Browse > Article
http://dx.doi.org/10.7783/KJMCS.2016.24.1.1

Effect of Dendrobium speciosum Var. Aqueous Extract on Loperamide-Induced Constipation in Rats  

Jang, Ji Hun (National Development Institute of Korean Medicine)
Jung, Ho Kyung (National Development Institute of Korean Medicine)
Sim, Mi Ok (National Development Institute of Korean Medicine)
An, Byeong Kwan (National Development Institute of Korean Medicine)
Kim, Min Suk (National Development Institute of Korean Medicine)
Kang, Byoung Man (National Development Institute of Korean Medicine)
Cho, Jung Hee (National Development Institute of Korean Medicine)
Bean, Chul Gu (Research Institute, Gyeongsangnam-do Agricultural Research and Extension Services)
Kim, Seong Cheol (Namhae Sub-Station, NIHHS, RDA)
Jung, Won Seok (National Development Institute of Korean Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.24, no.1, 2016 , pp. 1-6 More about this Journal
Abstract
Background : Irregular meals and insufficient exercise are major modern lifestyle-related risk factors for constipation. This study aimed to examine the effects of the aqueous extract of Dendrobium speciosum var. (DM) on the improvement of intestinal function and prevention of constipation in rats. Methods and Results : Constipation in rat was induced by loperamide (4 mg/kg) injection for 5 days and rats were randomly assigned to the following groups: normal control rats (NOR), constipated rats (LOP induced) and constipated rats supplemented with dulcolax-S (POS), DMSG, 100 mg/kg (DMSG-100), 300 mg/kg (DMSG-300) and 500 mg/kg (DMSG-500). The DMSG groups showed increments in the frequency and amount of stools compared to the LOP group. Loperamide treatment markedly reduced the water content of feces, whereas DMSG administration significantly increased fecal water contents. Moreover, DMSG improved intestinal transit speed in constipated-rats. Alcian blue staining revealed increased mucus production by crypt cells and mucus contents in feces and the mucosal surface. Conclusions : In summary, DM extraction significantly improves intestinal function in rats, which indicates the effectiveness of this extract in the prevention and treatment of constipation.
Keywords
Dendrobium speciosum; Alcian Blue Staining; Constipation; Laxative; Loperamide;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Aichbichler BW, Wenzel HH, Santa Ana CA, Porter JL, Schiller LR and Fordtran JS. (1998). A comparison of stool characteristic from normal and constipated people. Digestive Diseases and Sciences. 43:2353-2362.   DOI
2 Bongers MEJ, Benninga MA, Maurice-Stam H and Grootenhuis MA. (2009). Health-related quality of life in young adults with symptoms of constipation continuing from childhood into adulthood. Health and Quality of Life Outcomes. 7:20 paragraphs. http://hqlo.biomedcentral.com/articles/10.1186/1477-7525-7-20 (cited by 2015 Sep 14).   DOI
3 Cepinskas G, Specian RD and Kvietys PR. (1993). Adaptive cytoprotection in the small intestine: Role of mucus. American Journal of Physiology. 264:G921-G927.
4 Choi JS, Kim JW, Cho HR, Kim KY, Lee JK, Sohn JH and Ku SK. (2014). Laxative effects of fermented rice extract in rats with loperamide-induced constipation. Experimental and Therapeutic Medicine. 8:1847-1854.   DOI
5 Holzer P. (2009). Opioid receptors in the gastrointestinal tract. Regulatory Peptides. 155:11-17.   DOI
6 Hughes S, Higgs NB and Turnberg LA. (1984). Loperamide has antisecretory activity in the human jejunum in vivo. Gut. 25:931-935.   DOI
7 Ito M, Matsuzaki K, Wang J, Daikonya A, Wang NL, Yao XS and Kitanaka S. (2010). New phenanthrenes and stilbenes from Dendrobium loddigesii. Chemical and Pharmaceutical Bulletin. 58:628-633.   DOI
8 Jun DW, Park HY, Lee OY, Lee HL, Yoon BC, Choi HS, Hahm JS, Lee MH, Lee DH and Kee CS. (2006). A population-based study on bowel habits in a Korean community: Prevalence of functional constipation and self-reported constipation. Digestive Diseases and Sciences. 51:1471-1477.   DOI
9 Jung HK, Jang JH, Ko JH, Kang BM, Yeo JH, Cho JH, Cho HW, Bean CG, Kim SC and Jung WS. (2014). Whitening and anti-wrinkle effects of Korean native Dendrobium moniliforme methanol extract. Korean Journal of Medicinal Crop Science. 22:331-338.   DOI
10 Kakino M, Tazawa S, Maruyama H, Tsuruma K, Araki Y, Shimazawa M and Hara H. (2010). Laxative effects of agarwood on low-fiber diet-induced constipation in rats. BMC Complementary and Alternative Medicine. 10:68. http://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-10-68(cited by 2015 Sep 14).   DOI
11 Kim DG, Jin YG, Jin JY, Kim SC, Kim SC, Han CH and Lee YJ. (2011). Effects of the Actindia chinensis on loperamide-induced constipation in rat. Korean Journal of Plant Resources. 24:61-68.   DOI
12 Kim YG, Yang GH and Cho SI. (2005). Anti-oxidative effects of Dendrobii herba on toxic agent induced kidney cell injury. Korea Journal of Herbology. 20:53-60.
