Browse > Article
http://dx.doi.org/10.7783/KJMCS.2018.26.2.170

Changes in Non-saponin Fatty Acid Content and Increases in Inhibitory Activities of Collagenase and Elastase by Treatment with Saccharomyces cerevisiae of the Supercritical Fluid Extracted Oil of the Adventitious Roots Culture of Wild Mountain Ginseng  

Kim, Chul Joong (Research Institute of Biotechnology, HwajinBioCosmetic)
Shim, Jae Kwon (Research Institute of Biotechnology, HwajinBioCosmetic)
Kwon, Kyung Cheol (Research Institute of Biotechnology, HwajinBioCosmetic)
Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
Choi, Seon Kang (Department of Agricultural Life Sciences, Kangwon National University)
Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University)
Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic)
Publication Information
Korean Journal of Medicinal Crop Science / v.26, no.2, 2018 , pp. 170-180 More about this Journal
Abstract
Background: To obtain useful cosmetic resources, this study aimed to determine the non-saponin fatty acid and inhibitory activities of collagenase and elastase by treatment of Saccharomyces cerevisiae in supercritical fluid extracted oil of the adventitious root culture of wild mountain ginseng. Methods and Results: We performed supercritical fluid extraction at various conditions such as pressure, temperature, time, and use of co-solvents, unlike the n-hexane extraction for the adventitious roots culture of wild mountain ginseng. The non-saponin-fatty acid obtained from the oil of the adventitious roots culture was incresed by treatment with S. cerevisiae. The supercritical fluid extraction was conducted using gas chromatography. Non-saponin-fatty acid content, in the oil of adventitious roots culture of wild mountain ginseng treated with S. cerevisiae for 2 days were three times higher than that in the control. In addition, the oil of the adventitious roots culture treated with S. cerevisiae was investigated for the anti-wrinkle effect by using collagenase and elastase. The oil of adventitious roots culture treated with S. cerevisiae exhibited higher collagenase and elastase inhibitory activities than those in the control. Conclusions: Supercritical fluid extracted oil of the adventitious roots culture of wild mountain ginseng treated with S. cerevisiae was found to have decreased ratio of saturated fatty acids and incresed ratio and content of unsaturated fatty acids increased. Furthermore, it showed anti-wrinkle effects in vitro.
Keywords
Panax ginseng C. A. Meyer; Saccharomyces cerevisiae; Mountain Wild Ginseng Adventitious Roots Culture; Non-saponin Fatty Acid; Supercritical Fluid Extraction;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Kim CJ, Seong ES, Yoo JH, Lee JG, Kim NJ, Choi SK, Lim JD and Yu CY. (2016). Biological activity of Panax ginseng C. A. Meyer culture roots fermented with microorganisms. Korean Journal of Medicinal Crop Science. 24:191-197.   DOI
2 Kim JB, Kim SH, Park SY and Chung DK. (2010). Effects of Korean mountain ginseng leaves and its active constituents on scopolamine-induced amnesia in mouse. Journal of Oriental Neuropsychiatry. 21:125-144.
3 Kim SH, Jung H, Shin YC and Ko SG. (2008). Research of traditional herbal medicines for anti-aging, inhibition effect of wrinkle and whitening effect in the skin. Korean Journal of Oriental Physiology and Pathology. 22:691-698.
4 Kim TH, Jeon SH, Hahn EJ, Paek KY, Park JK, Youn NY and Lee HL. (2009). Effects of tissue-cultured mountain ginseng(Panax ginseng CA Meyer) extract on male patients with erectile dysfunction. Asian Journal of Andrology. 11:356-361.   DOI
5 Lee BJ, Kim JW, Ji EY, Yun SY, Lee SM and Lew JH. (2009). Anxiolytic and antidepressive effect of non-saponin fraction of Korean red ginseng. Korea Journal of Herbology. 24:143-148.
6 Lee JM, Lee HJ, Kang SB and Park WJ. (2016a). Fatty acid desaturases, polyunsaturated fatty acid regulation, and biotechnological advances. Nutrients. 8:23.   DOI
7 Lee MJ, Kim KH and Bae JO. (2006). The effects of supercritical carbon dioxide on the extraction of perilla oil. Journal of the Korean Society of Food Science and Nutrition. 35:1439-1443.   DOI
8 Lee MY, Kim BA and Yang JC. (2016b). Effects of extracts derived from red ginseng residue on antioxidant activity and elastase inhibition. Journal of the Korean Oil and Applied Science. 33:658-666.
9 Lee SM, Yun JH and Chun BS. (2011b). Fatty acid composition and oxidative properties of anchovy oil extracted by supercritical carbon dioxide. Clean Technology. 17:266-272.
