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http://dx.doi.org/10.7783/KJMCS.2017.25.4.201

Antimutagenic Effect of Mulberry Leaf Extract  

Lim, Bum Hyuk (Department of Industrial and System Engineering, KAIST)
Park, Chang Gyun (Department of Industrial Plant Science and Technology, Chungbuk National University)
Cho, Hyun Ki (Department of Industrial Plant Science and Technology, Chungbuk National University)
Lim, Heung Bin (Department of Industrial Plant Science and Technology, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.25, no.4, 2017 , pp. 201-208 More about this Journal
Abstract
Background: The present study was carried out to asses whether mulberry leaves (MLs) have the potential to inhibit the mutagenic effect of cigarette smoke condensates (CSCs). Methods and Results: ML powder was extracted with 70% ethanol, and a yield of 35.1% by weight was obtained. The 70% ethanol extract of ML was further extracted sequentially using diethyl ether, chloroform, butanol, dichloromethane and water. The crude 70% ethanol extract of MLs and its solvent fractions did not show any mutagenic effect when tested at concentrations up to 1 mg/plate against Salmonella typhimurium TA98. In contrast, the crude 70% ethanol extract showed an inhibitory activity against the mutagenicity of CSCs in the presence of S-9 mixture. Among the solvent fractions, the diethyl ether fraction showed the highest inhibitory activity, which increased in a dose-dependent manner, inhibiting mutagenesis by approximately 97.1% at a concentration of 1 mg/plate. Conclusions: In this study, we found that a crude 70% ethanol extract of MLs and the diethyl ether fraction themselves are potentially not mutagenic, but inhibit the mutagenic effect of CSCs.
Keywords
Morus alba L.; Salmonella typhimurium TA98; Antimutagenicity; Ames Test; Leaf Extract; Safety;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Jo SP, Kim JK and Lim YH. (2014). Antihyperlipidemic effects of stilbenoids isolated from Morus alba in rats fed a high-cholesterol diet. Food and Chemical Toxicology. 65:213-218.   DOI
2 Kang TH, Hur JY, Kim HB, Ryu JH and Kim SY. (2006). Neuroprotective effects of the cyanidin-3-O-beta-D-glucopyranoside isolated from mulberry fruit against cerebral ischemia. Neuroscience Letter. 391:122-126.   DOI
3 Khunakornvichaya A, Lekmeechai S, Pham PP, Himakoun W, Pitaksuteepong T, Morales NP and Hemstapat W. (2016). Morus alba L. stem extract attenuates pain and articular cartilage damage in the anterior cruciate ligament transection-induced rat model of osteoarthritis. Pharmacology. 98:209-216.   DOI
4 Kikuchi T, Nihei M, Nagai H, Fukushi H, Tabata K, Suzuki T and Akihisa T. (2010). Albanol A from the root bark of Morus alba L. induces apoptotic cell death in HL60 human leukemia cell line. Chemical and Pharmaceutical Bulletin. 58:568-571.   DOI
5 Kim JY, Ok HM, Kim J, Park SW, Kwon SW and Kwon O. (2015a). Mulberry leaf extract improves postprandial glucose response in prediabetic subjects: A randomized, double-blind placebo-controlled trial. Journal of Medicinal Food. 18:306-313.   DOI
6 Kim KH, Chung WS, Kim Y, Kim KS, Lee IS, Park JY, Jeong HS, Na YC, Lee CH and Jang HJ. (2015b). Transcriptomic anaysis reveals wound healing of Morus alba root extract by up-regulating karatin filament and CXCL 12/CXCR4 signaling. Phytotherapy Research. 29:1251-1258.   DOI
7 Kim MJ, Kim JS, Jeong DM, Ham SS and Yu CY. (2002a). Effect of antioxidant, antimutagenicity and anticancer of root extract from Ixeris denate Nakai. Korean Journal of Medicinal Crop Science. 10:222-229.
8 Kim MJ, Kim JS, Kang WH and Yeon KD. (2002b). Antimutagenic and cytotoxic effects of extracts of Kalopanax pictus Nakai endodermis. Korean Journal of Medicinal Crop Science. 10:132-138.
9 Chang BY, Kim SB, Lee MK, Park H and Kim SY. (2016). Nonclinical safety assessment of Morus alba L. fruits: Study of 90-D toxicity in Sprague Dawley rats and genotoxicity in Salmonella. Journal of Food Science. 81:1328-1335.   DOI
10 Brusick DJ. (2008). A critical review of the genetic toxicity of steviol and steviol glycosides. Food and Chemical Toxicology. 46:S83-S91.
