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http://dx.doi.org/10.7783/KJMCS.2013.21.6.444

Comparative Genetic Characteristics of Korean Ginseng using DNA Markers  

Shin, Mi Ran (Department of Herbal Crop Research, NIHHS, RDA)
Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA)
Chung, Jong Wook (National Agrobiodiversity Center, NAAS, RDA)
Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Seung Ho (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA)
Hyun, Dong Yun (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Kee Hong (Department of Herbal Crop Research, NIHHS, RDA)
Moon, Ji Young (Experiment Research Institute of National Agricultural Products Quality Management Service, MIFAFF)
Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University)
Kang, Sung Taek (Department of Crop Science and Biotechnology, Dankook University)
Lee, Dong Jin (Department of Crop Science and Biotechnology, Dankook University)
Bang, Kyong Hwan (Department of Herbal Crop Research, NIHHS, RDA)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.6, 2013 , pp. 444-454 More about this Journal
Abstract
The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study, 18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11 Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of the resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei's genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.
Keywords
Korean Ginseng; Cultivar; Breeding Line; Genetic Characteristics; DNA Marker; RAPD; EST-SSR;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
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1 Bai D, Brandle J and Reeleder R. (1997). Genetic diversity in north America ginseng(Panax quinquefolius L.) grown in Ontario detected by RAPD analysis. Genome. 40:111-115.   DOI   ScienceOn
2 Bang KH, Sung JS, Park CH, Jin DC, Park CG, Yu HS, Park HW and Seong NS. (2004). Discrimination of Atractylodes rhizome white using anatomical characteristics and SCAR markers. Korean Journal of Medicinal Crop Science. 12:53-59.   과학기술학회마을
3 Bang KH, Lee JW, Kim YC, Kim DH, Lee EH and Jeung JU. (2010). Construction of genomic DNA library of Korean ginseng(Panax ginseng C. A. Meyer) and development of sequence-tagged sites. Biological and Pharmaceutical Bulletin. 33:1579-1588.   DOI   ScienceOn
4 Bang KH, Chung JW, Kim YC, Lee JW, Jo IH, Seo AY, Kim OT, Hyun DY, Kim DH and Cha SW. (2011). Development of SSR markers for identification of Korean ginseng(Panax ginseng C. A. Mey.) cultivars. Korean Journal of Medicinal Crop Science. 19:185-190.   과학기술학회마을   DOI   ScienceOn
5 Bang KH, Seo AY, Chung JW, Kim YC, Jo IH, Kim JU, Kim DH, Cha SW, Cho YG and Kim HS. (2012). Analysis of genetic polymorphism and relationship of Korean ginseng cultivars and breeding lines using EST-SSR marker. Korean Journal of Medicinal Crop Science. 20:277-285.   과학기술학회마을   DOI   ScienceOn
6 Bernet GP, Bramardi S, Calvache D, Carbonell EA and Asins MJ. (2003). Applicability of molecular markers in the context of protection of new varieties of cucumber. Plant Breeding. 122:146-152.   DOI   ScienceOn
7 Boehm CL, Harrison HC, Jung G and Nienhuis J. (1999). Organization of American and Asian ginseng germplasm using randomly amplified polymorphic DNA(RAPD) marker. Journal of the American Society for Horticultural Science. 124:252-256.
8 Cui XM, Lo CK, Yip KL, Dong TTX and Tsim KWW. (2003). Authentication of Panax notoginseng by 5S-rRNA spacer domain and random amplified polymorphic DNA(RAPD) analysis. Planta Medica. 69:584-586.   DOI   ScienceOn
9 Devos KM and Gale MD. (1992). The use of random amplified polymorphic DNA markers in wheat. Theoretical and Applied Genetics. 84:567-572.
10 Hamada SR, Petrino MG and Kakunaga T. (1982). A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proceedings of National Academy of Science of the United States of America. 79:6465-6469.   DOI   ScienceOn
11 Kang HW, Park DS, Go SJ and Eun MY. (2002). Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice. Molecules and Cells. 13:281-287.
