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http://dx.doi.org/10.7783/KJMCS.2018.26.4.302

Pathgenicity on Ginseng and Sequence Assays of Ilyonectria radicicola Isolated from Chestnut Rhizosphere Soils  

Seo, Mun Won (Department of Applied Biology, Chungnam National University)
Song, Jeong Young (Department of Applied Biology, Chungnam National University)
Kim, Sun Ick (Ginseng and Medicinal Crop Experiment Station, CNARES)
Oh, Sang Keun (Department of Applied Biology, Chungnam National University)
Kim, Hong Gi (Department of Applied Biology, Chungnam National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.26, no.4, 2018 , pp. 302-307 More about this Journal
Abstract
Background: A soil-borne pathogenic fungus, Ilyonectria radicicola (Cylindrocarpon destructans) causes root rot on ginseng (Panax ginseng C. A. Meyer) and is known to attack many other plants. The Nectria/Neonectria radicicola complex has been renamed as the I. radicicola complex after analysis of its multi-gene relatedness and morphological characteristics. The fungi in this complex have been reclassified into 16 species under the genus Ilyonectria based on characteristics analysis Methods and Results: To obtain useful data from the Korean ginseng root rot, I. radicicola was isolated from the rhizosphere soils of the chestnut tree. They were identified through a pathogenicity test and a survey of the morphological features. The existence of I. radicicola in soil samples was confirmed by PCR detections using nested PCR with species-specific primer sets. These were subsequenctly isolated on semi-selective media from PCR-positive soils. Genetic analysis of the I. radicicola complex containing these pathogens was done by comparing the DNA sequences of the histone h3 region. These isolates originating from the rhizosphere soils of chestnut constituted a clade with other closely related species or I. radicicola isolates originating from ginseng or other host plants, respectively. Additionally, the pathogenicity tests to analyze the characteristics of these I. radicicola isolates revealed that they caused weakly virulent root rot on ginseng. Conclusions: This is the first study reporting that I. radicicola isolates from chestnut rhizosphere soils can attack ginseng plant in Korea. Thus, these results are expected to provide informations in the selection of suitable fields for ginseng cultivation.
Keywords
Ilyonectria radicicola; Panax ginseng C. A. Meyer; Chestnut Rhizosphere Soils; Ginseng Root Rot; Weakly Virulence;
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1 The Korean Society of Plant Pathology(KSPP). (2009). List of plant diseases in Korea. The Korean Society of Plant Pathology. Seoul, Korea. p.91-288.
2 Matuo T and Miyazawa Y. (1984). Scientific name of Cylindrocarpon sp. causing root rot of ginseng. Japanese Journal of Phytopathology. 50:649-652.   DOI
3 Punja ZK. (1997). Fungal pathogens of American ginseng(Panax quinquefolius) in British Columbia. Canadian Journal of Plant Pathology. 19:301-306.   DOI
4 Reeleder RD, Roy R and Capell B. (2002). Seed and root rots of ginseng(Panax quinquefolius L) caused by Cylindrocarpon destructans and Fusarium spp. Journal of Ginseng Research. 26:151-158.   DOI
5 Seifert KA, McMullen CR, Yee D, Reeleder RD and Dobinson KF. (2003). Molecular differentiation and detection of ginsengadapted isolates of the root rot fungus Cylindrocarpon destructans. Phytopathology. 93:1533-1542.   DOI
6 Seo MW, Song JY, Kang KH, Park SY, Kim SI and Kim HG. (2017). Virulence assays and genetic reclassification to assess the pathogenicity of Cylindrocarpon destructans isolated from peony in ginseng. The Korean Journal of Mycology. 45:132-138.
7 Seo MW. (2009). Analysis of characteristics for Korean ginseng root rot pathogen(Cylindrocarpon destructans) and development of new diagnostic molecular marker. Master Thesis. Chungnam National University. p.28-35.
8 Seo MW. (2017). Reclassification and disease occurrence foctor in first planted field of Korean ginseng root rot pathogen population(Cylindrocarpon destructans) and practical disease diagnosis. Ph. D. Thesis. Chungnam National University. p.28-34.
9 Singleton LL, Mihail JD and Rush CM. (1992). Methods for research on soilborne phytopathogenic fungi. In Jeanne M. et al. APS Press. St. Paul. MN, USA. p.140-141.
10 Tamura K, Stecher G, Peterson D, Filipski A and Kumar S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 30:2725-2729.   DOI
11 Jang CS. (2005). Development of diagnostic system for root rot disease of ginseng caused by Cylindorcarpon destructans using nested PCR from soil. Master Thesis. Chungnam National University. p.7-10.
12 Crous PW, Groenewald JZ, Risède JM, Simoneau P and Hywel-Jones NL. (2004). Calonectria species and their Cylindrocladium anamorphs: Species with sphaeropedunculate vesicles. Studies in Mycology. 50:415-430.
13 Doyle JJ. (1990). Isolation of plant DNA from fresh tissue. Focus. 12:13-15.
14 Hamelin RC, Berube P, Gignac M and Bourassa M. (1996). Identification of root rot fungi in nursery seedlings by nested multiplex PCR. Applied and Environmental Microbiology. 62:4026-4031.
15 Jun NJ. (2008). Study on new host range and genetic diversity for ginseng root rot pathogen, Cylindrocarpon destructans in Korea. Master Thesis. Chungnam National University. p.27-40.
16 Kang SW, Yeon BY, Hyeon GS, Bae YS, Lee SW and Seong NS. (2007). Changes of soil chemical properties and root injury ratio by progress years of post-harvest in continuous cropping soils of ginseng. Korean Journal of Medicinal Crop Science. 15:157-161.
17 Lee SW, Lee SH, Park KH, Jang IB, Jin ML and Seo MW. (2017). Effect of crop rotation system on soil chemical properties and ginseng root rot after harvesting ginseng. Korean Journal of Medicinal Crop Science. 25:244-251.   DOI
18 Lee SW, Lee SH, Park KH, Jin ML, Jang IB and Kim KH. (2015). Inhibition effect on root rot disease of Panax ginseng by crop cultivation in soil occurring replant failure. Korean Journal of Medicinal Crop Science. 23:223-230.   DOI
19 Cabral A, Groenewald JZ, Rego C, Oliveira H and Crous PW. (2012). Cylindrocarpon root rot: Multi-gene analysis reveals novel species within the Ilyonectria radicicola species complex. Mycological Progress. 11:655-688.   DOI
20 Lee SW, Park KH, Lee SH, Jang IB and Jin ML. (2016). Crop rotation in paddy soil exhibiting crop failure following replanting: Effect on soil chemical properties, soil microbial community and growth characteristics of 2-year-old ginseng. Korean Journal of Medicinal Crop Science. 24:294-302.   DOI
21 Cho DH, Park KJ, Yu YH, Ohh SH and Lee HS. (1995). Rootrot development of 2-year old ginseng(Panax ginseng C. A. Meyer) caused by Cylindrocarpon destructans(Zinssm.) Scholten in the continuous cultivation field. Korean Journal of Ginseng Science. 19:175-180.
22 Choi SY, Park KS, Kim KJ and Kim JC. (2004). Occurrence and control of black root rot of peony(Paeonia lactiflora) on continuous cropping. Research in Plant Disease. 10:268-271.   DOI
23 Booth C. (1966). The genus Cylindrocarpon. Mycological Papers. 104:1-56.