentulous patients. Int J Oral Maxillofac Surg 54 : 420, 1996   DOI   ScienceOn
29 Mori S, Burr DB : Increased intracortical remodeling following fatigue damage. Bone 14 : 103, 1993   DOI   ScienceOn
30 Burchardt H : The biology of bone graft repair. Clin Orthop Rel Res 174 : 28, 1983
31 Marx R E : Clinical applications of Bone biology to mandibular and maxillary reconstructions. Clin Plas Surg 21 : 377, 1994
32 Arun KG : Bone & Biology, Harvesting, Grafting for Dental Implants. Rationale and Clinical Applications. Quintessence Pub 12, 2004
33 Matthias AWM, Japp CM, Hans MF et al : Incorporation of particulated bone implants in the facial skeleton. Biomaterials 20 : 2029, 1999   DOI   ScienceOn
34 Younger EM, Chapman MW : Morbidity at bone graft donor sites. J Orthop Trau 3 : 192, 1989   DOI
35 Mashiba T, Hui S, Turner CH et al : Bone remodeling at the iliac crest can predict the changes in remodeling dynamics, micredamage accumulation, end mechanical properties in the lumbar vertebrae of dogs. Calcif Tissue Int 77 : 180, 2005   DOI   ScienceOn
36 Lee TC, Moshin S, Taylor D et al : Detecting microdamage in bone. J Anat 203 : 161, 2003   DOI   ScienceOn
37 Fumiaki M, Kunikazu T, Koichi W : Widespread calcium deposits, as detected using the alizarin red S technique, in the nervous system of rats treated with dimethyl mercury. Neuropathology 20 : 210, 2000   DOI   ScienceOn
38 Evian CI, Rosenberg ES, Coslet JG et al : The osteogenic activity of bone removed from healing extraction sockets in humans. J Clin Periodontol 53 : 81, 1982   DOI