12 Kang HW and Kang KK. (2008). Genomic characterization of Oryza species-specific CACTA-like transposon element and its application for genomic fingerprinting of rice varieties. Molecular Breeding. 21:283-292.   DOI
13 Kim NH, Ahn IO, Choi HI and Yang TJ. (2012). EST-SSR marker sets for practical authentication of all nine registered ginseng cultivars in Korea. Journal of Ginseng Research. 36:298-307.   과학기술학회마을   DOI   ScienceOn
14 Kwon WS, Lee JH, Park CS and Yang DC. (2003). Breeding process and characteristics of Gopoong, a new variety of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 27:86-91.   과학기술학회마을   DOI   ScienceOn
15 Kwon WS, Moon CH, Kim YT, Lee MG and Choi KT. (1991). Comparisons of growth, crude saponin, ginsenosides, and anthocyanins in superior lines of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 23:219-228.
16 Kwon WS, Lee MG and Choi KT. (2000). Breeding process and characteristics of Yunpoong, a new variety of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 24:1-7.   과학기술학회마을
17 Kwon WS, Lee MG and Lee JH. (2001). Characteristics of flowering and fruiting in new varieties and lines of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 25:41-44.   과학기술학회마을
18 Lee JW, Kim YC, Jo IH, Seo AY, Lee JH, Kim OT, Hyun DY, Cha SW, Bang KH and Cho JH. (2011). Development of an ISSR-derived SCAR marker in Korean ginseng cultivars(Panax ginseng C. A. Meyer). Journal of Ginseng Research. 35:52-59.   과학기술학회마을   DOI   ScienceOn
19 Lewontin RC. (1972). The apportionment of human diversity. Evolutionary Biology. 6:381-398.
20 Lim W, Mudge KW and Weston LA. (2007). Utilization of RAPD markers to assess genetic diversity of wild populations of north American ginseng(Panax quinquefolium). Planta Medica. 73:71-76.   DOI   ScienceOn
21 Ministry of Food and Drug Safety(MFDS). (2013). State of health functional foods in 2012. Ministry of Food and Drug Safety. Osong, Korea.
22 Seo SD, Yuk JA, Cha SK, Kim HH, Seong BJ, Kim SI and Choi JE. (2003). Analysis of diversity of Panax ginseng collected in Korea by RAPD technique. Korean Journal of Medicinal Crop Science. 11:377-384.   과학기술학회마을
23 Mullis KB, Faloona FA, Scharf SJ, Saiki RK, Horn GT and Erlich H. (1986). Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harbor Symposia on Quantitative Biology. 51:263-273.   DOI   ScienceOn
24 Rhim SY, Sohn JK, Ryu TS, Kwon TR, Choi JK and Choi HJ. (2010). Analysis for the major traits and genetic similarity of native ginseng(Panax ginseng C. A. Meyer) collections in Korea. Korean Journal of Breeding. 42:488-494.
25 Rural Development Administration(RDA). (2009). Standard cultivation guidebook for good agricultural practice. Rural Development Administration. Suwon, Korea. p.47-117.
26 Shim YH, Choi JH, Park CD, Lim CJ, Cho JH and Kim HJ. (2003). Molecular differentiation of Panax species by RAPD analysis. Archives of Pharmacal Research. 26:601-605.   과학기술학회마을   DOI   ScienceOn
27 Tautz D and Renz M. (1984). Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Research. 12:4127-4138.   DOI   ScienceOn
28 Williams JGK, Kubelik AR, Livak KJ, Raafalski JA and Tingey SV. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research. 18:6531-6535.   DOI   ScienceOn
29 Xu YH, Jin H, Kim YC, Bang KH, Cha SW and Zhang LX. (2010). Genetic diversity and genetic structures in ginseng landraces(cultivars) by SRAP analysis. Korean Journal of Medicinal Crop Science. 18:180-185.   과학기술학회마을
30 Yang CJ, Wang J, Mu LQ, Li SC, Liu GJ and Hu CQ. (2008). Development of an EST-SSR marker in Panax ginseng. Chinese Journal of Agricultural Biotechnology. 5:175-181.
31 Yang DC and Kim MS. (2003). DNA analysis of ginseng using PCR-aided RFLP technology. Journal of Ginseng Research. 27:146-150.   과학기술학회마을   DOI   ScienceOn
32 Yeh FC, Yang RC, Boyle TBJ, Ye ZH and Mao JX. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta. Canada.