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http://dx.doi.org/10.7783/KJMCS.2020.28.4.276

Improvement Effect of Non-alcoholic Fatty Liver Disease by Curcuma longa L. Extract  

Lee, Young Seob (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Dae Young (Department of Herbal Crop Research, NIHHS, RDA)
Kwon, Dong Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University)
Kang, Ok Hwa (Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Publication Information
Korean Journal of Medicinal Crop Science / v.28, no.4, 2020 , pp. 276-286 More about this Journal
Abstract
Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease associated with multiple metabolic disorders. The medicinal plant Curcuma longa L. is widely distributed in Asia and has been used to treat a spectrum diseases in clinical practice. To date, there are inadequate reports of the effects of C. longa 50% EtOH extract (CE) on NAFLD. Therefore, in this study, we evaluate the CE on an NAFLD animal and elucidate the mechanism of action. Methods and Results: C57BL/6J mice fed a methionine-choline deficient diet (MCD) were treated with CE or milk thistle, and changes in inflammation and stetosis were assessed. Experimental animals were divided into six group (n = 10); Normal, MCD, MCD + CE 50 mg/kg/day (CE 50), MCD + CE 100 mg/kg/day (CE 100), MCD + CE 150 mg/kg/day (CE 150), and the Control, MCD + Milk thistle 150 mg/kg/day (MT 150). Body weight, liver weight, liver function, and histological changes were assessed in experimental animals. Quantitative real-time polymerase chain reaction and western blot analyses were performed on samples collected after 4 weeks of treatment. We observed that CE administration improved MCD-diet-induced lipid accumulation, and triglyceride (TG) and total cholesterol (TC) levels in serum. Treatment with CE also decreased hepatic lipogenesis through modulation of the sterol regulatory element binding protein-1 (SREBP-1), CCAAT-enhancer binding protein α (C/EBPα), fatty acid synthase (FAS), and peroxisome proliferator-activated receptor γ (PPARγ) expresion. In addition, the use of CE increased adenosine monophosphate-activated protein kinase (AMPK) phosphorylation and inhibited the up-regulation of toll-like receptor (TLR)-2 and TLR-4 signaling and the production of inflammatory mediators. Conclusions: In this report, we observed that CE regulated lipid accumulation in an MCD dietinduced NAFLD model by decreasing lipogenesis. These data suggeste that CE could effectively protect mice against MCD-induced NAFLD, by inhibiting the TLR-2 and TLR-4 signaling cascades.
Keywords
Curcuma longa L. (C. longa); Non-alcoholic Fatty Liver Disease; Methionine Choline Deficient-diet; Adenosine Monophosphate-activated Protein Kinase; Toll-like Receptor-2; Toll-like Receptor-4;
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Times Cited By KSCI : 2  (Citation Analysis)
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