11 Chen PN, Chu SC, Chiou HL, Kuo WH, Chiang CL and Hsieh YS. (2006). Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Letters. 235:248-259.   DOI
12 Lee JJ, Yang H, Yoo YM, Hong SS, Lee D, Lee HJ, Lee HJ, Myung CS, Choi KC and Jeung EB. (2012). Morusinol extracted from Morus alba inhibits arterial thrombosis and modulates platelet activation for the treatment of cardiovascular disease. Journal of Atherosclerosis and Thrombosis. 19:516-522.   DOI
13 Lee JS, Kim YR, Park JM, Ha SJ, Kim YE, Baek NI and Hong EK. (2014). Mulberry fruit extract protects pancreatic ${\beta}$-cells against hydrogen peroxide-induced apoptosis via antioxidative activity. Molecules. 19:8904-8915.   DOI
14 Nam S, Jang HW and Shibamoto T. (2012). Antioxidant activities of extracts from teas prepared from medicinal plants, Morus alba L., Camellia sinensis L., and Cudrania tricuspidata, and their volatile components. Journal of Agricultural Food Chemistry. 60:9097-9105.   DOI
15 Lee YJ, Choi DH, Kim EJ, Kim HY, Kwon TO, Kang DG and Lee HS. (2011). Hypotensive, hypolipidemic, and vascular protective effects of Morus alba L. in rats fed an athergenic diet. The American Journal of Chinese Medicine. 39:39-52.   DOI
16 Lim HJ, Jin HG, Woo ER, Lee SK and Kim HP. (2013). The root barks of Morus alba and the flavonoid constituents inhibit airway inflammation. Journal of Ethnopharmacology. 149:169-175.   DOI
17 Maron DM and Ames BN. (1983). Revised methods for the Salmonella mutagenicity test. Mutation Research / Environment Mutagenesis and Related Subjects. 113:173-215.   DOI
18 Naowaratwattana W, De-Eknamkul W and De Mejia FG. (2010). Phenolic-containing organic extracts of mulberry(Morus alba L.) leaves inhibit HepG2 hepatoma cells through G2/M phase arrest, induction of apoptosis, and inhibition of topoisomerase $II{\alpha}$ activity. Journal of Medicinal Food. 13:1045-1056.   DOI
19 Nattapong S and Omboon L. (2008). A new source of whitening agent from a Thai mulberry plant and its betulinic acid quantitation. Natural Product Research. 22:727-734.   DOI
20 Choi KH, Lee HA, Park MH and Han JS. (2016). Mulberry(Morus alba L.) fruit extract containing anthocyanins improves glycemic control and insulin sensitivity via activation of AMP-activated protein kinase in diabetic C57BL/Ksj-db/db mice. Journal of Medicinal Food. 19:737-745.   DOI
21 Geng CA, Ma YB, Zhang XM, Yao SY, Xue DQ, Zhang RP and Chen JJ. (2012). Mulberrofuran G and isomulberrofuran G from Morus alba L.: Anti-hepatitis B virus activity and mass spectrometric fragmentation. Journal of Agricultural and Food Chemistry. 60:8197-8202.   DOI
22 Greenwell M and Rahman PKSM. (2015). Medicinal plants: Their use in anticancer treatment. International Journal of Pharmaceutical Sciences and Research. 6:4103-4112.
23 Ham SS, Park GG, Park YH and Park WB. (1992). Antimutagenic effect of the extracts of comfrey. Journal of Korean Society of Food Science and Nutrition. 21:539-543.
24 Han HM, Kwon YS and Kim MJ. (2016). Antioxidant and antiproliferative activity of extracts from water chestnut(Trapa Japonica Flerow). Korean Journal of Medicinal Crop Science. 24:14-20.   DOI
25 Harauma A, Murayama T, Ikeyama K, Sano H, Arai H, Takano R, Kita T, Hara S, Kamei K and Yokode M. (2007). Mulberry leaf powder prevents arthertosclerosis in apolipoprotein E-deficient mice. Biochemical and Biophysical Research Communications. 358:751-756.   DOI
26 Hong CE and Lyu SY. (2012). The antimutagenic effect of mistletoe lectin(Viscum album L. var. coloratum agglutinin). Phytotherapy Research. 26:787-790.   DOI
27 Shin PH, Chan YC, Liao JW, Wang MF and Yen GC. (2010). Antioxidant and cognitive promotion effects of anthocyanin-rich mulberry(Morus atropurpurea L.) on senescence-accelerated mice and prevention of Alzheimer's disease. The Journal of Nutritional Biochemistry. 21:598-605.   DOI
28 Horn RC and Vargas VMF. (2008). Mutagenicity and antimutagenicity of teas used in popular medicine in the Salmonella/microsome assay. Toxicology In Vitro. 22:1043-1049.   DOI
29 Jeong ET, Park MY, Lee JG and Chang DS. (1998). Antimicrobial activity and antimutagenesis of cinnamon (Cinnamomum cassia Blume) bark extract. Journal of Food Hygiene and Safety. 13:337-343.
30 Park JH, Jang TW and Lee SH. (2016). Antioxidative activities and inhibition effects on oxidative DNA damage of Valeriana fauriei. Korean Journal of Medicinal Crop Science. 24:464-470.   DOI
31 Wongwattanasathien O, Kangsadalampai K and Tongyonk L. (2010). Antimutagenicity of some flowers grown in Thailand. Food and Chemical Toxicology. 48:1045-1051.   DOI
32 Yang ZG, Matsuzaki K, Takamatsu S and Kitanaka S. (2011). Inhibitory effects of constituents from Morus alba var. multicaulis on differentiation of 3T3-L1 cells and nitric oxide production in RAW264.7 cells. Molecules. 16:6010-6022.   DOI