13 Lembo A and Camilleri M. (2003). Chronic constipation. New England Journal of Medicine. 349:1360-1368.   DOI
14 Li Y, Wang CL, Wang YJ, Wang FF, Guo SX, Yang JS and Xiao PG. (2009). Four new bibenzyl derivatives from Dendrobium candidum. Chemical and Pharmaceutical Bulletin(Tokyo). 57:997-999.   DOI
15 Mertz H, Naliboff B and Mayer E. (1999). Physiology of refractory chronic constipation. The American Journal of Gastroenterology. 94:609-615.   DOI
16 Miyazawa M, Shimamura H, Nakamura S and Kameoka H. (1997). Antimutagenic activity of gigantol from Dendrobium nobile. Journal of Agricultural and Food Chemistry. 45:2849-2853.   DOI
17 Moretti M, Cossignani L, Messina F, Dominici L, Villarini M, Curini M and Marcotullio MC. (2013). Antigenotoxic effect, composition and antioxidant activity of Dendrobium speciosum. Food Chemistry. 140:660-665.   DOI
18 Park MH, Kwon CJ, Lim SH, Kim KH, Heo NK, Jang HK, Park IJ and Lee KJ. (2011). Effects of dietary fiber isolated from Synurus deltoides on constipation in loperamide-induced rats. Journal of the Korean Society of Food Science and Nutrition. 40:1715-1719.   DOI
19 Shimotoyodome A, Meguro S, Hase T, Tokimitsu I and Sakata T. (2000). Decreased colonic mucus in rats with loperamide-induced constipation. Comparative Biochemistry and Physiology. 126:203-211.   DOI
20 Rao SSC. (2001). Dyssynergic defecation. Gastroenterology Clinics of North America. 30:97-114.   DOI
21 Sohji Y, Kawashima K and Shimizu M. (1978). Pharmacological studies of loperamide, an anti-diarrheal agent. II. Effects on peristalsis of the small intestine and colon in guinea pigs. Folia Pharmacologica Japonica. 74:155-163.   DOI
22 Suzuki M, Hayakawa Y, Aoki K, Nagase H, Nakamura H, Yamada K and Hirata Y. (1973). Stereochemistry of intermediates in the syntheses of Dendrobium alkaloids. Tetrahedron Letters. 14:331-334.   DOI
23 Watanabe T, Nakaya N, Kurashima K, Kuriyama S, Tsubono Y and Tsuji I. (2004). Constipation, laxative use and risk of colorectal cancer: The Miyagi cohort study. European Jouranl of Cancer. 40:2109-2115.   DOI
24 Yang H, Chou GX, Wang ZT, Guo YW, Hu ZB and Xu LS. (2004). Two new compounds from Dendrobium chrysotoxum. Helvetica Chimica Acta. 87:394-399.   DOI
25 Zhang H, Liao W, Chao W, Chen Q, Zeng H, Wu C, Wu S and Ho HI. (2008). Risk factors for sebaceous gland diseases and their relationship to gastrointestinal dysfunction in Han adolescents. The Journal of Dermatology. 35:555-561.   DOI
26 Zhao C, Liu Q, Halaweish F, Shao B, Ye Y and Zhao W. (2003). Copacamphane, picrotoxane and alloaromadendrane sesquiterpene glycosides and phenolic glycosides from Dendrobium moniliforme. Journal of Natural Products. 66:1140-1143.   DOI