10 Lee YS, Im DH, Yang JC, Noh DS, Kim KI, Oh SK, Choi KC and Cha YH. (2011a). Study on the qualitative discrimination of white, red, and black ginseng extract. The Korean Journal of Food and Nutrition. 24:138-143.   DOI
11 Martin CE, Oh CS, Kandasamy P, Chellapa R and Vemula M. (2002). Yeast desaturases. Biochemical Society Transactions. 30:1080-1082.   DOI
12 Miquel MF and Browse JA. (1994). High-oleate oilseeds fail to develop at low temperature. Plant Physiology. 106:421-427.   DOI
13 Mori M, Ikeda N, Kato Y, Minamino M and Watabe K. (2002). Inhibition of elastase activity by essential oils in vitro. Journal of Cosmetic Dermatology. 1:183-187.   DOI
14 Niemann H. (2004). Transgenic pigs expressing plant genes. Proceedings of the National Academy of Sciences of the United States of America. 101:7711-7212.   DOI
15 Park JW, Jung MS and Park CM. (2016). Cosmetic composition comprising oil of cultured root of wild ginseng. Korea. Patent. 10-2016-0108917.
16 Park SJ, Yoo SM and Kim YE. (2012). Nutritional characteristics and screening of biological activity of cultured wild ginseng roots. Korean Journal of Food and Nutrition. 25:729-736.   DOI
17 Rennert B and Melzig MF. (2002). Free fatty acids inhibit the activity of clostridium histolyticum collagenase and human neutrophil elastase. Planta Medica. 68:767-769.   DOI
18 Petrie JR, Shrestha P, Belide S, Kennedy Y, Lester G, Liu Q, Divi UK, Mulder RJ, Mansour MP, Nichols PD and Singh SP. (2009). Metabolic engineering Camelina sativa with fish oil-like levels of DHA. PLOS ONE. 9:1-8.
19 Qiu X, Hong H, Datla N, MacKenzie SL, Taylor DC and Thomas TL. (2002). Expression of borage ${\Delta}6$ desaturase in Saccharomyces cerevisiae and oilseed crops. Canadian Journal of Botany. 80:42-49.   DOI
20 Rebole A, Rodriguez ML, Ortiz LT, Alzueta C, Centeno C, Viveros A, Brenes A and Arija I. (2006). Effect of dietary higholeic acid sunflower seed, palm oil and vitamin E supplementation on broiler performance, fatty acid composition and oxidation susceptibility of meat. British Poultry Science. 47:581-591.   DOI
21 Shin CS, Lee DH, Kim SH, Shin MH, Jeong CH and Shim KH. (2010). Ginsenoside contents and antioxidative activities from red ginseng treated with high hydrostatic pressure. Journal of Agriculture and Life Science. 44:133-140.
22 Shin MH. (2001). Study of mountain ginseng adventitious culture and application. Journal of the Society of Cosmetic Scientists of Korea. 27:45-56.
23 Shon MS, Song JH, Kim JS, Jang HD and Kim GN. (2013). Anti-oxidant activity of oil extracted from Korean red ginseng and its moisturizing function. Korean Journal of Aesthetic Society. 11:489-494.
24 Choi ME, Jeon BK, Kim DS, Mun YJ and Woo WH. (2009). A study on application for beauty food of mixture of Korean red ginseng and Fagopyrum esculentum: Anti-oxidative effect and collagenase inhibitory activity. Herbal Formula Science. 17:153-162.
25 Tsuji N, Moriwaki S, Suzuki Y, Takema Y and Imokawa G. (2001). The role of elastases secreted by fibroblasts in wrinkle formation: Implication through selective inhibition of elastase activity. Photochemistry and Photobiology. 74:283-290.   DOI
26 Wunsch E and Heidrich HG. (1963). Zur quantitativen bestimmung der kollagenase. Biological Chemistry. 333:149-151.
27 Yoo JH, Choi JH, Kang BJ, Jeon MR, Lee CO, Kim CH, Seong ES, Heo K, Yu CY and Choi SK. (2017). Antioxidant and tyrosinase inhibition activity promoting effects of perilla by the light emitting plasma. Korean Journal of Medicinal Crop Science. 25:37-44.   DOI
28 Youn JS, Shin SY, Wu Y, Hwang JY, Cho JH, Ha YG, Kim JK, Park MJ, Lee SH, Kim TH and Kim TW. (2012). Antioxidant and anti-wrinkling effects of Aruncus dioicus var. kamtschaticus extract. Korean Journal of Food Preservation. 19:393-399.   DOI
29 Yu HS. (2014). Effect of ethanol extract of Rosa rugosa Thunb. on hypopigmentation and antioxidant activity. Ph.D. Thesis. University of Wonkwang. p.1-65.
30 Cannell RJP, Kellam SJ, Owsianka AM and Walker JM. (1988). Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Medica. 54:10-14.   DOI
31 Daum G and Vance JE. (1997). Import of lipids into mitochondria. Progress in Lipid Research. 36:103-130.   DOI
32 de Witt DL, Rollins TE, Day JS, Gauger JA and Smith WL. (1981). Orientation of the active site and antigenic determinants of prostaglandin endoperoxide synthase in the endoplasmic reticulum. Journal of Biological Chemistry. 256:10375-10382.
33 Gillis CN. (1997). Panax ginseng pharmacology: A nitric oxide link? Biochemical Pharmacology. 54:1-8.   DOI
34 Hur CG, Park DS, Park HN, Lee JS and Lee HY. (2011). Method for producing omega fatty acid-containing extract from plant using supercritical carbon dioxide extraction. USA. Patent. 13,696,060.