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http://dx.doi.org/10.7783/KJMCS.2014.22.2.98

Inhibitory Effect of Alopecurus aequalis Sobol Ethanol Extracts on LPS-induced Inflammatory Response in RAW 264.7 Cells  

Jung, Ho Kyung (Jeollanamdo Development Institute of Traditional Korean Medicine)
Kang, Byoung Man (Jeollanamdo Development Institute of Traditional Korean Medicine)
Jang, Ji Hun (Jeollanamdo Development Institute of Traditional Korean Medicine)
Ahn, Byung Kwan (Jeollanamdo Development Institute of Traditional Korean Medicine)
Yeo, Jun Hwan (Jeollanamdo Development Institute of Traditional Korean Medicine)
Jung, Won Seok (Jeollanamdo Development Institute of Traditional Korean Medicine)
Cho, Jung Hee (Jeollanamdo Development Institute of Traditional Korean Medicine)
Kuk, Yong In (Department of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University)
Hyun, Kyu Hwan (Department of Development in Resource Plants, College of Life Science and Natural Resources, Sunchon National University)
Cho, Hyun Woo (Jeollanamdo Development Institute of Traditional Korean Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.22, no.2, 2014 , pp. 98-104 More about this Journal
Abstract
In the present study, we investigated the anti-inflammatory effects by Alopecurus aequalis Sobol on the lipopolysaccharide (LPS)-induced nitric oxide (NO) production by RAW 264.7 cell line. Consistent with these observations, DS reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein levels in a concentration-dependent manner. In addition, the release of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) were also reduced by DS. Moreover, LPS increased expression phosphorylation of $I{\kappa}B{\alpha}$, but DS showed inhibitory effect by reducing LPS-inducible p-$I{\kappa}B{\alpha}$ expression level. These results suggest that the down regulation of iNOS, COX-2, TNF-${\alpha}$, and IL-6 expression by DS are achieved by the downregulation of NF-${\kappa}B$ activity, a transcription factor necessary for pro-inflammatory mediators, and that is also responsible for its anti-inflammatory effects.
Keywords
Alopecurus aequalis Sobol; Inflammation; RAW 264.7 Cell Line; Lipopolysaccharide;
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Times Cited By KSCI : 5  (Citation Analysis)
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