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http://dx.doi.org/10.7783/KJMCS.2014.22.4.276

Isolation of Polysaccharides Modulating Intestinal Immune System and Single Oral Dose Toxicity Test in Astragalus membranaceus Abovegroud Parts  

Choi, Ri Na (Department of Herbal Medicine Resource, Kangwon National University)
Park, Yeong Chul (GLP Center, Catholic University of Daegu)
Lee, Ji Sun (GLP Center, Catholic University of Daegu)
Kim, Jung Woo (GLP Center, Catholic University of Daegu)
Kim, Jong Bong (GLP Center, Catholic University of Daegu)
Cheoi, Yu Soon (Kangwon Jeongseon Agricultural Extension Center)
Kim, Kwang Ki (Kangwon Jeongseon Agricultural Extension Center)
Lee, Jae Geun (Department of Applied Plant Sciences, Kangwon National University)
Yu, Chang Yeon (Department of Applied Plant Sciences, Kangwon National University)
Kim, Seung Hyn (Department of Applied Bioscience, Konkuk University)
Chung, Ill Min (Department of Applied Bioscience, Konkuk University)
Kim, Jae Kwang (Division of Life Sciences, Incheon National University)
Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.22, no.4, 2014 , pp. 276-288 More about this Journal
Abstract
The six polysaccharide fractions were prepared by chromatographic procedure from the hot water extracts of the aboveground parts of Astragalus membranaceus. These six polysaccharides from aboveground parts of Astragalus membranaceus Bunge were tested for gut-mucosal immune activity and acute toxicity. In a view of molecular weight, the six fractions were estimated to be 75000, 88000, 129000 and 345000 Da, respectively. Component sugar analysis indicated that these fractions were mainly consisted of galactose (46.3 ~ 11.8%) and arabinose (35.4 ~ 9.9%) in addition to glucose, rhamnose, fucose, arabinose, xylose, mannose, glucuronic acid and galacturonic acid. Among the six major purified polysaccharides, AMA-1-b-PS2 showed highest bone merrow cell proliferation and lymphocyte of Peyer's patch stimulating activity. It may be concluded that intestinal immune system modulating activity of aboveground parts from Astragalus membranaceus Bunge is caused by polysaccharides having a polygalacturonan moiety with neutral sugars such as arabinose and galactose. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs. The results indicated that lethal dose 50 ($LD_{50}$) of water extracts from Astragalus membranaceus-aboveground parts was found to be higher than 5000 mg/kg/day in this experiment. From the above results, we may suggest that Astragalus membranaceus-aboveground parts might have useful as a safe material for functional food and pharmaceutics.
Keywords
Astragalus membranaceus; Aboveground Parts; Polysaccharide; Intestinal Immune System Modulating Activity; Single Dose Oral Toxicity;
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Times Cited By KSCI : 7  (Citation Analysis)
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