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http://dx.doi.org/10.7783/KJMCS.2018.26.5.391

Comparison of Anti-oxidative and Anti-inflammatory Effect of Atractylodes Interspecific Hybrid Cultivar Roots  

Jeong, Hyeon Soo (Department of Herbal Crop Research, NIHHS, RDA)
Jeong, Jin Tae (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA)
Park, Chun Geon (Department of Herbal Crop Research, NIHHS, RDA)
Choi, Je Hun (Department of Herbal Crop Research, NIHHS, RDA)
Jang, Gwi Yeong (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Jang Wook (Department of Herbal Crop Research, NIHHS, RDA)
Chang, Jae Ki (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Seung Eun (Department of Herbal Crop Research, NIHHS, RDA)
Publication Information
Korean Journal of Medicinal Crop Science / v.26, no.5, 2018 , pp. 391-400 More about this Journal
Abstract
Background: Atractylodes radix is a well-known medicinal crop having many physiological effects. This study was conducted to select useful Atractylodes japonica ${\times}$ Atractylodes macrocephala (AJM) cultivars by comparing anti-oxidative and anti-inflammatory efficacies. Methods and Results: Seven extracts from AJM cultivars were used to treat lipopolysacchride (LPS)-treated BV2 cells, and the effects on cell viability and inhibition on reactive oxygen species (ROS) and nitric oxide (NO) production were analyzed. In vitro scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxynitrite ($NOO^-$) radicals were also investigated. Contents of total phenol, atractylenolide I, and atractylenolide III in the AJM extracts were measured using high performance liquid chromatography (HPLC) or spectrophotometry. The experiments show that none of the seven extracts was cytotoxic above 89.2% at $20-250{\mu}g/m{\ell}$. Extracts of Gowon, Dawon, Sangchul, and Huchul inhibited ROS generation in a dose-dependent manner, and Sangchul extract showed the highest inhibition on ROS production. All the AJM extracts showed effective inhibitory activity after on NO release in the LPS-treated BV2 cells, and Sangchul extract showed the highest activity. Sangchul extract had the most potent scavenging activities for $NOO^-$ and had some DPPH radical scavenging effect. Sangchul extract also had the highest content at total phenol and atractylenolide I content. Atractylenolide III was not detected in the AJM extracts. Conclusions: The results suggested that Sangchul was the most useful anti-oxidative and anti-inflammatory resource among the AJM cultivars.
Keywords
Atractylodes; Cultivars; Anti-inflammation; Anti-oxidation;
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