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http://dx.doi.org/10.7783/KJMCS.2018.26.1.8

Anti-inflammatory and Immune Regulatory Effects of Aucklandia lappa Decne 70% Ethanol Extract  

Kim, Min Sun (Department of Food Biotechnology, Woosuk University)
Kim, Nam Seok (Department of Food Biotechnology, Woosuk University)
Kwon, Jin (Department of Prosthetics and Orthotics, Korea National University of Welfare)
Kim, Ha Rim (Department of Food Biotechnology, Woosuk University)
Lee, Da Young (Department of Food Biotechnology, Woosuk University)
Oh, Mi Jin (Korea Food Research Institute)
Kim, Hong Jun (Department of Korean Medicine, Woosuk University)
Lee, Chang Hyun (Department of Korean Medicine, Woosuk University)
Oh, Chan Ho (Department of Food Biotechnology, Woosuk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.26, no.1, 2018 , pp. 8-18 More about this Journal
Abstract
Background: This present study was conducted to evaluate the anti-inflammatory and immune regulatory effects of Aucklandia lappa Decne (AL). Methods and Results: We measured cytotoxicity, nitric oxide (NO) content, mRNA expression (iNOS, IL-1${\alpha}$, IL-$1{\beta}$, and TNF-${\alpha}$), protein expression (iNOS, COX-2, and $I{\kappa}B$) and phagocytic activity in RAW264.7 cells. Male BALB/c mice were fed 100 mg/kg AL (Aucklandia lappa Decneon 70% ethanol extract) and 250 mg/kg AL for 4 weeks; thereafter, we observed B/T or $CD4^+/CD8^+$ lymphocyte subpopulation change, and expression patterns of $CD4^+$ and $CD8^+$ lymphocytes by immunohistochemical staining in mouse splenocytes and/or thymocytes. To determine the experimental concentration of AL, cell viability was measured by MTT assay and tested at $12.5{\mu}g/m{\ell}$ or less. AL inhibited the levels of NO, lymphokine production (IL-$1{\beta}$, and TNF-${\alpha}$), and mRNA (iNOS, IL-1${\alpha}$, IL-$1{\beta}$, and TNF-${\alpha}$) and protein (iNOS, and COX-2) expression. Additionally, the levels of $I{\kappa}B$, phagocytic activity, and splenic and thymic T lymphocytes, especially $T_H$ and $T_C$ cells were significantly increased in AL administered mice. The immuno-reactive density of $CD4^+$ and $CD8^+$ lymphocytes was stronger in AL groups than in the normal group. AL stimulated NO, iNOS, and COX-2, and regulated IL-1${\alpha}$, IL-$1{\beta}$, TNF-${\alpha}$, and $I{\kappa}B$ in macrophages treated with LPS (lipopolysaccharide). In addition, AL increased the phagocytic activity of macrophages and the immunity of mouse T ($T_H$, and $T_C$) cells. Conclusions: These results suggested that AL might show anti-inflammatory activity via the suppression of various inflammatory markers and immuno-regulatory activity.
Keywords
Aucklandia lappa Decne; Anti-inflammatory Activity; Nitric Oxide; Phagocytic Activity; Protein Expression;
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