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http://dx.doi.org/10.7783/KJMCS.2015.23.4.298

Effect of Dendrobium loddigesii Rolfe Methanol Extract on Melanogenesis in α-MSH Stimulated B16F10 Cells  

Jung, Ho Kyung (Jeollanamdo Development Institute of Traditional Korean Medicine)
Jang, Ji Hun (Jeollanamdo Development Institute of Traditional Korean Medicine)
Sim, Mi Ok (Jeollanamdo Development Institute of Traditional Korean Medicine)
Lee, Ki Ho (Jeollanamdo Development Institute of Traditional Korean Medicine)
Yeo, Jun Hwan (Jeollanamdo Development Institute of Traditional Korean Medicine)
Kang, Byoung Man (Jeollanamdo Development Institute of Traditional Korean Medicine)
Cho, Jung Hee (Jeollanamdo Development Institute of Traditional Korean Medicine)
Bean, Chul Gu (Research Institute, Gyeongsangnamdo Agricultural Research and Extension Services)
Kim, Seong Cheol (Namhae Sub-Station, National Institute of Horticultural and Herbal Science, RDA)
Jung, Won Seok (Jeollanamdo Development Institute of Traditional Korean Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.23, no.4, 2015 , pp. 298-304 More about this Journal
Abstract
Dendrobium loddigesii (DL) is a valuable and versatile herbal medicine with the anecdotal claims of anti-oxidant and anti-inflammation. In the present study, we investigated the whitening effects of DL under various conditions with B16F10 melanoma cells. The DL extract inhibited melanin contents and tyrosinase activity in a dose-dependent manner, compared with untreated group. Treatment of the DL extract effectively suppressed the ${\alpha}$-MSH-stimulated melanin formation, tyrosinase activity and dendrite outgrowth. Moreover, the ${\alpha}$-MSH-induced mRNA expressions of tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2), microphthalmia-associated transcription factor (MITF) and protein expression of tyrosinase were significantly attenuated by DL treatment. These results indicate that DL may be a great cosmeceutical ingredient for its whitening effects.
Keywords
Dendrobium loddigesii; Whitening; Melanin; Tyrosinase;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 Kim TH, Kim JM, Baek JM, Kim TW, Kim DJ, Park JH and Choe M. (2011). Antioxidant and whitening effects of Agrimonia pilosa Ledeb water extract. Korean Journal of Medicinal Crop Science. 19:177-184.   DOI
2 Kim YG, Yang GH and Cho SI. (2005). Anti-oxidant effects of Dendrobii herba on toxic agent induced kidney cell injury. Korean Journal of Herbology. 20:53-60.
3 Li MF, Hirata Y, Xu GJ, Niwa M and Wu HM. (1991). Studies on the chemical constituents of Dendrobium loddigesii Rolfe. Acta Pharmaceutica Sinica. 26:307-310.
4 Lu Y, Kuang M, Hu GP, Wu RB, Wang J, Liu L and Lin YC. (2014). Loddigesiinols G-J: ${\alpha}$-glucosidase inhibitors from Dendrobium loddigesii. Molecules. 19:8544-8555.   DOI
5 Ng TB, Liu J, Wong JH, Ye X, Wing-Sze SC, Tong Y and Zhang KY. (2012). Review of research on Dendrobium, a prized folk medicine. Applied Microbiology and Biotechnology. 93:1795-1803.   DOI
6 Olivares C and Solano F. (2009). New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins. Pigment Cell and Melanoma Research. 22:750-760.   DOI
7 Park EY, Lee SH and Kim YC. (2013a). Whitening efficacy of water-soluble extracts from Prunophora salicina's(Daeseokjosaeng, Purplekin, Formosa) peel. Journal of Investigative Cosmetology. 9:27-32.   DOI
8 Park SA, Park J, Park CI, Jie YJ, Hwang YC, Kim YH, Jeon SH, Lee HM, Ha JH, Kim KJ and Park SN. (2013b). Cellular antioxidant activity and whitening effects of Dendropanax morbifera leaf extracts. Korean Journal of Microbiology and Biotechnology. 41:407-415.   DOI
9 Seiberg M. (2001). Keratinocyte-melanocyte interactions during melanosome transfer. Pigment Cell and Melanoma Research. 14:236-242.   DOI
10 Seo SY. (2001). Screening of tyrosinase inhibitors from oriental herbs. Korean Journal of Plant Resources. 14:32-37.
11 Seo SY, Sharma VK and Sharma N. (2003). Mushroom tyrosinase: Recent prospects. Journal of Agricultural and Food Chemistry. 51:2837-2853.   DOI
12 Tsai AC, Pan SL, Liao CH, Guh JH, Wang SW, Sun HL, Liu YN, Chen CC, Shen CC, Chang YL and Teng CM. (2010). Moscatilin, a bibenzyl derivative from the India orchid Dendrobrium loddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo. Cancer Letters. 292:163-170.   DOI
13 Wu M, Hemesath TJ, Takemoto CM, Horstmann MA, Wells AG, Price ER, Fisher DZ and Fisher DE. (2000). C-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi. Genes and Development. 14:301-312.
14 Yang SY and Boo HO. (2013). Phenolic compounds, antimicrobial effects and tyrosinase inhibition activities of cucumber grown greenhouse according to cultivars and growth stages. Korean Journal of Plant Resources. 26:645-651.   DOI
15 Ye QH, Qin GW and Zhao WM (2002). Immunomodulatory sesquiterpene glycosides from Dendrobium nobile. Phytochemistry. 61:885-890.   DOI
16 Chun HJ, Choi WH, Baek SH and Woo WH. (2002). Effect of quercetin on melanogenesis in melan-a melanocyte cells. Korean Journal of Pharmacognosy. 33:245-251.
17 Ando H, Niki Y, Ito M, Akiyama K, Matsui MS, Yarosh DB and Ichihashi M. (2012). Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake, and dispersion. Journal of Investigative Dermatology. 132:1222-1229.   DOI
18 Bentley NJ, Eisen T and Goding CR. (1994). Melanocytespecific expression of the human tyrosinase promoter: Activation by the microphthalmia gene product and role of the initiator. Molecular and Cellular Biology. 14:7996-8006.   DOI
19 Bradford MM. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72:248-254.   DOI
20 Choi GP, Chung BH, Lee DI, Lee HY, Lee JH and Kim JD. (2002). Screening of inhibitory activities on angiotensin converting enzyme from medicinal plants. Korean Journal of Medicinal Crop Science. 10:399-402.
21 Hemesath TJ, Price ER, Takemoto C, Badalian T and Fisher DE. (1998). MAP kinase links the transcription factor microphthalmia to c-Kit signalling in melanocytes. Nature. 391:298-301.   DOI
22 Ito M, Matsuzaki K, Wang J, Daikonya A, Wang NL, Yao XS and Kitanaka S. (2010). New phenanthrenes and stilbenes from Dendrobium loddigesii. Chemical and Pharmaceutical Bulletin. 58:628-633.   DOI
23 Jeon YS, Jung YJ, Youm JK, Kim YK and Kim SN. (2013). Inhibitory effect of Endarachne binghamiae extract on melanin synthesis. Korean Journal of Plant Resources. 26:526-532.   DOI
24 Kim NY and Lee HY. (2015). Effect of antioxidant and skin whitening of ethanol extracts from ultrasonic pretreated Abeliophyllum distichum Nakai. Korean Journal of Medicinal Crop Science. 23:155-160.   DOI