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http://dx.doi.org/10.7783/KJMCS.2013.21.5.361

Antimelanogenic Effect and Whitening of Anthocyanin Rich Fraction from Seeds of Liriope platyphylla  

Choung, Myoung Gun (Department of Herbal Medicine Resource, Kangwon National University)
Hwang, Young Sun (Department of Herbal Medicine Resource, Kangwon National University)
Kim, Gi Ppeum (Department of Herbal Medicine Resource, Kangwon National University)
Ahn, Kyung Geun (Department of Herbal Medicine Resource, Kangwon National University)
Shim, Hoon Seob (Department of Herbal Medicine Resource, Kangwon National University)
Hong, Seung Beom (Department of Herbal Medicine Resource, Kangwon National University)
Choi, Jae Hoo (Department of Applied Plant Sciences, Kangwon National University)
Yu, Chang Yeon (Department of Applied Plant Sciences, Kangwon National University)
Chung, Ill Min (Department of Applied Bioscience, Konkuk University)
Kim, Seung Hyun (Department of Applied Bioscience, Konkuk University)
Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.5, 2013 , pp. 361-371 More about this Journal
Abstract
This study was performed to determine the antimelanogenic effect and tyrosinase inhibitory activities of anthocyanin rich fraction (AN-SLP) from Liriope platyphylla Wang et Tang seeds. Anthocyanins isolated from L. platyphylla seeds revealed the presence of four major anthocyanin components, which were tentatively identified as delphinidin-3-Oglucoside, delphinidin-3-O-rutinoside, petunidin-3-O-rutinoside, and malvidin-3-O-rutinoside using semipreparative HPLC, $^1H$-NMR, $^{13}C$ NMR, FAB-MS and LC/ES-MS. The inhibitory effect of AN-SLP on tyrosinase activity was studied using in vitro (against mushroom tyrosinase) and ex vivo (against B16 melanoma cell tyrosinase) models. Cellular tyrosinase activity was decreased by AN-SLP treatment in B 16 melanoma cells through dose dependent manner, but AN-SLP did not inhibit mushroom tyrosinase and L-DOPA oxidation directly. AN-SLP showed melanin inhibition by 53.2% at 50 ${\mu}g/m{\ell}$ which was 0.7 times more efficient than the antimelanogenic effect of commercial arbutin and kojic acid (36.5%) also did not show cell toxicity. Additionally, AN-SLP inhibited the activity of ${\alpha}$-glucosidase and the glycosylation of tyrosinase in melanoma cell. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. From theses results, we conclude that AN-SLP could be used as anti-melanogenic agent for skin whitening.
Keywords
Anthocyanin Rich Fraction; Antimelanogenic Effect; Seeds of Liriope platyphylla; Tyrosinase Inhibitory Activity;
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Times Cited By KSCI : 6  (Citation Analysis)
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