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http://dx.doi.org/10.7783/KJMCS.2018.26.2.157

A 26-Week Repeated Oral Dose Toxicity Test and a 4-Week Recovery Test of Cassia tora L. Water Extract in Sprague-Dawley Rats  

Nho, Jong Hyun (National Development Institute of Korean Medicine)
Lee, Mu Jin (National Development Institute of Korean Medicine)
Jung, Ho Kyung (National Development Institute of Korean Medicine)
Jang, Ji Hun (National Development Institute of Korean Medicine)
Sim, Mi Ok (National Development Institute of Korean Medicine)
Jang, Min Cheol (Heanam Natural Farming Association Corporation)
Yong, Ju Hyun (Heanam Natural Farming Association Corporation)
Seo, Heung Sik (Korea Testing and Research Institute)
An, Byeong Kwan (National Development Institute of Korean Medicine)
Kim, Jong Choon (College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University)
Cho, Hyun Woo (National Development Institute of Korean Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.26, no.2, 2018 , pp. 157-169 More about this Journal
Abstract
Background: Cassia tora L., an annual or perennial plant of the Fabaceae family, is traditional medicine with various biological activities, including anti-constipation and, anti-inflammation. Chemical compounds such as anthraquinone glycoside and naphthalene derivatives have been isolated from this plant. Cassia tora L. is a common contaminant of agricultural commodities, but is toxic to cattle and poultry. Methods and Results: To investigate the potential toxicity, Cassia tora L. aqueous extract (CO) was administered orally to rats for 26 weeks at 0 (control), 300, 1,500 and 3,000 mg/kg/day (n = 10 for male rats for each dose). The positive control comprised animals orally administered anthraquinone 100 mg/kg/day. There was no treatment-related mortality. An increase in the kidney weight was observed at 3,000 mg/kg/day of CO and anthraquinone 100 mg/kg/day. Macrophage infiltration in the colon was observed at CO 1,500 and 3,000 mg/kg/day and anthraquinone 100 mg/kg/day, but there were no significant toxicological changes in the incidence and severity of the finding. Conclusions: The oral no-observed-adverse-effect level (NOAEL) of CO was 3,000 mg/kg/day in male rats and no target organs were identified. In addition, 300 mg/kg was found to be the no-observed-effect level (NOEL) for systemic toxicity under the conditions of the study.
Keywords
Cassia tora L.; Herbal Medicine; Anthraquinone; Chronic Toxicity; NOEL (No Observed Effect Level);
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